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You searched for: EV190104 (EV-TRACK ID)
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Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190104 | 1/2 | Mus musculus | Cell culture supernatant | (d)(U)C | Swatler J | 2019 | 78% | |
Study summaryFull title
All authors
Swatler J, Dudka W, Bugajski L, Brewinska-Olchowik M, Kozlowska E, Piwocka K.
Journal
Eur J Immunol
Abstract
Mechanisms driving immunosuppression in chronic myeloid leukemia are mostly unknown. We show that le (show more...)
EV-METRIC
78% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Flotillin1/ Alix/ beta-actin/ TSG101/ HSP70/ CD81
non-EV: Grp78/ TOM20 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
32D
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Not specified
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
100
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
140000
Wash: volume per pellet (ml)
55
Wash: time (min)
100
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
140000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Detected EV-associated proteins
Flotillin1/ Alix/ beta-actin/ TSG101/ HSP70/ CD81
Not detected contaminants
Grp78/ TOM20
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
120
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
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EV190104 | 2/2 | Mus musculus | Cell culture supernatant | (d)(U)C | Swatler J | 2019 | 78% | |
Study summaryFull title
All authors
Swatler J, Dudka W, Bugajski L, Brewinska-Olchowik M, Kozlowska E, Piwocka K.
Journal
Eur J Immunol
Abstract
Mechanisms driving immunosuppression in chronic myeloid leukemia are mostly unknown. We show that le (show more...)
EV-METRIC
78% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
chronic myeloid leukemia (overexpresssion of BCR-ABL)
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Flotillin1/ Alix/ beta-actin/ TSG101/ HSP70/ CD81
non-EV: Grp78/ TOM20 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Sample Condition
chronic myeloid leukemia (overexpresssion of BCR-ABL)
EV-producing cells
32D
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Not specified
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
100
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
140000
Wash: volume per pellet (ml)
55
Wash: time (min)
100
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
140000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Detected EV-associated proteins
Flotillin1/ Alix/ beta-actin/ TSG101/ HSP70/ CD81
Not detected contaminants
Grp78/ TOM20
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
135.9
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
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