Search > Results
You searched for: EV210003 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210003 | 1/3 | Homo sapiens | PC3 |
(d)(U)C DC |
Qinyu, Ma | 2021 | 44% | |
Study summaryFull title
All authors
Qinyu Ma, Mengmeng Liang, Yutong Wu, Ce Dou, Jianzhong Xu, Shiwu Dong, Fei Luo
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play critical roles in regulating bone metastatic microenvironment thro (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
prostate cancer cell line
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: TSG101/ Alix/ CD63/ CD9/ CD81
non-EV: Argonaute2/ Histone 3/ LaminA/C Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
PC3
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Cell viability (%)
95
Cell count
1,00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
39
Wash: time (min)
70
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
110000
Density cushion
Density medium
Sucrose
Sample volume
30
Cushion volume
9
Density of the cushion
1,21
Centrifugation time
120
Centrifugation speed
110000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ TSG101/ Alix/ CD81
Not detected contaminants
LaminA/C/ Histone 3/ Argonaute2
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
70-140
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 418000
|
||||||||
EV210003 | 2/3 | Homo sapiens | C4 |
(d)(U)C DC |
Qinyu, Ma | 2021 | 44% | |
Study summaryFull title
All authors
Qinyu Ma, Mengmeng Liang, Yutong Wu, Ce Dou, Jianzhong Xu, Shiwu Dong, Fei Luo
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play critical roles in regulating bone metastatic microenvironment thro (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
prostate cancer cell line
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: TSG101/ Alix/ CD63/ CD9/ CD81
non-EV: Argonaute2/ Histone 3/ LaminA/C Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
C4
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Cell viability (%)
96
Cell count
1,00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
39
Wash: time (min)
70
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
110000
Density cushion
Density medium
Sucrose
Sample volume
30
Cushion volume
9
Density of the cushion
1,21
Centrifugation time
120
Centrifugation speed
110000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ TSG101/ Alix/ CD81
Not detected contaminants
LaminA/C/ Histone 3/ Argonaute2
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
70-150
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 461000
|
||||||||
EV210003 | 3/3 | Homo sapiens | C4-2 |
(d)(U)C DC |
Qinyu, Ma | 2021 | 44% | |
Study summaryFull title
All authors
Qinyu Ma, Mengmeng Liang, Yutong Wu, Ce Dou, Jianzhong Xu, Shiwu Dong, Fei Luo
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play critical roles in regulating bone metastatic microenvironment thro (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
prostate cancer cell line
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: Alix/ TSG101/ CD63/ CD9/ CD81
non-EV: Argonaute2/ Histone 3/ LaminA/C Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
C4-2
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Cell viability (%)
96
Cell count
1,00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
39
Wash: time (min)
70
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
110000
Density cushion
Density medium
Sucrose
Sample volume
30
Cushion volume
9
Density of the cushion
1,21
Centrifugation time
120
Centrifugation speed
110000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101/ CD81
Not detected contaminants
LaminA/C/ Histone 3/ Argonaute2
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
70-150
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 445000
|
||||||||
1 - 3 of 3 |
EV-TRACK ID | EV210003 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | ||
cell type | PC3 | C4 | C4-2 |
condition | prostate cancer cell line | prostate cancer cell line | prostate cancer cell line |
separation protocol | dUC DC | dUC DC | dUC DC |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 44 | 44 | 44 |