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You searched for: 2016 (Year of publication)
Showing 101 - 150 of 797
Showing 101 - 150 of 797
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220215 | 1/1 | Homo sapiens | Umbilical cord blood-endothelial progenitor cells |
(d)(U)C DC Filtration UF |
Zhang J | 2016 | 33% | |
Study summaryFull title
All authors
Zhang J, Chen C, Hu B, Niu X, Liu X, Zhang G, Zhang C, Li Q, Wang Y
Journal
Int J Biol Sci
Abstract
Chronic skin wounds represent one of the most common and disabling complications of diabetes. Endoth (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Filtration Ultrafiltration Protein markers
EV: CD9/ CD63/ CD81/ CD31
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-endothelial progenitor cells
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Sample volume
0.2
Cushion volume
Not specified
Density of the cushion
30%
Centrifugation time
60
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81/ CD31
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Mode
Reported size (nm)
50-60
EV concentration
Yes
Particle yield
number of particles per million cells
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
50-60
|
||||||||
EV220152 | 1/6 | Homo sapiens | MCF7 | (d)(U)C | Vardaki I | 2016 | 33% | |
Study summaryFull title
All authors
Vardaki I, Ceder S, Rutishauser D, Baltatzis G, Foukakis T, Panaretakis T
Journal
Oncotarget
Abstract
Breast cancer (BrCa) is the most frequent cancer type in women and a leading cause of cancer related (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: beta-catenin/ integrin alpha2/ integrin beta1/ periostin/ E-cadherin/ N-cadherin/ CD81/ TSG101/ ERalpha/ p53/ Glypican-1
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF7
EV-harvesting Medium
EV-depleted medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
120000
Wash: time (min)
120
Wash: speed (g)
120000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
beta-catenin/ integrin alpha2/ integrin beta1/ periostin/ E-cadherin/ N-cadherin/ CD81/ TSG101/ ERalpha
Not detected EV-associated proteins
p53/ Glypican-1
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
108
EV concentration
Yes
|
||||||||
EV220152 | 2/6 | Homo sapiens | MDAMB231 | (d)(U)C | Vardaki I | 2016 | 33% | |
Study summaryFull title
All authors
Vardaki I, Ceder S, Rutishauser D, Baltatzis G, Foukakis T, Panaretakis T
Journal
Oncotarget
Abstract
Breast cancer (BrCa) is the most frequent cancer type in women and a leading cause of cancer related (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: beta-catenin/ integrin alpha2/ integrin beta1/ periostin/ Glypican-1/ N-cadherin/ CD81/ TSG101/ p53/ ERalpha/ E-cadherin
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MDAMB231
EV-harvesting Medium
EV-depleted medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
120000
Wash: time (min)
120
Wash: speed (g)
120000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
beta-catenin/ integrin alpha2/ integrin beta1/ periostin/ Glypican-1/ N-cadherin/ CD81/ TSG101
Not detected EV-associated proteins
p53/ ERalpha/ E-cadherin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
106
EV concentration
Yes
|
||||||||
EV220098 | 1/4 | Homo sapiens | NCI-H460 | (d)(U)C | Lopes-Rodrigues V | 2016 | 33% | |
Study summaryFull title
All authors
Lopes-Rodrigues V, Di Luca A, Sousa D, Seca H, Meleady P, Henry M, Lima RT, O'Connor R, Vasconcelos MH
Journal
Biochim Biophys Acta
Abstract
Multidrug resistance (MDR) is a serious impediment to cancer treatment, with overexpression of drug (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ Syntenin-1/ Clathrin LCB/ CHMP4/ TSG101/ HSP70/ Actin/ P-gp
non-EV: Cytochrome c Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
NCI-H460
EV-harvesting Medium
EV-depleted medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Wash: time (min)
60
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ Syntenin-1/ Clathrin LCB/ CHMP4/ TSG101/ HSP70/ Actin/ P-gp
Not detected contaminants
Cytochrome c
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD81/ CD63
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
0-10000
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
44
|
||||||||
EV220098 | 2/4 | Homo sapiens | RH460 | (d)(U)C | Lopes-Rodrigues V | 2016 | 33% | |
