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You searched for: EV200178 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200178 | 2/4 | Homo sapiens | Blood plasma | (Differential) (ultra)centrifugation Density cushion Filtration |
Pillay, Preenan | 2016 | 44% | |
Study summaryFull title
All authors
Preenan Pillay, Niren Maharaj, Jagidesa Moodley, Irene Mackraj
Journal
Placenta
Abstract
Introduction and aim: Exosomes are a subtype of extracellular vesicle (20-130 nm) released by biolog (show more...)
EV-METRIC
44% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Normal pregnancy (>34 weeks gestation)
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation + Density cushion + Filtration
Protein markers
EV: PLAP/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Normal pregnancy (>34 weeks gestation)
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-55
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
MLA-55
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
Lowry
Western Blot
Detected EV-associated proteins
CD63
ELISA
Detected EV-associated proteins
CD63/ PLAP
Flow cytometry
Hardware adjustments
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
100.3 + - 7.78 nm
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
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EV200178 | 1/4 | Homo sapiens | Blood plasma | (Differential) (ultra)centrifugation Density cushion Filtration |
Pillay, Preenan | 2016 | 33% | |
Study summaryFull title
All authors
Preenan Pillay, Niren Maharaj, Jagidesa Moodley, Irene Mackraj
Journal
Placenta
Abstract
Introduction and aim: Exosomes are a subtype of extracellular vesicle (20-130 nm) released by biolog (show more...)
EV-METRIC
33% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Normal pregnancy (< 33 weeks gestation)
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation + Density cushion + Filtration
Protein markers
EV: PLAP/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Normal pregnancy (< 33 weeks gestation)
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-55
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
MLA-55
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
Lowry
Western Blot
Detected EV-associated proteins
CD63
ELISA
Detected EV-associated proteins
CD63/ PLAP
Flow cytometry
Hardware adjustments
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
102.9 + - 12.16
EV concentration
Yes
|
||||||||
EV200178 | 3/4 | Homo sapiens | Blood plasma | (Differential) (ultra)centrifugation Density cushion Filtration |
Pillay, Preenan | 2016 | 33% | |
Study summaryFull title
All authors
Preenan Pillay, Niren Maharaj, Jagidesa Moodley, Irene Mackraj
Journal
Placenta
Abstract
Introduction and aim: Exosomes are a subtype of extracellular vesicle (20-130 nm) released by biolog (show more...)
EV-METRIC
33% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Early-onset pre-eclampsia (< 33 weeks gestation)
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation + Density cushion + Filtration
Protein markers
EV: PLAP/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Early-onset pre-eclampsia (< 33 weeks gestation)
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-55
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
MLA-55
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
Lowry
Western Blot
Detected EV-associated proteins
CD63
ELISA
Detected EV-associated proteins
CD63/ PLAP
Flow cytometry
Hardware adjustments
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
101.8 + - 7.68 nm
EV concentration
Yes
|
||||||||
EV200178 | 4/4 | Homo sapiens | Blood plasma | (Differential) (ultra)centrifugation Density cushion Filtration |
Pillay, Preenan | 2016 | 33% | |
Study summaryFull title
All authors
Preenan Pillay, Niren Maharaj, Jagidesa Moodley, Irene Mackraj
Journal
Placenta
Abstract
Introduction and aim: Exosomes are a subtype of extracellular vesicle (20-130 nm) released by biolog (show more...)
EV-METRIC
33% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Late-onset pre-eclampsia (> 34 weeks gestation)
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation + Density cushion + Filtration
Protein markers
EV: PLAP/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Late-onset pre-eclampsia (> 34 weeks gestation)
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-55
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
MLA-55
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
Lowry
Western Blot
Detected EV-associated proteins
CD63
ELISA
Detected EV-associated proteins
CD63/ PLAP
Flow cytometry
Hardware adjustments
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
104.1 + - 7.65 nm
EV concentration
Yes
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