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You searched for: EV130045 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130045 | 1/1 | Homo sapiens | Urine | (d)(U)C | Lv LL | 2013 | 43% | |
Study summaryFull title
All authors
Lv LL, Cao Y, Liu D, Xu M, Liu H, Tang RN, Ma KL, Liu BC
Journal
Int J Biol Sci
Abstract
Recent studies indicate that microRNA (miRNA) is contained within exosome. Here we sought to optimiz (show more...)
EV-METRIC
43% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes / microvesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
TEM measurements
30-120
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
EM protein
AQP2; CD9; Alix
Image type
Close-up, Wide-field
Report size (nm)
30-120
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1 - 1 of 1 |
EV-TRACK ID | EV130045 |
---|---|
species | Homo sapiens |
sample type | Urine |
condition | NAY |
separation protocol | (d)(U)C |
Exp. nr. | 1 |
EV-METRIC % | 43 |