Study summaryFull title
All authors
Lopes-Rodrigues V, Di Luca A, Sousa D, Seca H, Meleady P, Henry M, Lima RT, O'Connor R, Vasconcelos MH
Journal
Biochim Biophys Acta
Abstract
Multidrug resistance (MDR) is a serious impediment to cancer treatment, with overexpression of drug (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ Syntenin-1/ Clathrin LCB/ CHMP4/ TSG101/ HSP70/ Actin/ P-gp
non-EV: Cytochrome c Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
RH460
EV-harvesting Medium
EV-depleted medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Wash: time (min)
60
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ Syntenin-1/ Clathrin LCB/ CHMP4/ TSG101/ HSP70/ Actin/ P-gp
Not detected contaminants
Cytochrome c
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD63/ CD81
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
0-10000
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
85
|
||||||||
EV220098 | 3/4 | Homo sapiens | K562 | (d)(U)C | Lopes-Rodrigues V | 2016 | 33% | |
Study summaryFull title
All authors
Lopes-Rodrigues V, Di Luca A, Sousa D, Seca H, Meleady P, Henry M, Lima RT, O'Connor R, Vasconcelos MH
Journal
Biochim Biophys Acta
Abstract
Multidrug resistance (MDR) is a serious impediment to cancer treatment, with overexpression of drug (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ Syntenin-1/ Clathrin LCB/ CHMP4/ TSG101/ HSP70/ Actin/ P-gp
non-EV: Cytochrome c Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
K562
EV-harvesting Medium
EV-depleted medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Wash: time (min)
60
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ Syntenin-1/ Clathrin LCB/ CHMP4/ TSG101/ HSP70/ Actin
Not detected EV-associated proteins
P-gp
Not detected contaminants
Cytochrome c
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD63/ CD81
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
0-10000
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
58
|
||||||||
EV220098 | 4/4 | Homo sapiens | K562Dox | (d)(U)C | Lopes-Rodrigues V | 2016 | 33% | |
Study summaryFull title
All authors
Lopes-Rodrigues V, Di Luca A, Sousa D, Seca H, Meleady P, Henry M, Lima RT, O'Connor R, Vasconcelos MH
Journal
Biochim Biophys Acta
Abstract
Multidrug resistance (MDR) is a serious impediment to cancer treatment, with overexpression of drug (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ Syntenin-1/ Clathrin LCB/ CHMP4/ TSG101/ HSP70/ Actin/ P-gp
non-EV: Cytochrome c Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
K562Dox
EV-harvesting Medium
EV-depleted medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Wash: time (min)
60
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ Syntenin-1/ Clathrin LCB/ CHMP4/ TSG101/ HSP70/ Actin/ P-gp
Not detected contaminants
Cytochrome c
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD63/ CD81
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
0-10000
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
125
|
||||||||
EV220066 | 1/3 | Homo sapiens | Nasal lavage fluid |
(d)(U)C Filtration |
Lässer C | 2016 | 33% | |
Study summaryFull title
All authors
Lässer C, O'Neil SE, Shelke GV, Sihlbom C, Hansson SF, Gho YS, Lundbäck B, Lötvall J
Journal
J Transl Med
Abstract
Exosomes are nano-sized extracellular vesicles participating in cell-to-cell communication both in h (show more...)
EV-METRIC
33% (83rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Nasal lavage fluid
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: TSG101/ MHC2/ CD63/ CD9
non-EV: calnexin Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Nasal lavage fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
120000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ TSG101
Not detected contaminants
calnexin
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ MHC2
Flow cytometry specific beads
Antibody details provided?
Yes
Antibody dilution provided?
No
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220049 | 1/5 | Homo sapiens | CSF | (d)(U)C | Stuendl A | 2016 | 33% | |
Study summaryFull title
All authors
Stuendl A, Kunadt M, Kruse N, Bartels C, Moebius W, Danzer KM, Mollenhauer B, Schneider A
Journal
Brain
Abstract
Extracellular α-synuclein has been proposed as a crucial mechanism for induction of pathological ag (show more...)
