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You searched for: EV130074 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130074 | 3/3 | Homo sapiens Mus musculus |
NAY |
(d)(U)C Filtration |
Crescitelli R | 2013 | 29% | |
Study summaryFull title
All authors
Crescitelli R, Lässer C, Szabó TG, Kittel A, Eldh M, Dianzani I, Buzás EI, Lötvall J
Journal
J Extracell Vesicles
Abstract
INTRODUCTION: In recent years, there has been an exponential increase in the number of studies aimin (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes / microvesicles / extracellular vesicles / "Apo
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens / Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Filtration steps
0.22µm or 0.2µm
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV130074 | 1/3 | Homo sapiens Mus musculus |
NAY |
(d)(U)C Filtration |
Crescitelli R | 2013 | 14% | |
Study summaryFull title
All authors
Crescitelli R, Lässer C, Szabó TG, Kittel A, Eldh M, Dianzani I, Buzás EI, Lötvall J
Journal
J Extracell Vesicles
Abstract
INTRODUCTION: In recent years, there has been an exponential increase in the number of studies aimin (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes / microvesicles / extracellular vesicles / "Apo
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens / Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: time(min)
20
Filtration steps
> 0.45 µm,
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130074 | 2/3 | Homo sapiens Mus musculus |
NAY |
(d)(U)C Filtration |
Crescitelli R | 2013 | 14% | |
Study summaryFull title
All authors
Crescitelli R, Lässer C, Szabó TG, Kittel A, Eldh M, Dianzani I, Buzás EI, Lötvall J
Journal
J Extracell Vesicles
Abstract
INTRODUCTION: In recent years, there has been an exponential increase in the number of studies aimin (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes / microvesicles / extracellular vesicles / "Apo
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens / Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
40
Filtration steps
> 0.45 µm,
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
1 - 3 of 3 |
EV-TRACK ID | EV130074 | ||
---|---|---|---|
species | Homo sapiens Mus musculus | ||
sample type | Cell culture | ||
cell type | NAY | ||
condition | NAY | ||
separation protocol | (d)(U)C Filtration | (d)(U)C Filtration | (d)(U)C Filtration |
Exp. nr. | 3 | 1 | 2 |
EV-METRIC % | 29 | 14 | 14 |