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You searched for: EV120143 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120143 | 1/1 | Klebsiella pneumoniae | Bacteria |
(d)(U)C Filtration UF |
Lee JC | 2012 | 14% | |
Study summaryFull title
All authors
Lee JC, Lee EJ, Lee JH, Jun SH, Choi CW, Kim SI, Kang SS, Hyun S
Journal
FEMS Microbiol Lett
Abstract
Outer membrane vesicles (OMVs) derived from pathogenic Gram-negative bacteria are an important vehic (show more...)
EV-METRIC
14% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Klebsiella pneumoniae
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
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1 - 1 of 1 |
EV-TRACK ID | EV120143 |
---|---|
species | Klebsiella pneumoniae |
sample type | Bacteria |
condition | NAY |
separation protocol | (d)(U)C Filtration UF |
Exp. nr. | 1 |
EV-METRIC % | 14 |