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You searched for: EV130047 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130047 | 1/1 | Mus musculus | NAY |
(d)(U)C DG Filtration |
Näslund TI | 2013 | 57% | |
Study summaryFull title
All authors
Näslund TI, Gehrmann U, Qazi KR, Karlsson MC, Gabrielsson S
Journal
J Immunol
Abstract
Exosomes are secreted membrane nanovesicles of endosomal origin and are considered potential cancer (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: CD81/ MHC2/ CD9
non-EV: CD80/ CD54/ CD86 Proteomics
no
EV density (g/ml)
1.11-1.19
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
130
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Speed (g)
80000
Filtration steps
0.22µm or 0.2µm
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
MHC2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
MHC2
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
Characterization: Particle analysis
DLS
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1 - 1 of 1 |
EV-TRACK ID | EV130047 |
---|---|
species | Mus musculus |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C DG Filtration |
Exp. nr. | 1 |
EV-METRIC % | 57 |