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You searched for: 2017 (Year of publication)
Showing 51 - 100 of 133
Showing 51 - 100 of 133
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV170047 | 5/8 | Homo sapiens | Cell culture supernatant | dUC (Differential) (ultra)centrifugation |
Soekmadji C | 2017 | 44% | |
Study summaryFull title
All authors
Soekmadji C, Riches JD, Russell PJ, Ruelcke JE, McPherson S, Wang C, Hovens CM, Corcoran NM, Hill MM, Nelson CC
Journal
Oncotarget
Abstract
Proliferation and maintenance of both normal and prostate cancer (PCa) cells is highly regulated by (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC + (Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101/ PSA/ CD9
non-EV: GAPDH Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
LNCaP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
120
Pelleting: speed (g)
100000
Wash: time (min)
90
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Concentration
0.005
Western Blot
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD9/ TSG101
Not detected EV-associated proteins
PSA
Not detected contaminants
GAPDH
Characterization: Particle analysis
TRPS
Report type
Size range/distribution,Mode
Reported size (nm)
150
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170026 | 1/1 | Mus musculus | Cell culture supernatant | DG dUC Filtration DC |
Prakash Gangadaran | 2017 | 44% | |
Study summaryFull title
All authors
Gangadaran P, Rajendran RL, Lee HW, Kalimuthu S, Hong CM, Jeong SY, Lee SW, Lee J, Ahn BC
Journal
J Control Release
Abstract
Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) are potential therapies for (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG + dUC + Filtration + DC
Adj. k-factor
253.9 (pelleting) / 253.9 (washing)
Protein markers
EV: Alix/ CD63
non-EV: calnexin/ cytochrome c/ GM130/ cytochromec Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
bone marrow-derived mesenchymal stem cells
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
60
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
253.9
Wash: time (min)
60
Wash: Rotor Type
SW 28
Wash: speed (g)
100000
Wash: adjusted k-factor
253.9
Density cushion
Density medium
Iodixanol
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Alix, CD63
Not detected contaminants
GM130, calnexin, cytochrome c
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
135
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170021 | 1/2 | Homo sapiens | Cell culture supernatant | DG dUC |
Liem, Michael | 2017 | 44% | |
Study summaryFull title
All authors
Liem M, Ang CS, Mathivanan S
Journal
Proteomics
Abstract
Epidemiological studies suggest that diabetes and obesity increases the risk of colorectal cancer (C (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Insulin induced
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG + dUC
Adj. k-factor
253.9 (pelleting) / 89.2 (washing)
Protein markers
EV: TSG101/ TSG101,AKT,pAKT,beta-actin,FAT1,p-cadherin/ AKT/ Alix/ FAT1/ pAKT/ beta-actin/ p-cadherin
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Insulin induced
EV-producing cells
LIM1215
EV-harvesting Medium
Serum free medium
Cell viability
98
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
60
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
253.9
Wash: time (min)
60
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Wash: adjusted k-factor
89.20
Characterization: Protein analysis
Protein Concentration Method
Densitometry (SYPRO Ruby)
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix, TSG101,AKT,pAKT,beta-actin,FAT1,p-cadherin
Proteomics database
Yes
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
50-150
EV concentration
Yes
Particle yield
3.08E+07 particles/million cells
|
||||||||
EV170021 | 2/2 | Homo sapiens | Cell culture supernatant | dUC | Liem, Michael | 2017 | 44% | |
Study summaryFull title
All authors
Liem M, Ang CS, Mathivanan S
Journal
Proteomics
Abstract
Epidemiological studies suggest that diabetes and obesity increases the risk of colorectal cancer (C (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC
Adj. k-factor
253.9 (pelleting) / 89.2 (washing)
Protein markers
EV: TSG101/ AKT/ Alix/ FAT1/ beta-actin/ p-cadherin/ TSG101,AKT,beta-actin,FAT1,p-cadherin
non-EV: None Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
LIM1215
EV-harvesting Medium
Serum free medium
Cell viability
98
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
60
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
253.9
Wash: time (min)
60
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Wash: adjusted k-factor
89.20
Characterization: Protein analysis
Protein Concentration Method
Densitometry (SYPRO Ruby)
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix, TSG101,AKT,beta-actin,FAT1,p-cadherin
Proteomics database
Yes
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
50-150
EV concentration
Yes
Particle yield
1.85E+07 particles/million cells
|
||||||||
EV170003 | 1/1 | Mus musculus | Cell culture supernatant | dUC | Nager AR | 2017 | 44% | |
Study summaryFull title
All authors
Nager AR, Goldstein JS, Herranz-Pérez V, Portran D, Ye F, Garcia-Verdugo JM, Nachury MV
Journal
Cell
Abstract
Signaling receptors dynamically exit cilia upon activation of signaling pathways such as Hedgehog. H (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
GPCR signaling in BBS mutant
Focus vesicles
Ciliary Ectosome
Separation protocol
Separation protocol
dUC
Adj. k-factor
253.9 (pelleting) / 99.86 (washing)
Protein markers
EV: CD81/ Arl13B/ BiotinylatedGPCRs
non-EV: None Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Sample Condition
GPCR signaling in BBS mutant
EV-producing cells
mIMCD3
EV-harvesting Medium
Serum-containing medium
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
90
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
253.9
Wash: time (min)
90
Wash: Rotor Type
TLS-55
Wash: speed (g)
100000
Wash: adjusted k-factor
99.86
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
CD81, Arl13B
Characterization: Particle analysis
EM
EM-type
Transmission-EM/ Immune-EM
Proteïns
Biotinylated GPCRs
Image type
Close-up, Wide-field
Report size (nm)
70-120
|
||||||||
EV170067 | 4/6 | Homo sapiens | Cell culture supernatant | (Differential) (ultra)centrifugation Filtration Density gradient |
Lässer C | 2017 | 43% | |
Study summaryFull title
All authors
Lässer C, Shelke GV, Yeri A, Kim DK, Crescitelli R, Raimondo S, Sjöstrand M, Gho YS, Van Keuren Jensen K, Lötvall J.
