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You searched for: EV200085 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200085 | 1/5 | Rattus norvegicus | Cell culture supernatant | DG (d)(U)C |
Rohit Kumar | 2020 | 45% | |
Study summaryFull title
All authors
Rohit Kumar, Qilin Tang, Stephan A Müller, Pan Gao, Diana Mahlstedt, Sofia Zampagni, Yi Tan, Andreas Klingl, Kai Bötzel, Stefan F Lichtenthaler, Günter U Höglinger, Thomas Koeglsperger
Journal
Advanced Science
Abstract
Extracellular vesicles (EVs) are endogenous membrane-derived vesicles that shuttle bioactive molecul (show more...)
EV-METRIC
45% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
Primary Hippocampus neurons
Sample origin
CD63-pHluorin transduced
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: Alix/ CD81/ Flotillin1/ CD9/ GFP/ VAMP3/ VAMP2
non-EV: None Proteomics
yes
EV density (g/ml)
1.08-1.14
Show all info
Study aim
Function/Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Rattus norvegicus
Sample Type
Cell culture supernatant
Sample Condition
CD63-pHluorin transduced
EV-producing cells
Primary Hippocampus neurons
EV-harvesting Medium
Serum free
Cell viability
Yes
Cell number specification
Yes
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
MLA-80
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
1
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Density medium
Iodixanol
Type
Continuous
Lowest density fraction
10%
Highest density fraction
30%
Total gradient volume, incl. sample (mL)
5.5
Sample volume (mL)
3
Orientation
Bottom-up
Rotor type
SW 55 Ti
Speed (g)
350000
Duration (min)
60
Fraction volume (mL)
0.49
Fraction processing
Centrifugation
Pelleting: volume per fraction
3
Pelleting: duration (min)
30
Pelleting: rotor type
TLA-110
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Alix/ CD81/ Flotillin1/ CD9/ GFP/ VAMP3
Not detected EV-associated proteins
VAMP2
Proteomics
Proteomics database
Yes
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
0-500
EV concentration
Yes
|
||||||||
EV200085 | 2/5 | Rattus norvegicus | Cell culture supernatant | DG (d)(U)C |
Rohit Kumar | 2020 | 23% | |
Study summaryFull title
All authors
Rohit Kumar, Qilin Tang, Stephan A Müller, Pan Gao, Diana Mahlstedt, Sofia Zampagni, Yi Tan, Andreas Klingl, Kai Bötzel, Stefan F Lichtenthaler, Günter U Höglinger, Thomas Koeglsperger
Journal
Advanced Science
Abstract
Extracellular vesicles (EVs) are endogenous membrane-derived vesicles that shuttle bioactive molecul (show more...)
EV-METRIC
23% (49th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
Primary Hippocampus neurons
Sample origin
CD63-pHluorin transduced + bFGF treated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: Alix/ CD81/ Flotillin1/ CD9/ GFP/ VAMP3
non-EV: VAMP2 Proteomics
yes
Show all info
Study aim
Function/Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Rattus norvegicus
Sample Type
Cell culture supernatant
Sample Condition
CD63-pHluorin transduced + bFGF treated
EV-producing cells
Primary Hippocampus neurons
EV-harvesting Medium
Serum free
Cell viability
Yes
Cell number specification
Yes
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
MLA-80
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
1
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Alix/ CD81/ Flotillin1/ CD9/ GFP/ VAMP3
Not detected EV-associated proteins
VAMP2
Proteomics
Proteomics database
Yes
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
0-500
EV concentration
Yes
|
||||||||
EV200085 | 3/5 | Rattus norvegicus | Cell culture supernatant | DG (d)(U)C |
Rohit Kumar | 2020 | 23% | |
Study summaryFull title
All authors
Rohit Kumar, Qilin Tang, Stephan A Müller, Pan Gao, Diana Mahlstedt, Sofia Zampagni, Yi Tan, Andreas Klingl, Kai Bötzel, Stefan F Lichtenthaler, Günter U Höglinger, Thomas Koeglsperger
Journal
Advanced Science
Abstract
Extracellular vesicles (EVs) are endogenous membrane-derived vesicles that shuttle bioactive molecul (show more...)
EV-METRIC
23% (49th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
Primary Hippocampus neurons
Sample origin
CD63-pHluorin transduced + siRNA VAMP3 + bFGF treated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: Alix/ CD81/ Flotillin1/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Rattus norvegicus
Sample Type
Cell culture supernatant
Sample Condition
CD63-pHluorin transduced + siRNA VAMP3 + bFGF treated
EV-producing cells
Primary Hippocampus neurons
EV-harvesting Medium
Serum free
Cell viability
Yes
Cell number specification
Yes
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
MLA-80
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
1
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Alix/ CD81/ Flotillin1/ CD9
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
0-500
EV concentration
Yes
|
||||||||
EV200085 | 4/5 | Rattus norvegicus | Cell culture supernatant | DG (d)(U)C |
Rohit Kumar | 2020 | 23% | |
Study summaryFull title
All authors
Rohit Kumar, Qilin Tang, Stephan A Müller, Pan Gao, Diana Mahlstedt, Sofia Zampagni, Yi Tan, Andreas Klingl, Kai Bötzel, Stefan F Lichtenthaler, Günter U Höglinger, Thomas Koeglsperger
Journal
Advanced Science
Abstract
Extracellular vesicles (EVs) are endogenous membrane-derived vesicles that shuttle bioactive molecul (show more...)
EV-METRIC
23% (49th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
Primary Hippocampus neurons
Sample origin
CD63-pHluorin transduced + bFGF and BABPTA-AM treated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: Alix/ CD81/ GFP
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Rattus norvegicus
Sample Type
Cell culture supernatant
Sample Condition
CD63-pHluorin transduced + bFGF and BABPTA-AM treated
EV-producing cells
Primary Hippocampus neurons
EV-harvesting Medium
Serum free
Cell viability
Yes
Cell number specification
Yes
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
MLA-80
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
1
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Alix/ CD81/ GFP
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
0-500
EV concentration
Yes
|
||||||||
EV200085 | 5/5 | Rattus norvegicus | Cell culture supernatant | DG (d)(U)C |
Rohit Kumar | 2020 | 15% | |
Study summaryFull title
All authors
Rohit Kumar, Qilin Tang, Stephan A Müller, Pan Gao, Diana Mahlstedt, Sofia Zampagni, Yi Tan, Andreas Klingl, Kai Bötzel, Stefan F Lichtenthaler, Günter U Höglinger, Thomas Koeglsperger
Journal
Advanced Science
Abstract
Extracellular vesicles (EVs) are endogenous membrane-derived vesicles that shuttle bioactive molecul (show more...)
EV-METRIC
15% (39th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
Primary Hippocampus neurons
Sample origin
CD63-pHluorin transduced + bFGF and Genistein treated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Rattus norvegicus
Sample Type
Cell culture supernatant
Sample Condition
CD63-pHluorin transduced + bFGF and Genistein treated
EV-producing cells
Primary Hippocampus neurons
EV-harvesting Medium
Serum free
Cell viability
Yes
Cell number specification
Yes
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
MLA-80
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
1
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Protein Concentration Method
BCA
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
0-500
EV concentration
Yes
|
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