Search > Results
You searched for: EV200153 (EV-TRACK ID)
Showing 1 - 6 of 6
Showing 1 - 6 of 6
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200153 | 1/6 | Homo sapiens | Cell culture supernatant | Density gradient (Differential) (ultra)centrifugation Filtration |
Grace Truong | 2017 | 56% | |
Study summaryFull title
All authors
Grace Truong, Dominic Guanzon, Vyjayanthi Kinhal, Omar Elfeky, Andrew Lai, Sherri Longo, Zarin Nuzhat, Carlos Palma, Katherin Scholz-Romero, Ramkumar Menon, Ben W Mol, Gregory E Rice, Carlos Salomon
Journal
PLoS One
Abstract
Our understanding of how cells communicate has undergone a paradigm shift since the recent recogniti (show more...)
EV-METRIC
56% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
8% oxygen
Focus vesicles
exosome
Separation protocol
Separation protocol
Density gradient + (Differential) (ultra)centrifugation + Filtration
Protein markers
EV: TSG101/ CD63
non-EV: None Proteomics
no
EV density (g/ml)
1.13-1.19
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
8% oxygen
EV-producing cells
HTR-8/SVneo
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Not specified
Cell number specification
No
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Surespin 630/36
Pelleting: speed (g)
100000
Density gradient
Density medium
Iodixanol
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
14.5mL
Sample volume (mL)
0.5mL
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
Not specified
Fraction processing
Centrifugation
Pelleting: volume per fraction
Not spec
Pelleting: duration (min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
TSG101
Flow cytometry
Hardware adjustments
Fluorescent NTA
Relevant measurements variables specified?
NA
Detected EV-associated proteins
CD63
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
108+/-15
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
Around 100nm
|
||||||||
EV200153 | 2/6 | Homo sapiens | Cell culture supernatant | Density gradient (Differential) (ultra)centrifugation Filtration |
Grace Truong | 2017 | 45% | |
Study summaryFull title
All authors
Grace Truong, Dominic Guanzon, Vyjayanthi Kinhal, Omar Elfeky, Andrew Lai, Sherri Longo, Zarin Nuzhat, Carlos Palma, Katherin Scholz-Romero, Ramkumar Menon, Ben W Mol, Gregory E Rice, Carlos Salomon
Journal
PLoS One
Abstract
Our understanding of how cells communicate has undergone a paradigm shift since the recent recogniti (show more...)
EV-METRIC
45% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
1% oxygen
Focus vesicles
exosome
Separation protocol
Separation protocol
Density gradient + (Differential) (ultra)centrifugation + Filtration
Protein markers
EV: CD63
non-EV: None Proteomics
no
EV density (g/ml)
1.13-1.19
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
1% oxygen
EV-producing cells
HTR-8/SVneo
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Not specified
Cell number specification
No
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Surespin 630/36
Pelleting: speed (g)
100000
Density gradient
Density medium
Iodixanol
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
14.5mL
Sample volume (mL)
0.5mL
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
Not specified
Fraction processing
Centrifugation
Pelleting: volume per fraction
Not spec
Pelleting: duration (min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Hardware adjustments
Fluorescent NTA
Relevant measurements variables specified?
NA
Detected EV-associated proteins
CD63
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
108+/-15
EV concentration
Yes
|
||||||||
EV200153 | 3/6 | Homo sapiens | Cell culture supernatant | Density gradient (Differential) (ultra)centrifugation Filtration |
Grace Truong | 2017 | 34% | |
Study summaryFull title
All authors
Grace Truong, Dominic Guanzon, Vyjayanthi Kinhal, Omar Elfeky, Andrew Lai, Sherri Longo, Zarin Nuzhat, Carlos Palma, Katherin Scholz-Romero, Ramkumar Menon, Ben W Mol, Gregory E Rice, Carlos Salomon
Journal
PLoS One
Abstract
Our understanding of how cells communicate has undergone a paradigm shift since the recent recogniti (show more...)
EV-METRIC
34% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
HTR-8/SVneo EV treatment
Focus vesicles
exosome
Separation protocol
Separation protocol
Density gradient + (Differential) (ultra)centrifugation + Filtration
Protein markers
EV: TNF-alpha
non-EV: None Proteomics
no
EV density (g/ml)
1.13-1.19
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
HTR-8/SVneo EV treatment
EV-producing cells
HUVEC
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Not specified
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Surespin 630/36
Pelleting: speed (g)
100000
Density gradient
Density medium
Iodixanol
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
14.5mL
Sample volume (mL)
0.5mL
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
Not specified
Fraction processing
Centrifugation
Pelleting: volume per fraction
Not spec
Pelleting: duration (min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
ELISA
Detected EV-associated proteins
TNF-alpha
Flow cytometry
Hardware adjustments
|
||||||||
EV200153 | 4/6 | Homo sapiens | Blood plasma | Density gradient (Differential) (ultra)centrifugation Filtration |
Grace Truong | 2017 | 34% | |
Study summaryFull title
All authors
Grace Truong, Dominic Guanzon, Vyjayanthi Kinhal, Omar Elfeky, Andrew Lai, Sherri Longo, Zarin Nuzhat, Carlos Palma, Katherin Scholz-Romero, Ramkumar Menon, Ben W Mol, Gregory E Rice, Carlos Salomon
Journal
PLoS One
Abstract
Our understanding of how cells communicate has undergone a paradigm shift since the recent recogniti (show more...)