Journal
RNA Biol
Abstract
Cells secrete extracellular RNA (exRNA) to their surrounding environment and exRNA has been found in (show more...)
EV-METRIC
43% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Extracellular vesicle-like structures
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation + Filtration + Density gradient
Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.09 - 1.31
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
HMC-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
120000
Density gradient
Density medium
Sucrose
Type
Discontinuous
Number of initial discontinuous layers
16
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
35
Sample volume (mL)
2
Orientation
Top-down
Rotor type
SW 32 Ti
Speed (g)
175000
Duration (min)
960
Fraction volume (mL)
3.9
Fraction processing
Centrifugation
Pelleting: duration (min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
120000
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
Density
Used subtypes
1.24 - 1.31 g/mL
Protein Concentration Method
Not determined
EM
EM-type
Immuno-EM
Proteïns
CD63
Image type
Wide-field
Report size (nm)
51
|
||||||||
EV170067 | 5/6 | Homo sapiens | Cell culture supernatant | (Differential) (ultra)centrifugation Density gradient Filtration |
Lässer C | 2017 | 43% | |
Study summaryFull title
All authors
Lässer C, Shelke GV, Yeri A, Kim DK, Crescitelli R, Raimondo S, Sjöstrand M, Gho YS, Van Keuren Jensen K, Lötvall J.
Journal
RNA Biol
Abstract
Cells secrete extracellular RNA (exRNA) to their surrounding environment and exRNA has been found in (show more...)
EV-METRIC
43% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Extracellular vesicle-like structures
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation + Density gradient + Filtration
Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.09 - 1.31
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
TF-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
120000
Density gradient
Density medium
Sucrose
Type
Discontinuous
Number of initial discontinuous layers
16
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
35
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
175000
Duration (min)
960
Fraction volume (mL)
3.9
Fraction processing
Centrifugation
Pelleting: duration (min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
120000
Filtration steps
0.22µm or 0.2µm
Protein Concentration Method
Not determined
|
||||||||
EV170067 | 6/6 | Homo sapiens | Cell culture supernatant | (Differential) (ultra)centrifugation Density gradient |
Lässer C | 2017 | 43% | |
Study summaryFull title
All authors
Lässer C, Shelke GV, Yeri A, Kim DK, Crescitelli R, Raimondo S, Sjöstrand M, Gho YS, Van Keuren Jensen K, Lötvall J.
Journal
RNA Biol
Abstract
Cells secrete extracellular RNA (exRNA) to their surrounding environment and exRNA has been found in (show more...)
EV-METRIC
43% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Extracellular vesicle-like structures
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation + Density gradient
Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.09 - 1.31
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
TF-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
120000
Density gradient
Density medium
Sucrose
Type
Discontinuous
Number of initial discontinuous layers
16
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
35
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
175000
Duration (min)
960
Fraction volume (mL)
3.9
Fraction processing
Centrifugation
Pelleting: duration (min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
120000
Protein Concentration Method
Not determined
|
||||||||
EV170054 | 1/5 | Homo sapiens | Urine | dUC | Gheinani AH | 2017 | 42% | |
Study summaryFull title
All authors
Gheinani AH, Vögeli M, Baumgartner U, Vassella E, Draeger A, Burkhard FC, Monastyrskaya K
Journal
Sci Rep
Abstract
Circulating miRNAs are detected in extracellular space and body fluids such as urine. Circulating RN (show more...)
EV-METRIC
42% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC
Adj. k-factor
174.8 (pelleting) / 174.8 (washing)
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Sample Condition
Control condition
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
174.8
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
120000
Wash: adjusted k-factor
174.8
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Protein Concentration
15.9
Characterization: Particle analysis
NTA
Report type
Mode;mean;size range/distribution;D10;D50;D90
Reported size (nm)
174
EV concentration
Yes
Particle yield
see Fig1A
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170006 | 1/8 | Homo sapiens | Serum | dUC | Julich-Haertel H | 2017 | 37% | |
Study summaryFull title
All authors
Julich-Haertel H, Urban SK, Krawczyk M, Willms A, Jankowski K, Patkowski W, Kruk B, Krasnodębski M, Ligocka J, Schwab R, Richardsen I, Schaaf S, Klein A, Gehlert S, Sänger H, Casper M, Banales JM, Schuppan D, Milkiewicz P, Lammert F, Krawczyk M, Lukacs-Kornek V, Kornek M
Journal
J Hepatol
Abstract
BACKGROUND AND AIMS:
we previously reported that large extracellular vesicles, specifically AnnexinV (show more...)
EV-METRIC
37% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
microparticle
Separation protocol
Separation protocol
dUC
Adj. k-factor
284.4 (pelleting)
Protein markers
EV: CD147/ EpCAM/ ASGPR1/ ANXA5/ CD133
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Sample Condition
Control condition
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting: time(min)
60
Pelleting: rotor type
FA-45-24-11
Pelleting: speed (g)
20000
Pelleting: adjusted k-factor
284.4
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
MACSQuant® Analyzer 10
Calibration bead size
0.2,0.5
Extra information
We characterised various tumor-associated MPs (taMPs) in serum from various cancer patients aiming for the detection of liver cancer and differentiation from healthy subjects and other non-liver cancer entities. This led to several useful antigen combinations on taMPs that must be present simultaneously on the surface of the same MP in order to be accounted. That means, we reported several MP surface antigen combinations for the detection and differentiation of liver cancer (here: HCC and CCA).
|
||||||||
EV170006 | 2/8 | Homo sapiens | Serum | dUC | Julich-Haertel H | 2017 | 37% | |
Study summaryFull title
All authors
Julich-Haertel H, Urban SK, Krawczyk M, Willms A, Jankowski K, Patkowski W, Kruk B, Krasnodębski M, Ligocka J, Schwab R, Richardsen I, Schaaf S, Klein A, Gehlert S, Sänger H, Casper M, Banales JM, Schuppan D, Milkiewicz P, Lammert F, Krawczyk M, Lukacs-Kornek V, Kornek M
Journal
J Hepatol
Abstract
BACKGROUND AND AIMS:
we previously reported that large extracellular vesicles, specifically AnnexinV (show more...)
