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You searched for: EV120109 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120109 | 2/2 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Yoo CE | 2012 | 14% | |
Study summaryFull title
All authors
Yoo CE, Kim G, Kim M, Park D, Kang HJ, Lee M, Huh N
Journal
Anal Biochem
Abstract
A direct extraction method was developed for exosomal microRNAs. After isolation of exosomes from hu (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Bottom-up
Filtration steps
0.22µm or 0.2µm
|
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EV120109 | 1/2 | Homo sapiens | Serum | IAF | Yoo CE | 2012 | 0% | |
Study summaryFull title
All authors
Yoo CE, Kim G, Kim M, Park D, Kang HJ, Lee M, Huh N
Journal
Anal Biochem
Abstract
A direct extraction method was developed for exosomal microRNAs. After isolation of exosomes from hu (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Immunoaffinity capture
Selected surface protein(s)
EpCAM, CD63
Characterization: Particle analysis
DLS
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1 - 2 of 2 |
EV-TRACK ID | EV120109 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | Serum |
cell type | NAY | NA |
medium | serum free | |
condition | NAY | NAY |
separation protocol | (d)(U)C DG Filtration | IAF |
Exp. nr. | 2 | 1 |
EV-METRIC % | 14 | 0 |