Search > Results
You searched for: EV200014 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200014 | 1/4 | Homo sapiens | IMR90 ER:STOP |
(d)(U)C qEV |
Wallis, Ryan | 2021 | 56% | |
Study summaryFull title
All authors
Ryan Wallis, Natasa Josipovic, Hannah Mizen, Arturo Robles‐Tenorio, Eleanor J. Tyler, Argyris Papantonis, Cleo L. Bishop
Journal
J Extracell Vesicles
Abstract
A hallmark of senescence is the acquisition of an enhanced secretome comprising inflammatory mediato (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: TSG101/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
IMR90 ER:STOP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
6.20E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
T-865
Pelleting: speed (g)
99582
Commercial kit
qEV
Characterization: Protein analysis
PMID previous EV protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Not detected EV-associated proteins
TSG101/ CD9
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
PMID previous EV particle analysis
Extra particle analysis
NTA
Report type
Modus
Reported size (nm)
92
EV concentration
Yes
Particle yield
total concentration;Yes, other: 1.60E+10
|
||||||||
EV200014 | 2/4 | Homo sapiens | IMR90 ER:STOP | (d)(U)C | Wallis, Ryan | 2021 | 56% | |
Study summaryFull title
All authors
Ryan Wallis, Natasa Josipovic, Hannah Mizen, Arturo Robles‐Tenorio, Eleanor J. Tyler, Argyris Papantonis, Cleo L. Bishop
Journal
J Extracell Vesicles
Abstract
A hallmark of senescence is the acquisition of an enhanced secretome comprising inflammatory mediato (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ IL-8/ CD9
non-EV: Calnexin Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
IMR90 ER:STOP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
6.20E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
T-865
Pelleting: speed (g)
99582
Characterization: Protein analysis
PMID previous EV protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ TSG101
Detected contaminants
Calnexin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
IL-8
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
PMID previous EV particle analysis
Extra particle analysis
NTA
Report type
Modus
Reported size (nm)
85.2
EV concentration
Yes
Particle yield
total concentration;Yes, other: 2.20E+11
|
||||||||
EV200014 | 3/4 | Homo sapiens | IMR90 ER:RAS |
(d)(U)C qEV |
Wallis, Ryan | 2021 | 56% | |
Study summaryFull title
All authors
Ryan Wallis, Natasa Josipovic, Hannah Mizen, Arturo Robles‐Tenorio, Eleanor J. Tyler, Argyris Papantonis, Cleo L. Bishop
Journal
J Extracell Vesicles
Abstract
A hallmark of senescence is the acquisition of an enhanced secretome comprising inflammatory mediato (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
HRas:ER Oncogene Induced Senescence Model
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: TSG101/ IL-8/ CD9
non-EV: Calnexin Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
IMR90 ER:RAS
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
4.40E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
T-865
Pelleting: speed (g)
99582
Commercial kit
qEV
Characterization: Protein analysis
PMID previous EV protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ TSG101
Not detected contaminants
Calnexin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
IL-8
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
PMID previous EV particle analysis
Extra particle analysis
NTA
Report type
Modus
Reported size (nm)
107
EV concentration
Yes
Particle yield
total concentration;Yes, other: 8.80E+10
|
||||||||
EV200014 | 4/4 | Homo sapiens | IMR90 ER:RAS | (d)(U)C | Wallis, Ryan | 2021 | 56% | |
Study summaryFull title
All authors
Ryan Wallis, Natasa Josipovic, Hannah Mizen, Arturo Robles‐Tenorio, Eleanor J. Tyler, Argyris Papantonis, Cleo L. Bishop
Journal
J Extracell Vesicles
Abstract
A hallmark of senescence is the acquisition of an enhanced secretome comprising inflammatory mediato (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
HRas:ER Oncogene Induced Senescence Model
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ IL-8/ CD9
non-EV: Calnexin Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
IMR90 ER:RAS
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
4.40E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
T-865
Pelleting: speed (g)
99582
Characterization: Protein analysis
PMID previous EV protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ TSG101
Detected contaminants
Calnexin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
IL-8
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
PMID previous EV particle analysis
Extra particle analysis
NTA
Report type
Modus
Reported size (nm)
102
EV concentration
Yes
Particle yield
total concentration;Yes, other: 4.50E+11
|
||||||||
1 - 4 of 4 |
EV-TRACK ID | EV200014 | |||
---|---|---|---|---|
species | Homo sapiens | |||
sample type | Cell culture | |||
cell type | IMR90 ER:STOP | IMR90 ER:STOP | IMR90 ER:RAS | IMR90 ER:RAS |
condition | Control condition | Control condition | HRas:ER Oncogene Induced Senescence Model | HRas:ER Oncogene Induced Senescence Model |
separation protocol | (d)(U)C qEV | (d)(U)C | (d)(U)C qEV | (d)(U)C |
Exp. nr. | 1 | 2 | 3 | 4 |
EV-METRIC % | 56 | 56 | 56 | 56 |