EV-METRIC
37% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Inguinal hernia
Focus vesicles
microparticle
Separation protocol
Separation protocol
dUC
Adj. k-factor
284.4 (pelleting)
Protein markers
EV: CD147/ EpCAM/ ASGPR1/ ANXA5/ CD133
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Sample Condition
Inguinal hernia
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting: time(min)
60
Pelleting: rotor type
FA-45-24-11
Pelleting: speed (g)
20000
Pelleting: adjusted k-factor
284.4
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
MACSQuant® Analyzer 10
Calibration bead size
0.2,0.5
Extra information
We characterised various tumor-associated MPs (taMPs) in serum from various cancer patients aiming for the detection of liver cancer and differentiation from healthy subjects and other non-liver cancer entities. This led to several useful antigen combinations on taMPs that must be present simultaneously on the surface of the same MP in order to be accounted. That means, we reported several MP surface antigen combinations for the detection and differentiation of liver cancer (here: HCC and CCA).
|
||||||||
EV170006 | 3/8 | Homo sapiens | Serum | dUC | Julich-Haertel H | 2017 | 37% | |
Study summaryFull title
All authors
Julich-Haertel H, Urban SK, Krawczyk M, Willms A, Jankowski K, Patkowski W, Kruk B, Krasnodębski M, Ligocka J, Schwab R, Richardsen I, Schaaf S, Klein A, Gehlert S, Sänger H, Casper M, Banales JM, Schuppan D, Milkiewicz P, Lammert F, Krawczyk M, Lukacs-Kornek V, Kornek M
Journal
J Hepatol
Abstract
BACKGROUND AND AIMS:
we previously reported that large extracellular vesicles, specifically AnnexinV (show more...)
EV-METRIC
37% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Colon carcinoma
Focus vesicles
microparticle
Separation protocol
Separation protocol
dUC
Adj. k-factor
284.4 (pelleting)
Protein markers
EV: CD147/ EpCAM/ ASGPR1/ ANXA5/ CD133
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Sample Condition
Colon carcinoma
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting: time(min)
60
Pelleting: rotor type
FA-45-24-11
Pelleting: speed (g)
20000
Pelleting: adjusted k-factor
284.4
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
MACSQuant® Analyzer 10
Calibration bead size
0.2,0.5
Extra information
We characterised various tumor-associated MPs (taMPs) in serum from various cancer patients aiming for the detection of liver cancer and differentiation from healthy subjects and other non-liver cancer entities. This led to several useful antigen combinations on taMPs that must be present simultaneously on the surface of the same MP in order to be accounted. That means, we reported several MP surface antigen combinations for the detection and differentiation of liver cancer (here: HCC and CCA).
|
||||||||
EV170006 | 4/8 | Homo sapiens | Serum | dUC | Julich-Haertel H | 2017 | 37% | |
Study summaryFull title
All authors
Julich-Haertel H, Urban SK, Krawczyk M, Willms A, Jankowski K, Patkowski W, Kruk B, Krasnodębski M, Ligocka J, Schwab R, Richardsen I, Schaaf S, Klein A, Gehlert S, Sänger H, Casper M, Banales JM, Schuppan D, Milkiewicz P, Lammert F, Krawczyk M, Lukacs-Kornek V, Kornek M
Journal
J Hepatol
Abstract
BACKGROUND AND AIMS:
we previously reported that large extracellular vesicles, specifically AnnexinV (show more...)
EV-METRIC
37% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Hepatocellular carcinoma
Focus vesicles
microparticle
Separation protocol
Separation protocol
dUC
Adj. k-factor
284.4 (pelleting)
Protein markers
EV: CD147/ EpCAM/ ASGPR1/ ANXA5/ CD133
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Sample Condition
Hepatocellular carcinoma
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting: time(min)
60
Pelleting: rotor type
FA-45-24-11
Pelleting: speed (g)
20000
Pelleting: adjusted k-factor
284.4
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
MACSQuant® Analyzer 10
Calibration bead size
0.2,0.5
Extra information
We characterised various tumor-associated MPs (taMPs) in serum from various cancer patients aiming for the detection of liver cancer and differentiation from healthy subjects and other non-liver cancer entities. This led to several useful antigen combinations on taMPs that must be present simultaneously on the surface of the same MP in order to be accounted. That means, we reported several MP surface antigen combinations for the detection and differentiation of liver cancer (here: HCC and CCA).
|
||||||||
EV170006 | 5/8 | Homo sapiens | Serum | dUC | Julich-Haertel H | 2017 | 37% | |
Study summaryFull title
All authors
Julich-Haertel H, Urban SK, Krawczyk M, Willms A, Jankowski K, Patkowski W, Kruk B, Krasnodębski M, Ligocka J, Schwab R, Richardsen I, Schaaf S, Klein A, Gehlert S, Sänger H, Casper M, Banales JM, Schuppan D, Milkiewicz P, Lammert F, Krawczyk M, Lukacs-Kornek V, Kornek M
Journal
J Hepatol
Abstract
BACKGROUND AND AIMS:
we previously reported that large extracellular vesicles, specifically AnnexinV (show more...)