EV-METRIC
33% (78th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
CSF
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Flotillin2/ alpha-synuclein/ IgG2
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
CSF
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Wash: time (min)
60
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin2
Not detected EV-associated proteins
IgG2
Not detected contaminants
Calnexin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
alpha-synuclein
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
40-120
|
||||||||
EV220029 | 1/3 | Homo sapiens | PC-3 | (d)(U)C | Rauschenberger L | 2016 | 33% | |
Study summaryFull title
All authors
Rauschenberger L, Staar D, Thom K, Scharf C, Venz S, Homuth G, Schlüter R, Brandenburg LO, Ziegler P, Zimmermann U, Weitschies W, Völker U, Lendeckel U, Walther R, Burchardt M, Stope MB
Journal
Prostate
Abstract
Remodeling of the tumor environment and the modulation of tumor associated non-malignant cells are e (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: HSP70/ GAPDH/ Actin/ HSP90
non-EV: None Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
PC-3
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
GAPDH/ Actin/ HSP90/ HSP70
Proteomics database
No
Characterization: RNA analysis
RNA analysis
Type
Microarray
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Modus
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV220029 | 2/3 | Homo sapiens | LNCaP | (d)(U)C | Rauschenberger L | 2016 | 33% | |
Study summaryFull title
All authors
Rauschenberger L, Staar D, Thom K, Scharf C, Venz S, Homuth G, Schlüter R, Brandenburg LO, Ziegler P, Zimmermann U, Weitschies W, Völker U, Lendeckel U, Walther R, Burchardt M, Stope MB
Journal
Prostate
Abstract
Remodeling of the tumor environment and the modulation of tumor associated non-malignant cells are e (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: HSP70/ GAPDH/ Actin/ HSP90
non-EV: None Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
LNCaP
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
HSP90/ GAPDH/ Actin/ HSP70
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Modus
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV220029 | 3/3 | Homo sapiens | PC-3-Hsp27 | (d)(U)C | Rauschenberger L | 2016 | 33% | |
Study summaryFull title
All authors
Rauschenberger L, Staar D, Thom K, Scharf C, Venz S, Homuth G, Schlüter R, Brandenburg LO, Ziegler P, Zimmermann U, Weitschies W, Völker U, Lendeckel U, Walther R, Burchardt M, Stope MB
Journal
Prostate
Abstract
Remodeling of the tumor environment and the modulation of tumor associated non-malignant cells are e (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: HSP70/ GAPDH/ Actin/ HSP90
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
PC-3-Hsp27
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
HSP90/ HSP70/ GAPDH/ Actin
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Modus
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV220020 | 1/2 | Homo sapiens | Bone marrow derived mesenchymal stromal cells | (d)(U)C | Fischer S | 2016 | 33% | |
Study summaryFull title
All authors
Fischer S, Cornils K, Speiseder T, Badbaran A, Reimer R, Indenbirken D, Grundhoff A, Brunswig-Spickenheier B, Alawi M, Lange C
Journal
PLoS One
Abstract
The biological relevance of extracellular vesicles (EV) in intercellular communication has been well (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ HSP70/ CD63/ CD9/ GAPDH
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Bone marrow derived mesenchymal stromal cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ GAPDH/ HSP70/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
146
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
FACSAriaIIIu (Becton Dickinson)
Hardware adjustment
Calibration bead size
0.2-0.5-0.75-1-2-3
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
50-150
|
||||||||
EV220015 | 2/9 | Homo sapiens | HepG2 | (d)(U)C | Verma VK | 2016 | 33% | |
Study summaryFull title
All authors
Verma VK, Li H, Wang R, Hirsova P, Mushref M, Liu Y, Cao S, Contreras PC, Malhi H, Kamath PS, Gores GJ, Shah VH
Journal
J Hepatol
Abstract
The mechanisms by which hepatocyte exposure to alcohol activates inflammatory cells such as macropha (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Cytochrome P450 2E1 overexpression
