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You searched for: EV200114 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200114 | 1/4 | Homo sapiens | Cell culture supernatant | Density gradient (Differential) (ultra)centrifugation Filtration |
Omar Elfeky | 2017 | 50% | |
Study summaryFull title
All authors
Omar Elfeky, Sherri Longo, Andrew Lai, Gregory E Rice, Carlos Salomon
Journal
Placenta
Abstract
Recent studies report that 35% of women are either overweight or obese at reproductive age. The plac (show more...)
EV-METRIC
50% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
Density gradient + (Differential) (ultra)centrifugation + Filtration
Protein markers
EV: CD63/ PLAP/ IgG1
non-EV: None Proteomics
no
EV density (g/ml)
1.12-1.19
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
Trophoblasts
EV-harvesting Medium
Not specified
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 100,000 g and 150,000 g
Obtain an EV pellet :
Yes
Pelleting: time(min)
1200
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Density medium
Iodixanol
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Sample volume (mL)
0.5mL
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1200
Fraction volume (mL)
Not specified
Fraction processing
Not specified
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Fluorescent NTA
Relevant measurements variables specified?
NA
Detected EV-associated proteins
PLAP/ IgG1/ CD63
Characterization: Particle analysis
DLS
Report type
Not Reported
Used for determining EV concentration?
Yes
NTA
Report type
Not Reported
EV concentration
Yes
|
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EV200114 | 3/4 | Homo sapiens | Blood plasma | Density gradient (Differential) (ultra)centrifugation No extra separation steps |
Omar Elfeky | 2017 | 50% | |
Study summaryFull title
All authors
Omar Elfeky, Sherri Longo, Andrew Lai, Gregory E Rice, Carlos Salomon
Journal
Placenta
Abstract
Recent studies report that 35% of women are either overweight or obese at reproductive age. The plac (show more...)
EV-METRIC
50% (83rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Overweight (BMI 25-29.9kg/m2)
Focus vesicles
exosome
Separation protocol
Separation protocol
Density gradient + (Differential) (ultra)centrifugation + No extra separation steps
Protein markers
EV: CD63/ PLAP/ IgG1
non-EV: None Proteomics
no
EV density (g/ml)
1.12-1.19
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Overweight (BMI 25-29.9kg/m2)
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 100,000 g and 150,000 g
Obtain an EV pellet :
Yes
Pelleting: time(min)
1200
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Density medium
Iodixanol
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Sample volume (mL)
0.5mL
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1200
Fraction volume (mL)
Not specified
Fraction processing
Not specified
Characterization: Protein analysis
Protein Concentration Method
Not determined
Fluorescent NTA
Relevant measurements variables specified?
NA
Detected EV-associated proteins
PLAP/ IgG1/ CD63
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
98
Used for determining EV concentration?
Yes
NTA
Report type
Not Reported
EV concentration
Yes
|
||||||||
EV200114 | 4/4 | Homo sapiens | Blood plasma | Density gradient (Differential) (ultra)centrifugation No extra separation steps |
Omar Elfeky | 2017 | 50% | |
Study summaryFull title
All authors
Omar Elfeky, Sherri Longo, Andrew Lai, Gregory E Rice, Carlos Salomon
Journal
Placenta
Abstract
Recent studies report that 35% of women are either overweight or obese at reproductive age. The plac (show more...)
EV-METRIC
50% (83rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Obese (BMI >= 30kg/m2)
Focus vesicles
exosome
Separation protocol
Separation protocol
Density gradient + (Differential) (ultra)centrifugation + No extra separation steps
Protein markers
EV: CD63/ PLAP/ IgG1
non-EV: None Proteomics
no
EV density (g/ml)
1.12-1.19
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Obese (BMI >= 30kg/m2)
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 100,000 g and 150,000 g
Obtain an EV pellet :
Yes
Pelleting: time(min)
1200
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Density medium
Iodixanol
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Sample volume (mL)
0.5mL
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1200
Fraction volume (mL)
Not specified
Fraction processing
Not specified
Characterization: Protein analysis
Protein Concentration Method
Not determined
Fluorescent NTA
Relevant measurements variables specified?
NA
Detected EV-associated proteins
PLAP/ IgG1/ CD63
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
105
Used for determining EV concentration?
Yes
NTA
Report type
Not Reported
EV concentration
Yes
|
||||||||
EV200114 | 2/4 | Homo sapiens | Blood plasma | Density gradient (Differential) (ultra)centrifugation No extra separation steps |
Omar Elfeky | 2017 | 44% | |
Study summaryFull title
All authors
Omar Elfeky, Sherri Longo, Andrew Lai, Gregory E Rice, Carlos Salomon
Journal
Placenta
Abstract
Recent studies report that 35% of women are either overweight or obese at reproductive age. The plac (show more...)
EV-METRIC
44% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Healthy pregnant
Focus vesicles
exosome
Separation protocol
Separation protocol
Density gradient + (Differential) (ultra)centrifugation + No extra separation steps
Protein markers
EV: CD63/ PLAP/ IgG1
non-EV: None Proteomics
no
EV density (g/ml)
1.12-1.19
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Healthy pregnant
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 100,000 g and 150,000 g
Obtain an EV pellet :
Yes
Pelleting: time(min)
1200
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Density medium
Iodixanol
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Sample volume (mL)
0.5mL
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1200
Fraction volume (mL)
Not specified
Fraction processing
Not specified
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
CD63
Fluorescent NTA
Relevant measurements variables specified?
NA
Detected EV-associated proteins
PLAP/ IgG1/ CD63
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
102
Used for determining EV concentration?
Yes
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
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