EV-METRIC
37% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Liver tumour
Focus vesicles
microparticle
Separation protocol
Separation protocol
dUC
Adj. k-factor
284.4 (pelleting)
Protein markers
EV: CD147/ EpCAM/ ASGPR1/ ANXA5/ CD133
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Sample Condition
Liver tumour
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting: time(min)
60
Pelleting: rotor type
FA-45-24-11
Pelleting: speed (g)
20000
Pelleting: adjusted k-factor
284.4
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
MACSQuant® Analyzer 10
Calibration bead size
0.2,0.5
Extra information
We characterised various tumor-associated MPs (taMPs) in serum from various cancer patients aiming for the detection of liver cancer and differentiation from healthy subjects and other non-liver cancer entities. This led to several useful antigen combinations on taMPs that must be present simultaneously on the surface of the same MP in order to be accounted. That means, we reported several MP surface antigen combinations for the detection and differentiation of liver cancer (here: HCC and CCA).
|
||||||||
EV170006 | 6/8 | Homo sapiens | Serum | dUC | Julich-Haertel H | 2017 | 37% | |
Study summaryFull title
All authors
Julich-Haertel H, Urban SK, Krawczyk M, Willms A, Jankowski K, Patkowski W, Kruk B, Krasnodębski M, Ligocka J, Schwab R, Richardsen I, Schaaf S, Klein A, Gehlert S, Sänger H, Casper M, Banales JM, Schuppan D, Milkiewicz P, Lammert F, Krawczyk M, Lukacs-Kornek V, Kornek M
Journal
J Hepatol
Abstract
BACKGROUND AND AIMS:
we previously reported that large extracellular vesicles, specifically AnnexinV (show more...)
EV-METRIC
37% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Cirrhosis
Focus vesicles
microparticle
Separation protocol
Separation protocol
dUC
Adj. k-factor
284.4 (pelleting)
Protein markers
EV: CD147/ EpCAM/ ASGPR1/ ANXA5/ CD133
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Sample Condition
Cirrhosis
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting: time(min)
60
Pelleting: rotor type
FA-45-24-11
Pelleting: speed (g)
20000
Pelleting: adjusted k-factor
284.4
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
MACSQuant® Analyzer 10
Calibration bead size
0.2,0.5
Extra information
We characterised various tumor-associated MPs (taMPs) in serum from various cancer patients aiming for the detection of liver cancer and differentiation from healthy subjects and other non-liver cancer entities. This led to several useful antigen combinations on taMPs that must be present simultaneously on the surface of the same MP in order to be accounted. That means, we reported several MP surface antigen combinations for the detection and differentiation of liver cancer (here: HCC and CCA).
|
||||||||
EV170006 | 7/8 | Homo sapiens | Serum | dUC | Julich-Haertel H | 2017 | 37% | |
Study summaryFull title
All authors
Julich-Haertel H, Urban SK, Krawczyk M, Willms A, Jankowski K, Patkowski W, Kruk B, Krasnodębski M, Ligocka J, Schwab R, Richardsen I, Schaaf S, Klein A, Gehlert S, Sänger H, Casper M, Banales JM, Schuppan D, Milkiewicz P, Lammert F, Krawczyk M, Lukacs-Kornek V, Kornek M
Journal
J Hepatol
Abstract
BACKGROUND AND AIMS:
we previously reported that large extracellular vesicles, specifically AnnexinV (show more...)
EV-METRIC
37% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Cholangiocarcinoma
Focus vesicles
microparticle
Separation protocol
Separation protocol
dUC
Adj. k-factor
284.4 (pelleting)
Protein markers
EV: ASGPR1/ EpCAM/ ANXA5/ CD133
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Sample Condition
Cholangiocarcinoma
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting: time(min)
60
Pelleting: rotor type
FA-45-24-11
Pelleting: speed (g)
20000
Pelleting: adjusted k-factor
284.4
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
MACSQuant® Analyzer 10
Calibration bead size
0.2,0.5
Extra information
We characterised various tumor-associated MPs (taMPs) in serum from various cancer patients aiming for the detection of liver cancer and differentiation from healthy subjects and other non-liver cancer entities. This led to several useful antigen combinations on taMPs that must be present simultaneously on the surface of the same MP in order to be accounted. That means, we reported several MP surface antigen combinations for the detection and differentiation of liver cancer (here: HCC and CCA).
|
||||||||
EV170006 | 8/8 | Homo sapiens | Serum | dUC | Julich-Haertel H | 2017 | 37% | |
Study summaryFull title
All authors
Julich-Haertel H, Urban SK, Krawczyk M, Willms A, Jankowski K, Patkowski W, Kruk B, Krasnodębski M, Ligocka J, Schwab R, Richardsen I, Schaaf S, Klein A, Gehlert S, Sänger H, Casper M, Banales JM, Schuppan D, Milkiewicz P, Lammert F, Krawczyk M, Lukacs-Kornek V, Kornek M
Journal
J Hepatol
Abstract
BACKGROUND AND AIMS:
we previously reported that large extracellular vesicles, specifically AnnexinV (show more...)
EV-METRIC
37% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Non small cell lung carcinoma
Focus vesicles
microparticle
Separation protocol
Separation protocol
dUC
Adj. k-factor
284.4 (pelleting)
Protein markers
EV: ASGPR1/ EpCAM/ ANXA5/ CD133
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Sample Condition
Non small cell lung carcinoma
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting: time(min)
60
Pelleting: rotor type
FA-45-24-11
Pelleting: speed (g)
20000
Pelleting: adjusted k-factor
284.4
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
MACSQuant® Analyzer 10
Calibration bead size
0.2,0.5
Extra information
We characterised various tumor-associated MPs (taMPs) in serum from various cancer patients aiming for the detection of liver cancer and differentiation from healthy subjects and other non-liver cancer entities. This led to several useful antigen combinations on taMPs that must be present simultaneously on the surface of the same MP in order to be accounted. That means, we reported several MP surface antigen combinations for the detection and differentiation of liver cancer (here: HCC and CCA).
|
||||||||
EV170070 | 1/1 | Homo sapiens | Cell culture supernatant | (Differential) (ultra)centrifugation Filtration |
Tsang EK | 2017 | 33% | |
Study summaryFull title
All authors
Tsang EK, Abell NS, Li X, Anaya V, Karczewski KJ, Knowles DA, Sierra RG, Smith KS, Montgomery SB.