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: LAMP-1/ RAB5/ CD40L/ TSG101/ CD63/ CD40/ IFN-gamma/ IL-23/ IL1-Ra/ PAI-1/ MIF/ IL-16/ MIP-1alpha/ GROalpha/ C5/5a/ I-309/ GM-CSF/ G-CSF/ sICAM-1/ IL-17e/ TNF-alpha/ I-TAC/ IL-13/ RANTES/ IL1-alpha/ sTREM-1/ MCP1/ IL-2/ IL-4/ MIP-1beta/ IL-27/ IL-17/ IL-12p70/ IL-6/ IL-
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HepG2
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
2h at 100,000g/ Other preparation
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: time (min)
120
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
110000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ LAMP-1/ RAB5/ CD40L/ TSG101
Detected EV-associated proteins
CD40/ IFN-gamma/ IL-23/ IL1-Ra/ PAI-1/ MIF/ IL-16/ MIP-1alpha/ GROalpha/ C5/5a/ I-309/ GM-CSF/ G-CSF/ sICAM-1/ IL-17e/ TNF-alpha/ I-TAC/ IL-13/ RANTES/ IL1-alpha/ sTREM-1/ MCP1/
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
110
EV concentration
Yes
EM
EM-type
Immuno-EM
EM protein
Other/ TSG101/ CD40L
Image type
Close-up
|
||||||||
EV210490 | 1/5 | Homo sapiens | Jurkat | (d)(U)C | Bosque A | 2016 | 33% | |
Study summaryFull title
All authors
Bosque A, Dietz L, Gallego-Lleyda A, Sanclemente M, Iturralde M, Naval J, Alava MA, Martínez-Lostao L, Thierse HJ, Anel A
Journal
Oncotarget
Abstract
We have previously characterized that FasL and Apo2L/TRAIL are stored in their bioactive form inside (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: VCP/ CD63
non-EV: None Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Jurkat
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
110000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ VCP
Proteomics database
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
None
|
||||||||
EV210490 | 3/5 | Homo sapiens | T-cell blasts | (d)(U)C | Bosque A | 2016 | 33% | |
Study summaryFull title
All authors
Bosque A, Dietz L, Gallego-Lleyda A, Sanclemente M, Iturralde M, Naval J, Alava MA, Martínez-Lostao L, Thierse HJ, Anel A
Journal
Oncotarget
Abstract
We have previously characterized that FasL and Apo2L/TRAIL are stored in their bioactive form inside (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: VCP
non-EV: None Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
T-cell blasts
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
110000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
VCP
Proteomics database
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
None
|
||||||||
EV210444 | 2/2 | Homo sapiens | Pleural effusion |
(d)(U)C Filtration |
San Lucas FA | 2016 | 33% | |
Study summaryFull title
All authors
San Lucas FA, Allenson K, Bernard V, Castillo J, Kim DU, Ellis K, Ehli EA, Davies GE, Petersen JL, Li D, Wolff R, Katz M, Varadhachary G, Wistuba I, Maitra A, Alvarez H
Journal
Ann Oncol
Abstract
The ability to perform comprehensive profiling of cancers at high resolution is essential for precis (show more...)
EV-METRIC
33% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Pleural effusion
Sample origin
Pancreaticobiliary cancer
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD81/ HSP70/ CD63/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Pleural effusion
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
154000
Wash: volume per pellet (ml)
70
Wash: time (min)
120
Wash: speed (g)
154000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ HSP70/ CD81
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR/ RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
|
||||||||
EV210322 | 4/5 | Homo sapiens | Serum |
(d)(U)C Total Exosome Isolation |
Kibria G | 2016 | 33% | |
Study summaryFull title
All authors
Kibria G, Ramos EK, Lee KE, Bedoyan S, Huang S, Samaeekia R, Athman JJ, Harding CV, Lötvall J, Harris L, Thompson CL, Liu H
Journal
Sci Rep
Abstract
Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human di (show more...)
EV-METRIC
33% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Total Exosome Isolation Protein markers
EV: CD63/ CD81/ LAMP2B/ beta-actin/ CD47/ CD44
non-EV: Grp94 Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
TLA 50.1
Pelleting: speed (g)
100000
Commercial kit
Total Exosome Isolation
Other
Name other separation method
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ CD81/ LAMP2B/ beta-actin
Detected contaminants
Grp94
ELISA
Antibody details provided?
Yes
Detected EV-associated proteins
CD47
Flow cytometry
Type of Flow cytometry
Apogee A50 Micro Flow Cytometer
Calibration bead size
0.11
Antibody details provided?
