Search > Results
You searched for: EV130065 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130065 | 2/3 | Mus musculus | BALF |
(d)(U)C DC Filtration IAF |
Kulshreshtha A | 2013 | 43% | |
Study summaryFull title
All authors
Kulshreshtha A, Ahmad T, Agrawal A, Ghosh B
Journal
J Allergy Clin Immunol
Abstract
BACKGROUND: Exosomes are nanovesicles involved in intercellular communication. Their roles in variou (show more...)
EV-METRIC
43% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
BALF
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Filtration IAF Protein markers
EV: CD81/ HSP70/ CD63/ Annexin5/ MHC2
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
BALF
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
Hsp70, CD63
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
MHC2/ Annexin5
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ HSP70/ MHC2/ Annexin5
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV130065 | 3/3 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Kulshreshtha A | 2013 | 33% | |
Study summaryFull title
All authors
Kulshreshtha A, Ahmad T, Agrawal A, Ghosh B
Journal
J Allergy Clin Immunol
Abstract
BACKGROUND: Exosomes are nanovesicles involved in intercellular communication. Their roles in variou (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: CD81/ CD63/ TSG101/ Annexin5/ HSP70
non-EV: Proteomics
no
EV density (g/ml)
1.16-1.21
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Rotor type
SW50.1
Speed (g)
200000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ HSP70/ TSG101/ Annexin5
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Annexin5
Characterization: Particle analysis
None
|
||||||||
EV130065 | 1/3 | Mus musculus | NAY |
(d)(U)C DC Filtration IAF |
Kulshreshtha A | 2013 | 0% | |
Study summaryFull title
All authors
Kulshreshtha A, Ahmad T, Agrawal A, Ghosh B
Journal
J Allergy Clin Immunol
Abstract
BACKGROUND: Exosomes are nanovesicles involved in intercellular communication. Their roles in variou (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Filtration IAF Protein markers
EV: Alix/ TSG101
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
Hsp70, CD63
Characterization: Protein analysis
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Alix/ TSG101
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
Characterization: Particle analysis
None
|
||||||||
1 - 3 of 3 |
EV-TRACK ID | EV130065 | ||
---|---|---|---|
species | Mus musculus | Homo sapiens | Mus musculus |
sample type | BALF | Cell culture | Cell culture |
cell type | NA | NAY | NAY |
medium | EV Depleted | EV Depleted | |
condition | NAY | NAY | NAY |
separation protocol | (d)(U)C DC Filtration IAF | (d)(U)C DG Filtration | (d)(U)C DC Filtration IAF |
Exp. nr. | 2 | 3 | 1 |
EV-METRIC % | 43 | 33 | 0 |