Journal
G3 (Bethesda)
Abstract
Exosomes are small extracellular vesicles that carry heterogeneous cargo, including RNA, between cel (show more...)
EV-METRIC
33% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Epstein-Barr virus-transformed
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation + Filtration
Protein markers
EV: HSP70
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Epstein-Barr virus-transformed
EV-producing cells
CEPH/UTAH family PEDIGREE 1463 peripheral blood B lymphocyte
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
120000
Wash: time (min)
70
Wash: Rotor Type
TLA-100.3
Wash: speed (g)
120000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
HSP70
Not detected contaminants
Calnexin
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
107
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
100
|
||||||||
EV170054 | 2/5 | Homo sapiens | Urine | SEC dUC Filtration Ultrafiltration UF |
Gheinani AH | 2017 | 33% | |
Study summaryFull title
All authors
Gheinani AH, Vögeli M, Baumgartner U, Vassella E, Draeger A, Burkhard FC, Monastyrskaya K
Journal
Sci Rep
Abstract
Circulating miRNAs are detected in extracellular space and body fluids such as urine. Circulating RN (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
SEC + dUC + Filtration + Ultrafiltration + UF
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Sample Condition
Control condition
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Polyethersulfone (PES)
Size-exclusion chromatography
Total column volume (mL)
23
Sample volume/column (mL)
0.4
Resin type
Sepharose CL-2B
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Protein Concentration
5.7
Characterization: Particle analysis
NTA
Report type
Mode;mean;size range/distribution;D10;D50;D90
Reported size (nm)
113
EV concentration
Yes
Particle yield
see Fig1A
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170054 | 3/5 | Homo sapiens | Urine | PEG precipitation dUC Other |
Gheinani AH | 2017 | 33% | |
Study summaryFull title
All authors
Gheinani AH, Vögeli M, Baumgartner U, Vassella E, Draeger A, Burkhard FC, Monastyrskaya K
Journal
Sci Rep
Abstract
Circulating miRNAs are detected in extracellular space and body fluids such as urine. Circulating RN (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
PEG precipitation + dUC + Other
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Sample Condition
Control condition
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Other
Name other separation method
PEG precipitation
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Protein Concentration
8.4
Characterization: Particle analysis
NTA
Report type
Mode;mean;size range/distribution;D10;D50;D90
Reported size (nm)
138
EV concentration
Yes
Particle yield
see Fig1A
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170054 | 4/5 | Homo sapiens | Urine | PEG precipitation SEC dUC Other |
Gheinani AH | 2017 | 33% | |
Study summaryFull title
All authors
Gheinani AH, Vögeli M, Baumgartner U, Vassella E, Draeger A, Burkhard FC, Monastyrskaya K
Journal
Sci Rep
Abstract
Circulating miRNAs are detected in extracellular space and body fluids such as urine. Circulating RN (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
PEG precipitation + SEC + dUC + Other
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Sample Condition
Control condition
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Size-exclusion chromatography
Total column volume (mL)
23
Sample volume/column (mL)
0.4
Resin type
Sepharose CL-2B
Other
Name other separation method
PEG precipitation
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Protein Concentration
2.3
Characterization: Particle analysis
NTA
Report type
Mode;mean;size range/distribution;D10;D50;D90
Reported size (nm)
106
EV concentration
Yes
Particle yield
see Fig1A
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170047 | 6/8 | Homo sapiens | Cell culture supernatant | dUC (Differential) (ultra)centrifugation |
Soekmadji C | 2017 | 33% | |
Study summaryFull title
All authors
Soekmadji C, Riches JD, Russell PJ, Ruelcke JE, McPherson S, Wang C, Hovens CM, Corcoran NM, Hill MM, Nelson CC
Journal
Oncotarget
Abstract
Proliferation and maintenance of both normal and prostate cancer (PCa) cells is highly regulated by (show more...)
EV-METRIC
33% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DHT
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC + (Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101/ PSA/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
DHT
EV-producing cells
DUCaP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
120
Pelleting: speed (g)
100000
Wash: time (min)
90
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Concentration
1.6
Western Blot
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD9/ TSG101
Not detected EV-associated proteins
PSA
Proteomics database
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution,Mode
Reported size (nm)
120
|
||||||||
EV170047 | 7/8 | Homo sapiens | Cell culture supernatant | dUC (Differential) (ultra)centrifugation |
Soekmadji C | 2017 | 33% | |
Study summaryFull title
All authors
Soekmadji C, Riches JD, Russell PJ, Ruelcke JE, McPherson S, Wang C, Hovens CM, Corcoran NM, Hill MM, Nelson CC
Journal
Oncotarget
Abstract
Proliferation and maintenance of both normal and prostate cancer (PCa) cells is highly regulated by (show more...)
EV-METRIC
33% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Charcoal stripped serum
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC + (Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101/ PSA/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Charcoal stripped serum
EV-producing cells
DUCaP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
120
Pelleting: speed (g)
100000
Wash: time (min)
90
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Concentration
1.2
Western Blot
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD9/ TSG101
Not detected EV-associated proteins
PSA
Proteomics database
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution,Mode
Reported size (nm)
120
|
||||||||
EV170047 | 8/8 | Homo sapiens | Cell culture supernatant | dUC (Differential) (ultra)centrifugation |
Soekmadji C | 2017 | 33% | |
Study summaryFull title
All authors
Soekmadji C, Riches JD, Russell PJ, Ruelcke JE, McPherson S, Wang C, Hovens CM, Corcoran NM, Hill MM, Nelson CC
Journal
Oncotarget
Abstract
Proliferation and maintenance of both normal and prostate cancer (PCa) cells is highly regulated by (show more...)