EV-METRIC
34% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Healthy pregnant
Focus vesicles
exosome
Separation protocol
Separation protocol
Density gradient + (Differential) (ultra)centrifugation + Filtration
Protein markers
EV: HLA-G
non-EV: None Proteomics
no
EV density (g/ml)
1.13-1.19
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Healthy pregnant
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Surespin 630/36
Pelleting: speed (g)
100000
Density gradient
Density medium
Iodixanol
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
14.5mL
Sample volume (mL)
0.5mL
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
Not specified
Fraction processing
Centrifugation
Pelleting: volume per fraction
Not spec
Pelleting: duration (min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
HLA-G
Flow cytometry
Hardware adjustments
Characterization: Particle analysis
NTA
Report type
Mean +/- SEM;Other
Reported size (nm)
105+/-15
EV concentration
Yes
|
||||||||
EV200153 | 5/6 | Homo sapiens | Blood plasma | Density gradient (Differential) (ultra)centrifugation Filtration |
Grace Truong | 2017 | 34% | |
Study summaryFull title
All authors
Grace Truong, Dominic Guanzon, Vyjayanthi Kinhal, Omar Elfeky, Andrew Lai, Sherri Longo, Zarin Nuzhat, Carlos Palma, Katherin Scholz-Romero, Ramkumar Menon, Ben W Mol, Gregory E Rice, Carlos Salomon
Journal
PLoS One
Abstract
Our understanding of how cells communicate has undergone a paradigm shift since the recent recogniti (show more...)
EV-METRIC
34% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Pregnant; Pre-eclampsia
Focus vesicles
exosome
Separation protocol
Separation protocol
Density gradient + (Differential) (ultra)centrifugation + Filtration
Protein markers
EV: HLA-G
non-EV: None Proteomics
no
EV density (g/ml)
1.13-1.19
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Pregnant; Pre-eclampsia
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Surespin 630/36
Pelleting: speed (g)
100000
Density gradient
Density medium
Iodixanol
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
14.5mL
Sample volume (mL)
0.5mL
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
Not specified
Fraction processing
Centrifugation
Pelleting: volume per fraction
Not spec
Pelleting: duration (min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
HLA-G
Flow cytometry
Hardware adjustments
Characterization: Particle analysis
NTA
Report type
Mean +/- SEM;Other
Reported size (nm)
95 +/- 25
EV concentration
Yes
|
||||||||
EV200153 | 6/6 | Homo sapiens | Blood plasma | Density gradient (Differential) (ultra)centrifugation Filtration |
Grace Truong | 2017 | 34% | |
Study summaryFull title
All authors
Grace Truong, Dominic Guanzon, Vyjayanthi Kinhal, Omar Elfeky, Andrew Lai, Sherri Longo, Zarin Nuzhat, Carlos Palma, Katherin Scholz-Romero, Ramkumar Menon, Ben W Mol, Gregory E Rice, Carlos Salomon
Journal
PLoS One
Abstract
Our understanding of how cells communicate has undergone a paradigm shift since the recent recogniti (show more...)
EV-METRIC
34% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Pregnant; Spontaneous pre-term birth
Focus vesicles
exosome
Separation protocol
Separation protocol
Density gradient + (Differential) (ultra)centrifugation + Filtration
Protein markers
EV: HLA-G
non-EV: None Proteomics
no
EV density (g/ml)
1.13-1.19
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Pregnant; Spontaneous pre-term birth
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Surespin 630/36
Pelleting: speed (g)
100000
Density gradient
Density medium
Iodixanol
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
14.5mL
Sample volume (mL)
0.5mL
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
Not specified
Fraction processing
Centrifugation
Pelleting: volume per fraction
Not spec
Pelleting: duration (min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
HLA-G
Flow cytometry
Hardware adjustments
Characterization: Particle analysis
NTA
Report type
Mean +/- SEM;Other
Reported size (nm)
102 +/- 22
EV concentration
Yes
|
||||||||
1 - 6 of 6 |