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You searched for: EV130050 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130050 | 1/1 | Shewanella vesiculosa | Bacteria |
(d)(U)C DG Filtration UF |
Pérez-Cruz C | 2013 | 43% | |
Study summaryFull title
All authors
Pérez-Cruz C, Carrión O, Delgado L, Martinez G, López-Iglesias C, Mercade E
Journal
Appl Environ Microbiol
Abstract
Outer membrane vesicles (OMVs) from Gram-negative bacteria are known to be involved in lateral DNA t (show more...)
EV-METRIC
43% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
DG Filtration UF Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Shewanella vesiculosa
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: time(min)
60
Wash: volume per pellet (ml)
25
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
10
Highest density fraction
35
Orientation
Bottom-up
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM/ cryo EM
EM protein
DNA
Image type
Close-up, Wide-field
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EV-TRACK ID | EV130050 |
---|---|
species | Shewanella vesiculosa |
sample type | Bacteria |
condition | NAY |
separation protocol | (d)(U)C DG Filtration UF |
Exp. nr. | 1 |
EV-METRIC % | 43 |