EV-METRIC
33% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC + (Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101/ PSA/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
DUCaP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
120
Pelleting: speed (g)
100000
Wash: time (min)
90
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Concentration
1.1
Western Blot
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD9/ TSG101
Not detected EV-associated proteins
PSA
Characterization: Particle analysis
TRPS
Report type
Size range/distribution,Mode
Reported size (nm)
140
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV180003 | 2/3 | Homo sapiens | Cell culture supernatant | dUC | André-Grégoire G | 2017 | 33% | |
Study summaryFull title
All authors
André-Grégoire G, Bidère N, Gavard J
Journal
Biochimie
Abstract
Glioblastoma multiforme (GBM) is the most aggressive primary tumour within the brain as well as the (show more...)
EV-METRIC
33% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Temozolomide-treated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC
Adj. k-factor
255.8 (pelleting) / 255.8 (washing)
Protein markers
EV: Alix/ HSP70
non-EV: TOM20 Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Temozolomide-treated
EV-producing cells
primary glioblastoma cells
EV-harvesting Medium
Serum free medium
Cell viability
90
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
120
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
255.8
Wash: time (min)
120
Wash: Rotor Type
SW 41 Ti
Wash: speed (g)
100000
Wash: adjusted k-factor
255.8
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
Alix, HSP70
Not detected contaminants
TOM20
Proteomics database
No
Characterization: Particle analysis
TRPS
Report type
Mean
Reported size (nm)
90
EV concentration
Yes
|
||||||||
EV170024 | 1/3 | Homo sapiens | Cell culture supernatant | dUC Filtration |
Volgers C | 2017 | 33% | |
Study summaryFull title
All authors
Volgers C, Benedikter BJ, Grauls GE, Savelkoul PHM, Stassen FRM
Journal
Aging (Albany NY)
Abstract
During infection, the release of nano-sized membrane vesicle is a process which is common both for b (show more...)
EV-METRIC
33% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
membrane vesicles
Separation protocol
Separation protocol
dUC + Filtration
Adj. k-factor
156.9 (pelleting)
Protein markers
EV: CD81/ CD63,CD81/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
THP1
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
90
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
156.9
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63,CD81
Flow cytometry specific beads
Selected surface protein(s)
CD63
|
||||||||
EV170024 | 2/3 | Moraxella catarrhalis | Cell culture supernatant | dUC Filtration |
Volgers C | 2017 | 33% | |
Study summaryFull title
All authors
Volgers C, Benedikter BJ, Grauls GE, Savelkoul PHM, Stassen FRM
Journal
Aging (Albany NY)
Abstract
During infection, the release of nano-sized membrane vesicle is a process which is common both for b (show more...)
EV-METRIC
33% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
membrane vesicles
Separation protocol
Separation protocol
dUC + Filtration
Adj. k-factor
156.9 (pelleting)
Protein markers
EV: Moraxella catarrhalis antigen
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Moraxella catarrhalis
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
Moraxella catarrhalis
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
90
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
156.9
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Moraxella catarrhalis antigen
Flow cytometry specific beads
Selected surface protein(s)
Moraxella catarrhalis antigen
Characterization: Particle analysis
TRPS
EV concentration
Yes
Particle yield
1.50E+09 particles/ml start sample
|
||||||||
EV170024 | 3/3 | Pseudomonas aeruginosa | Cell culture supernatant | dUC Filtration |
Volgers C | 2017 | 33% | |
Study summaryFull title
All authors
Volgers C, Benedikter BJ, Grauls GE, Savelkoul PHM, Stassen FRM
Journal
Aging (Albany NY)
Abstract
During infection, the release of nano-sized membrane vesicle is a process which is common both for b (show more...)
EV-METRIC
33% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
membrane vesicles
Separation protocol
Separation protocol
dUC + Filtration
Adj. k-factor
156.9 (pelleting)
Protein markers
EV: Pseudomonas aeruginosa antigen
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Pseudomonas aeruginosa
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
Pseudomonas aeruginosa
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
90
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
156.9
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Pseudomonas aeruginosa antigen
Flow cytometry specific beads
Selected surface protein(s)
Pseudomonas aeruginosa antigen
Characterization: Particle analysis
TRPS
EV concentration
Yes
Particle yield
1.00E+09 particles/ml start sample
|
||||||||
EV170000 | 1/2 | Mus musculus | Serum | dUC Filtration |
Thomou T | 2017 | 29% | |
Study summaryFull title
All authors
Thomou T, Mori MA, Dreyfuss JM, Konishi M, Sakaguchi M, Wolfrum C, Rao TN, Winnay JN, Garcia-Martin R, Grinspoon SK, Gorden P, Kahn
Journal
Nature
Abstract
Adipose tissue is a major site of energy storage and has a role in the regulation of metabolism thro (show more...)
EV-METRIC
29% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NA
Focus vesicles
exosome
Separation protocol
Separation protocol
dUC + Filtration
Protein markers
EV: CD63/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Serum
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
60
Pelleting: speed (g)
100000
Wash: time (min)
60
Wash: speed (g)
100000
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
EM
EM-type
Transmission-EM/ Immune-EM
Proteïns
CD63;CD9
Image type
Close-up, Wide-field
Report size (nm)
80-200
Other particle analysis name(1)
EXOCET ELISA assay
EV-concentration
Yes
|
||||||||
EV170036 | 8/12 | Homo sapiens | Serum | dUC Filtration |
Krafft C | 2017 | 28% | |
Study summaryFull title
All authors
Krafft C, Wilhelm K, Eremin A, Nestel S, von Bubnoff N, Schultze-Seemann W, Popp J, Nazarenko I
Journal
J Cell Sci
Abstract
In cancer, extracellular vesicles (EV) contribute to tumor progression by regulating local and syste (show more...)
