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Results list Most used separation protocols Top EV-enriched proteins

Details	EV-TRACK ID	Experiment nr.	Species	Sample type	separation protocol	First author	Year	EV-METRIC
	EV200125	1/2	Homo sapiens	Cell culture supernatant	(d)(U)C	Aminzadeh, Mark	2021	56%
Study summary Full title Casein‐enhanced uptake and disease‐modifying bioactivity of ingested extracellular vesicles All authors Mark A. Aminzadeh, Mario Fournier, Akbarshakh Akhmerov, K. Candis Jones‐Ungerleider, Jackelyn B. Valle, Eduardo Marbán Journal J Extracell Vesicles Abstract Extracellular vesicles (EVs) from cardiac stromal cells, developed as therapeutic candidates, improv (show more...)Extracellular vesicles (EVs) from cardiac stromal cells, developed as therapeutic candidates, improve dystrophic muscle function when administered parenterally, but oral delivery remains untested. We find that casein, the dominant protein in breast milk, enhances the uptake and bioactivity of ingested heart‐derived EVs, altering gene expression in blood cells and enhancing muscle function in mdx mice with muscular dystrophy. Thus, EVs, administered orally, are absorbed and exert disease‐modifying bioactivity in vivo. Formulating EVs with casein enhances uptake and markedly expands the range of potential therapeutic applications. (hide) EV-METRIC 56% (85th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Cell Name cardiosphere-derived cells Sample origin Control condition Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. (d)(U)C = (differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography (Differential) (ultra)centrifugation Protein markers EV: None non-EV: None Proteomics no Show all info Study aim Function/New methodological development/Biogenesis/cargo sorting/Mechanism of uptake/transfer Sample Species Homo sapiens Sample Type Cell culture supernatant Sample Condition Control condition EV-producing cells cardiosphere-derived cells EV-harvesting Medium Serum free medium Separation Method Differential ultracentrifugation centrifugation steps Between 800 g and 10,000 g Between 100,000 g and 150,000 g Obtain an EV pellet : Yes Pelleting: time(min) 60 Pelleting: rotor type SW 28 Pelleting: speed (g) 100000 Characterization: Protein analysis Protein Concentration Method microBCA Flow cytometry Hardware adjustments Characterization: Particle analysis DLS Report type Mean Reported size (nm) 150 Used for determining EV concentration? Yes EM EM-type Immuno-EM/ Transmission-EM Proteïns CD63 Image type Close-up, Wide-field

	EV200125	2/2	Homo sapiens	Cell culture supernatant	(d)(U)C	Aminzadeh, Mark	2021	56%
Study summary Full title Casein‐enhanced uptake and disease‐modifying bioactivity of ingested extracellular vesicles All authors Mark A. Aminzadeh, Mario Fournier, Akbarshakh Akhmerov, K. Candis Jones‐Ungerleider, Jackelyn B. Valle, Eduardo Marbán Journal J Extracell Vesicles Abstract Extracellular vesicles (EVs) from cardiac stromal cells, developed as therapeutic candidates, improv (show more...)Extracellular vesicles (EVs) from cardiac stromal cells, developed as therapeutic candidates, improve dystrophic muscle function when administered parenterally, but oral delivery remains untested. We find that casein, the dominant protein in breast milk, enhances the uptake and bioactivity of ingested heart‐derived EVs, altering gene expression in blood cells and enhancing muscle function in mdx mice with muscular dystrophy. Thus, EVs, administered orally, are absorbed and exert disease‐modifying bioactivity in vivo. Formulating EVs with casein enhances uptake and markedly expands the range of potential therapeutic applications. (hide) EV-METRIC 56% (85th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Cell Name cardiosphere-derived cells Sample origin Hypoxia Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. (d)(U)C = (differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography (Differential) (ultra)centrifugation Protein markers EV: None non-EV: None Proteomics no Show all info Study aim Function/New methodological development/Biogenesis/cargo sorting/Mechanism of uptake/transfer Sample Species Homo sapiens Sample Type Cell culture supernatant Sample Condition Hypoxia EV-producing cells cardiosphere-derived cells EV-harvesting Medium Serum free medium Separation Method Differential ultracentrifugation centrifugation steps Between 800 g and 10,000 g Between 100,000 g and 150,000 g Obtain an EV pellet : Yes Pelleting: time(min) 60 Pelleting: rotor type SW 28 Pelleting: speed (g) 100000 Characterization: Protein analysis Protein Concentration Method microBCA Flow cytometry Hardware adjustments Characterization: Particle analysis DLS Report type Mean Reported size (nm) 150 Used for determining EV concentration? Yes EM EM-type Immuno-EM/ Transmission-EM Proteïns CD63 Image type Close-up, Wide-field

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