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You searched for: EV200125 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200125 | 1/2 | Homo sapiens | Cell culture supernatant | (d)(U)C | Aminzadeh, Mark | 2021 | 56% | |
Study summaryFull title
All authors
Mark A. Aminzadeh, Mario Fournier, Akbarshakh Akhmerov, K. Candis Jones‐Ungerleider, Jackelyn B. Valle, Eduardo Marbán
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) from cardiac stromal cells, developed as therapeutic candidates, improv (show more...)
EV-METRIC
56% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
cardiosphere-derived cells
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/New methodological development/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
cardiosphere-derived cells
EV-harvesting Medium
Serum free medium
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Flow cytometry
Hardware adjustments
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
150
Used for determining EV concentration?
Yes
EM
EM-type
Immuno-EM/ Transmission-EM
Proteïns
CD63
Image type
Close-up, Wide-field
|
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EV200125 | 2/2 | Homo sapiens | Cell culture supernatant | (d)(U)C | Aminzadeh, Mark | 2021 | 56% | |
Study summaryFull title
All authors
Mark A. Aminzadeh, Mario Fournier, Akbarshakh Akhmerov, K. Candis Jones‐Ungerleider, Jackelyn B. Valle, Eduardo Marbán
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) from cardiac stromal cells, developed as therapeutic candidates, improv (show more...)
EV-METRIC
56% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
cardiosphere-derived cells
Sample origin
Hypoxia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/New methodological development/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Hypoxia
EV-producing cells
cardiosphere-derived cells
EV-harvesting Medium
Serum free medium
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Flow cytometry
Hardware adjustments
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
150
Used for determining EV concentration?
Yes
EM
EM-type
Immuno-EM/ Transmission-EM
Proteïns
CD63
Image type
Close-up, Wide-field
|
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