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You searched for: EV110002 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110002 | 1/1 | Homo sapiens Canis familiaris |
NAY |
(d)(U)C DG Filtration |
Higginbotham JN | 2011 | 67% | |
Study summaryFull title
All authors
Higginbotham JN, Demory Beckler M, Gephart JD, Franklin JL, Bogatcheva G, Kremers GJ, Piston DW, Ayers GD, McConnell RE, Tyska MJ, Coffey RJ
Journal
Curr Biol
Abstract
Autocrine, paracrine, and juxtacrine are recognized modes of action for mammalian EGFR ligands inclu (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: TSG101/ Flotilin1/ ADAM10/ ADAM17/ HSP70/ CD9
non-EV: Cell organelle protein Proteomics
no
TEM measurements
51.3(median)
Show all info
Study aim
Function
Sample
Species
Homo sapiens / Canis familiaris
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Density gradient
Only used for validation of main results
Yes
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ Flotilin1/ HSP70/ TSG101/ ADAM10/ ADAM17
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
ADAM10/ ADAM17
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
Report size (nm)
51.3(median)
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EV-TRACK ID | EV110002 |
---|---|
species | Homo sapiens Canis familiaris |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C DG Filtration |
Exp. nr. | 1 |
EV-METRIC % | 67 |