EV-METRIC
28% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Benign prostate hyperplasia
Focus vesicles
EV12
Separation protocol
Separation protocol
dUC + Filtration
Adj. k-factor
213.2 (pelleting)
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Sample Condition
Benign prostate hyperplasia
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting: time(min)
90
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
213.2
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
we proceeded with IR and RAMAN analysis of EVs isolated by 12000 x g (frequently designated as micro
Protein Concentration Method
microBCA
Protein Concentration
4-6 for healty donors in EV12; 100 in cancer patients;
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
30-200
Particle yield
1.10E+11 particles/ml start sample
EM
EM-type
Transmission-EM
Image type
Wide-field
Other particle analysis name(1)
Raman spectroscopy
|
||||||||
EV170036 | 10/12 | Homo sapiens | Serum | dUC Filtration |
Krafft C | 2017 | 28% | |
Study summaryFull title
All authors
Krafft C, Wilhelm K, Eremin A, Nestel S, von Bubnoff N, Schultze-Seemann W, Popp J, Nazarenko I
Journal
J Cell Sci
Abstract
In cancer, extracellular vesicles (EV) contribute to tumor progression by regulating local and syste (show more...)
EV-METRIC
28% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Prostate cancer
Focus vesicles
EV120
Separation protocol
Separation protocol
dUC + Filtration
Adj. k-factor
213.2 (pelleting)
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Sample Condition
Prostate cancer
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting: time(min)
90
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
213.2
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
we proceeded with IR and RAMAN analysis of EVs isolated by 12000 x g (frequently designated as micro
Protein Concentration Method
microBCA
Protein Concentration
4-6 for healty donors in EV12; 100 in cancer patients;
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
30-200
Particle yield
1.00E+11 particles/ml start sample
EM
EM-type
Transmission-EM
Image type
Wide-field
Other particle analysis name(1)
Raman spectroscopy
|
||||||||
EV170005 | 1/4 | Homo sapiens | Cell culture supernatant | dUC SEC UF |
Suárez H | 2017 | 28% | |
Study summaryFull title
All authors
Suárez H, Gámez-Valero A, Reyes R, López-Martín S, Rodríguez MJ, Carrascosa JL, Cabañas C, Borràs FE, Yáñez-Mó M
Journal
Sci Rep
Abstract
Most experimental approaches commonly employed for the characterization and quantitation of EVs are (show more...)
EV-METRIC
28% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC + SEC + UF
Protein markers
EV: CD81/ CD59/ CD63/ CD9/ MHC1
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
SK-MEL103
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Ultra filtration
Cut-off size (kDa)
Not spec
Membrane type
Not specified
Size-exclusion chromatography
Total column volume (mL)
20
Sample volume/column (mL)
1.5
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Concentration
148.16
Characterization: Particle analysis
NTA
Report type
Median
|
||||||||
EV170005 | 4/4 | Homo sapiens | Cell culture supernatant | dUC SEC UF |
Suárez H | 2017 | 28% | |
Study summaryFull title
All authors
Suárez H, Gámez-Valero A, Reyes R, López-Martín S, Rodríguez MJ, Carrascosa JL, Cabañas C, Borràs FE, Yáñez-Mó M
Journal
Sci Rep
Abstract
Most experimental approaches commonly employed for the characterization and quantitation of EVs are (show more...)
EV-METRIC
28% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC + SEC + UF
Protein markers
EV: CD81/ CD59/ CD63/ CD9/ MHC1
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
Primary T-lymphoblasts
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Ultra filtration
Cut-off size (kDa)
Not spec
Membrane type
Not specified
Size-exclusion chromatography
Total column volume (mL)
20
Sample volume/column (mL)
1.5
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Concentration
116.85
Characterization: Particle analysis
NTA
Report type
Median
|
||||||||
EV170005 | 3/4 | Homo sapiens | Cell culture supernatant | dUC | Suárez H | 2017 | 25% | |
Study summaryFull title
All authors
Suárez H, Gámez-Valero A, Reyes R, López-Martín S, Rodríguez MJ, Carrascosa JL, Cabañas C, Borràs FE, Yáñez-Mó M
Journal
Sci Rep
Abstract
Most experimental approaches commonly employed for the characterization and quantitation of EVs are (show more...)
EV-METRIC
25% (57th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC
Adj. k-factor
264.9 (pelleting) / 264.9 (washing)
Protein markers
EV: CD59/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
SK-MEL103
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
120
Pelleting: rotor type
AH-627
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
264.9
Wash: time (min)
120
Wash: Rotor Type
AH-627
Wash: speed (g)
100000
Wash: adjusted k-factor
264.9
Characterization: Protein analysis
Protein Concentration Method
BCA
Characterization: Particle analysis
NTA
Report type
Median
|
||||||||
EV170042 | 1/4 | Homo sapiens | Urine | dUC | Silvers CR | 2017 | 22% | |
Study summaryFull title
All authors
Silvers CR, Miyamoto H, Messing EM, Netto GJ, Lee YF
Journal
J Cell Sci
Abstract
The mechanisms of bladder cancer progression are unknown, and new treatments and biomarkers are need (show more...)
EV-METRIC
22% (48th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC
Protein markers
EV: Alix/ Annexin VII/ EHD4/ AnnexinVII
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Urine
Sample Condition
Control condition
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting: time(min)
70
Pelleting: speed (g)
20000
Wash: time (min)
70
Wash: speed (g)
20000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
Alix, Annexin VII, EHD4
|
||||||||
EV170042 | 2/4 | Homo sapiens | Cell culture supernatant | dUC | Silvers CR | 2017 | 22% | |
Study summaryFull title
All authors
Silvers CR, Miyamoto H, Messing EM, Netto GJ, Lee YF
Journal
J Cell Sci
Abstract
The mechanisms of bladder cancer progression are unknown, and new treatments and biomarkers are need (show more...)
