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You searched for: EV200045 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200045 | 3/5 | Homo sapiens | PCI-13 |
(d)(U)C SEC (non-commercial) Filtration UF |
Ludwig Nils | 2019 | 50% | |
Study summaryFull title
All authors
Ludwig N, Hong CS, Ludwig S, Azambuja JH, Sharma P, Theodoraki MN, Whiteside TL.
Journal
Current Protocols in Immunology
Abstract
A method for isolation of exosomes from tumor cell supernatants or cancer patients' plasma is presen (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
SEC (non-commercial) Filtration UF Protein markers
EV: None
non-EV: Proteomics
no
Show all info
Study aim
Function/New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
PCI-13
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Other
Name other separation method
SEC (non-commercial)
PMID previous EV protein analysis
PMID 30042174 + PMID 30370252
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
PMID 30042174 / PMID 30370252
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Not Reported
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200045 | 5/5 | Homo sapiens | UMSCC47 |
(d)(U)C SEC (non-commercial) Filtration UF |
Ludwig Nils | 2019 | 38% | |
Study summaryFull title
All authors
Ludwig N, Hong CS, Ludwig S, Azambuja JH, Sharma P, Theodoraki MN, Whiteside TL.
Journal
Current Protocols in Immunology
Abstract
A method for isolation of exosomes from tumor cell supernatants or cancer patients' plasma is presen (show more...)
EV-METRIC
38% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
SEC (non-commercial) Filtration UF Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
UMSCC47
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Other
Name other separation method
SEC (non-commercial)
PMID previous EV protein analysis
30042174 + 30370252
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
PMID 30042174 / PMID 30370252
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV200045 | 1/5 | Homo sapiens | U-251 |
(d)(U)C SEC (non-commercial) Filtration UF |
Ludwig Nils | 2019 | 0% | |
Study summaryFull title
All authors
Ludwig N, Hong CS, Ludwig S, Azambuja JH, Sharma P, Theodoraki MN, Whiteside TL.
Journal
Current Protocols in Immunology
Abstract
A method for isolation of exosomes from tumor cell supernatants or cancer patients' plasma is presen (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
SEC (non-commercial) Filtration UF Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
U-251
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Other
Name other separation method
SEC (non-commercial)
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV200045 | 2/5 | Homo sapiens | Mel526 |
(d)(U)C SEC (non-commercial) Filtration UF |
Ludwig Nils | 2019 | 0% | |
Study summaryFull title
All authors
Ludwig N, Hong CS, Ludwig S, Azambuja JH, Sharma P, Theodoraki MN, Whiteside TL.
Journal
Current Protocols in Immunology
Abstract
A method for isolation of exosomes from tumor cell supernatants or cancer patients' plasma is presen (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
SEC (non-commercial) Filtration UF Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Mel526
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Other
Name other separation method
SEC (non-commercial)
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV200045 | 4/5 | Mus musculus | SVEC4-10 |
(d)(U)C SEC (non-commercial) Filtration UF |
Ludwig Nils | 2019 | 0% | |
Study summaryFull title
All authors
Ludwig N, Hong CS, Ludwig S, Azambuja JH, Sharma P, Theodoraki MN, Whiteside TL.
Journal
Current Protocols in Immunology
Abstract
A method for isolation of exosomes from tumor cell supernatants or cancer patients' plasma is presen (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
SEC (non-commercial) Filtration UF Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/New methodological development
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
SVEC4-10
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Other
Name other separation method
SEC (non-commercial)
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
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1 - 5 of 5 |
EV-TRACK ID | EV200045 | ||||
---|---|---|---|---|---|
species | Homo sapiens | Homo sapiens | Homo sapiens | Homo sapiens | Mus musculus |
sample type | Cell culture | Cell culture | Cell culture | Cell culture | Cell culture |
cell type | PCI-13 | UMSCC47 | U-251 | Mel526 | SVEC4-10 |
condition | Control condition | Control condition | Control condition | Control condition | Control condition |
separation protocol | (d)(U)C SEC (non-commercial) Filtration UF | (d)(U)C SEC (non-commercial) Filtration UF | (d)(U)C SEC (non-commercial) Filtration UF | (d)(U)C SEC (non-commercial) Filtration UF | (d)(U)C SEC (non-commercial) Filtration UF |
Exp. nr. | 3 | 5 | 1 | 2 | 4 |
EV-METRIC % | 50 | 38 | 0 | 0 | 0 |