Yes
Detected EV-associated proteins
CD47/ CD44
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
50-100
|
||||||||
EV210320 | 2/2 | Homo sapiens | HEK293T |
(d)(U)C UF |
Lamichhane TN | 2016 | 33% | |
Study summaryFull title
All authors
Lamichhane TN, Jeyaram A, Patel DB, Parajuli B, Livingston NK, Arumugasaamy N, Schardt JS, Jay SM
Journal
Cell Mol Bioeng
Abstract
Extracellular vesicles (EVs), including exosomes and microvesicles, have emerged as promising drug d (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration Protein markers
EV: Alix/ TSG101
non-EV: GAPDH Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HEK293T
EV-harvesting Medium
EV-depleted medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Ultra filtration
Cut-off size (kDa)
300
Membrane type
NS
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
Alix/ TSG101
Detected contaminants
GAPDH
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
134
EV concentration
Yes
|
||||||||
EV210265 | 1/2 | Homo sapiens | Umbilical cord-derived mesenchymal stem cells |
(d)(U)C Filtration |
Fang S | 2016 | 33% | |
Study summaryFull title
All authors
Fang S, Xu C, Zhang Y, Xue C, Yang C, Bi H, Qian X, Wu M, Ji K, Zhao Y, Wang Y, Liu H, Xing X
Journal
Stem Cells Transl Med
Abstract
: Excessive scar formation caused by myofibroblast aggregations is of great clinical importance duri (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD81/ CD63
non-EV: GAPDH Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord-derived mesenchymal stem cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
3h at 120000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
120000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
120000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81
Detected contaminants
GAPDH
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR/ RNA sequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
|
||||||||
EV210106 | 5/5 | Homo sapiens | Urine |
(d)(U)C Filtration |
Andreu, Zoraida | 2016 | 33% | |
Study summaryFull title
All authors
Zoraida Andreu, Renan Otta Oshiro, Alberto Redruello, Soraya López-Martín, Cristina Gutiérrez-Vázquez, Esperanza Morato, Ana Isabel Marina, Carlos Olivier Gómez, María Yáñez-Mó
Journal
Eur J Pharm Sci.
Abstract
Bladder cancer is the second most frequent malignancy of the urinary tract after prostate cancer. Cu (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Bladder cancer
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: Filamin-A/ ApoE/ ApoB/ CD9/ ERM
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
JS-24.38
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
33
Wash: time (min)
60
Wash: Rotor Type
JS-24.38
Wash: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
ERM/ ApoB/ CD9
Proteomics database
No
Detected EV-associated proteins
Filamin-A/ ApoE/ ApoB
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
130
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV210103 | 4/4 | Homo sapiens | Urine | (d)(U)C | Lin, Shih-Yi | 2016 | 33% | |
Study summaryFull title
All authors
Shih-Yi Lin, Chao-Hsiang Chang, His-Chin Wu, Ching-Chan Lin, Kai-Po Chang, Chi-Rei Yang, Chi-Ping Huang, Wu-Huei Hsu, Chiz-Tzung Chang, Chao-Jung Chen
Journal
Sci Rep
Abstract
MALDI-TOF spectrometry has not been used for urinary exosome analysis. We used it for determining UC (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Urothelial carcinoma
Focus vesicles
microparticle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ TSG101/ actin
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
200000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ actin/ TSG101
Proteomics database
No
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV200178 | 1/4 | Homo sapiens | Blood plasma |
(d)(U)C DC Filtration |
Pillay, Preenan | 2016 | 33% | |
Study summaryFull title
All authors
Preenan Pillay, Niren Maharaj, Jagidesa Moodley, Irene Mackraj
Journal
Placenta
Abstract
Introduction and aim: Exosomes are a subtype of extracellular vesicle (20-130 nm) released by biolog (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Normal pregnancy (< 33 weeks gestation)
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Filtration Protein markers
EV: PLAP/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-55
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
MLA-55
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
Lowry
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
CD63
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ PLAP
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
102.9 + - 12.16
EV concentration
Yes
|
||||||||
EV200178 | 3/4 | Homo sapiens | Blood plasma |
(d)(U)C DC Filtration |
Pillay, Preenan | 2016 | 33% | |
Study summaryFull title
All authors
Preenan Pillay, Niren Maharaj, Jagidesa Moodley, Irene Mackraj
Journal
Placenta
Abstract
Introduction and aim: Exosomes are a subtype of extracellular vesicle (20-130 nm) released by biolog (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Early-onset pre-eclampsia (< 33 weeks gestation)
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Filtration Protein markers
EV: PLAP/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-55
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
MLA-55
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
Lowry
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
CD63
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ PLAP
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
101.8 + - 7.68 nm
EV concentration
Yes
|
||||||||
EV200178 | 4/4 | Homo sapiens | Blood plasma |
(d)(U)C DC Filtration |
Pillay, Preenan | 2016 | 33% | |
Study summaryFull title
All authors
Preenan Pillay, Niren Maharaj, Jagidesa Moodley, Irene Mackraj
Journal
Placenta
Abstract
Introduction and aim: Exosomes are a subtype of extracellular vesicle (20-130 nm) released by biolog (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Late-onset pre-eclampsia (> 34 weeks gestation)
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Filtration Protein markers
EV: PLAP/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-55
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
MLA-55
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
Lowry
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
CD63
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ PLAP
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
104.1 + - 7.65 nm
EV concentration
Yes
|
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