EV-METRIC
22% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC
Protein markers
EV: Alix/ TSG101/ CD9
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
TCCSUP
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting: time(min)
70
Pelleting: speed (g)
20000
Wash: time (min)
70
Wash: speed (g)
20000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
Alix, CD9, TSG101
Proteomics database
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
35-300
|
||||||||
EV170042 | 3/4 | Homo sapiens | Urine | dUC | Silvers CR | 2017 | 22% | |
Study summaryFull title
All authors
Silvers CR, Miyamoto H, Messing EM, Netto GJ, Lee YF
Journal
J Cell Sci
Abstract
The mechanisms of bladder cancer progression are unknown, and new treatments and biomarkers are need (show more...)
EV-METRIC
22% (48th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Bladder cancer
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC
Protein markers
EV: SNB1/ EHD4/ Annexin VII/ HEXB/ Alix/ S100A4/ AnnexinVII
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Urine
Sample Condition
Bladder cancer
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting: time(min)
70
Pelleting: speed (g)
20000
Wash: time (min)
70
Wash: speed (g)
20000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
Alix, Annexin VII, EHD4, HEXB, S100A4, SNB1
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV170072 | 1/5 | Homo sapiens | Cell culture supernatant | (Differential) (ultra)centrifugation | Nabet BY | 2017 | 14% | |
Study summaryFull title
All authors
Nabet BY, Qiu Y, Shabason JE, Wu TJ, Yoon T, Kim BC, Benci JL, DeMichele AM, Tchou J, Marcotrigiano J, Minn AJ.
Journal
Cell
Abstract
Interactions between stromal fibroblasts and cancer cells generate signals for cancer progression, t (show more...)
EV-METRIC
14% (39th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
MRC5
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
3h at 100,000g;Other preparation
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
70
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Protein Concentration Method
Lowry-based assay
Characterization: Particle analysis
|
||||||||
EV170072 | 2/5 | Homo sapiens | Cell culture supernatant | (Differential) (ultra)centrifugation | Nabet BY | 2017 | 14% | |
Study summaryFull title
All authors
Nabet BY, Qiu Y, Shabason JE, Wu TJ, Yoon T, Kim BC, Benci JL, DeMichele AM, Tchou J, Marcotrigiano J, Minn AJ.
Journal
Cell
Abstract
Interactions between stromal fibroblasts and cancer cells generate signals for cancer progression, t (show more...)
EV-METRIC
14% (39th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
MDAMB231
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
3h at 100,000g;Other preparation
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
70
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Protein Concentration Method
Lowry-based assay
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
133.4
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV180054 | 1/2 | Homo sapiens | Urine | dUC SEC Ultrafiltration UF |
Lozano-Ramos SI | 2017 | 14% | |
Study summaryFull title
All authors
Lozano-Ramos SI, Bancu I, Carreras-Planella L, Monguió-Tortajada M, Cañas L, Juega J, Bonet J, Armengol MP, Lauzurica R, Borràs FE
Journal
BMC Nephrol
Abstract
BACKGROUND:
Kidney transplantation (KTx) is the best therapeutic approach for chronic kidney disease (show more...)
EV-METRIC
14% (39th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Deceased
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC + SEC + Ultrafiltration + UF
Protein markers
EV: CD63/ CD9
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Urine
Sample Condition
Deceased
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Not specified
Size-exclusion chromatography
Total column volume (mL)
12
Sample volume/column (mL)
0.3
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
Nanodrop
Proteomics database
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
124-250
EV concentration
Yes
|
||||||||
EV180054 | 2/2 | Homo sapiens | Urine | dUC SEC Ultrafiltration UF |
Lozano-Ramos SI | 2017 | 14% | |
Study summaryFull title
All authors
Lozano-Ramos SI, Bancu I, Carreras-Planella L, Monguió-Tortajada M, Cañas L, Juega J, Bonet J, Armengol MP, Lauzurica R, Borràs FE
Journal
BMC Nephrol
Abstract
BACKGROUND:
Kidney transplantation (KTx) is the best therapeutic approach for chronic kidney disease (show more...)
EV-METRIC
14% (39th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC + SEC + Ultrafiltration + UF
Protein markers
EV: CD63/ CD9
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Urine
Sample Condition
Control condition
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Not specified
Size-exclusion chromatography
Total column volume (mL)
12
Sample volume/column (mL)
0.3
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
Nanodrop
Proteomics database
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
124-250
EV concentration
Yes
|
||||||||
EV170042 | 4/4 | Homo sapiens | Cell culture supernatant | dUC | Silvers CR | 2017 | 14% | |
Study summaryFull title
All authors
Silvers CR, Miyamoto H, Messing EM, Netto GJ, Lee YF
Journal
J Cell Sci
Abstract
The mechanisms of bladder cancer progression are unknown, and new treatments and biomarkers are need (show more...)
EV-METRIC
14% (39th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
dUC
Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
SVHUC
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting: time(min)
70
Pelleting: speed (g)
20000
Wash: time (min)
70
Wash: speed (g)
20000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
35-300
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EV170036 | 1/12 | Homo sapiens | Blood plasma | dUC | Krafft C | 2017 | 14% | |
Study summaryFull title
All authors
Krafft C, Wilhelm K, Eremin A, Nestel S, von Bubnoff N, Schultze-Seemann W, Popp J, Nazarenko I
Journal
J Cell Sci
Abstract
In cancer, extracellular vesicles (EV) contribute to tumor progression by regulating local and syste (show more...)
EV-METRIC
14% (45th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Prostate cancer
Focus vesicles
EV12
Separation protocol
Separation protocol
dUC
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Prostate cancer
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting: time(min)
90
Pelleting: speed (g)
12000
Protein Concentration Method
microBCA
Protein Concentration
311
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
30-150
EV concentration
Yes
Particle yield
1.55E+10 particles/ml start sample
EM
EM-type
Transmission-EM
Image type
|