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You searched for: EV180070 (EV-TRACK ID)

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Experiment number
  • If needed, multiple experiments were identified in a single publication based on differing sample types, isolation protocols and/or vesicle types of interest.
Species
  • Species of origin of the EVs.
Isolation protocol
  • Gives a short, non-chronological overview of the different steps of the isolation protocol.
    • dUC = differential ultracentrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
Details EV-TRACK ID Experiment nr. Species Sample type Isolation protocol First author Year EV-METRIC
EV180070 2/5 Homo sapiens Cell culture supernatant (Differential) (ultra)centrifugation
Ultrafiltration
dUC
UF
Balducci E 2019 29%

Study summary

Full title
All authors
Balducci E, Leroyer AS, Lacroix R, Robert S, Todorova D, Simoncini S, Lyonnet L, Chareyre C, Zaegel-Faucher O, Micallef J, Poizot-Martin I, Roll P, Dignat-George F.
Journal
Sci Rep
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host resp (show more...)Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host responses that involves not only CD4 T cells, but also cells of the microenvironment, which are not directly infected, such as endothelial cells. The mechanisms triggering HIV-1-associated vascular alterations remain poorly understood. Extracellular vesicles (EVs), implicated in cell-to-cell communication, have been recently described as carriers of microRNAs (miRNAs). Here, we show that miR-146b-5p is upregulated in both CD4 T cells, CD4 T cell-derived EVs and circulating EVs obtained from antiretroviral therapy-naive HIV-1-infected patients. We further demonstrate that EVs from T cell line overexpressing miR-146b-5p mimics (miR-146b-EVs): 1) protect their miRNA cargo from RNase degradation, 2) transfer miR-146b-5p mimics into endothelial cells and 3) reduce endothelial inflammatory responses in vitro and in vivo in the lungs of mice through the downregulation of nuclear factor-κB-responsive molecules. These data advance our understanding on chronic inflammatory responses affecting endothelial homeostasis, in infectious and non-infectious diseases and pave the way for potential new anti-inflammatory strategies. (hide)
EV-METRIC
29% (60th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Isolation method: density gradient, at least as validation of results attributed to EVs
EV density
Isolation method: reporting of obtained EV density
ultracentrifugation specifics
Isolation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
T cells derived from HIV-1-infected patient
Focus vesicles
extracellular vesicle
Isolation protocol
Isolation protocol
  • Gives a short, non-chronological overview of the
    different steps of the isolation protocol.
    • dUC = differential ultracentrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
(Differential) (ultra)centrifugation + Ultrafiltration + dUC + UF
Protein markers
EV: CD45/ CD4/ TCRalfabeta/ / CD3
non-EV:
Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
T cells derived from HIV-1-infected patient
EV-producing cells
primary CD4 T cells
EV-harvesting Medium
Serum-containing medium
Isolation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
3-laser Navios flow cytometer (Beckman-Coulter)
Calibration bead size
0.1-0.9
Detected EV-associated proteins
CD45/ CD3/ CD4
Not detected EV-associated proteins
TCRalfabeta
Characterization: RNA analysis
RNAse treatment
Moment of RNAse treatment
RNAse type
RNAse concentration
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
347
Particle analysis: flow cytometry
Flow cytometer type
3-laser Navios flow cytometer (Beckman-Coulter)
Hardware adjustment
Calibration bead size
0.1
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
347 +/- 148
EV180070 3/5 Homo sapiens Cell culture supernatant dUC
(Differential) (ultra)centrifugation
Filtration
Balducci E 2019 14%

Study summary

Full title
All authors
Balducci E, Leroyer AS, Lacroix R, Robert S, Todorova D, Simoncini S, Lyonnet L, Chareyre C, Zaegel-Faucher O, Micallef J, Poizot-Martin I, Roll P, Dignat-George F.
Journal
Sci Rep
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host resp (show more...)Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host responses that involves not only CD4 T cells, but also cells of the microenvironment, which are not directly infected, such as endothelial cells. The mechanisms triggering HIV-1-associated vascular alterations remain poorly understood. Extracellular vesicles (EVs), implicated in cell-to-cell communication, have been recently described as carriers of microRNAs (miRNAs). Here, we show that miR-146b-5p is upregulated in both CD4 T cells, CD4 T cell-derived EVs and circulating EVs obtained from antiretroviral therapy-naive HIV-1-infected patients. We further demonstrate that EVs from T cell line overexpressing miR-146b-5p mimics (miR-146b-EVs): 1) protect their miRNA cargo from RNase degradation, 2) transfer miR-146b-5p mimics into endothelial cells and 3) reduce endothelial inflammatory responses in vitro and in vivo in the lungs of mice through the downregulation of nuclear factor-κB-responsive molecules. These data advance our understanding on chronic inflammatory responses affecting endothelial homeostasis, in infectious and non-infectious diseases and pave the way for potential new anti-inflammatory strategies. (hide)
EV-METRIC
14% (39th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Isolation method: density gradient, at least as validation of results attributed to EVs
EV density
Isolation method: reporting of obtained EV density
ultracentrifugation specifics
Isolation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Isolation protocol
Isolation protocol
  • Gives a short, non-chronological overview of the
    different steps of the isolation protocol.
    • dUC = differential ultracentrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
dUC + (Differential) (ultra)centrifugation + Filtration
Protein markers
EV: CD45/ CD4/ TCRalfabeta/ / CD3
non-EV:
Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
CCRF-CEM
EV-harvesting Medium
Serum-containing medium
Isolation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
3-laser Navios flow cytometer (Beckman-Coulter)
Calibration bead size
0.1-0.9
Detected EV-associated proteins
CD45/ CD4
Not detected EV-associated proteins
CD3/ TCRalfabeta
Characterization: RNA analysis
RNAse treatment
Moment of RNAse treatment
Before
RNAse type
RNase A
RNAse concentration
0.005
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
321+/-155
EM
EM-type
Report size (nm)
EV concentration
EV180070 1/5 Homo sapiens Cell culture supernatant (Differential) (ultra)centrifugation
Ultrafiltration
dUC
UF
Balducci E 2019 0%

Study summary

Full title
All authors
Balducci E, Leroyer AS, Lacroix R, Robert S, Todorova D, Simoncini S, Lyonnet L, Chareyre C, Zaegel-Faucher O, Micallef J, Poizot-Martin I, Roll P, Dignat-George F.
Journal
Sci Rep
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host resp (show more...)Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host responses that involves not only CD4 T cells, but also cells of the microenvironment, which are not directly infected, such as endothelial cells. The mechanisms triggering HIV-1-associated vascular alterations remain poorly understood. Extracellular vesicles (EVs), implicated in cell-to-cell communication, have been recently described as carriers of microRNAs (miRNAs). Here, we show that miR-146b-5p is upregulated in both CD4 T cells, CD4 T cell-derived EVs and circulating EVs obtained from antiretroviral therapy-naive HIV-1-infected patients. We further demonstrate that EVs from T cell line overexpressing miR-146b-5p mimics (miR-146b-EVs): 1) protect their miRNA cargo from RNase degradation, 2) transfer miR-146b-5p mimics into endothelial cells and 3) reduce endothelial inflammatory responses in vitro and in vivo in the lungs of mice through the downregulation of nuclear factor-κB-responsive molecules. These data advance our understanding on chronic inflammatory responses affecting endothelial homeostasis, in infectious and non-infectious diseases and pave the way for potential new anti-inflammatory strategies. (hide)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Isolation method: density gradient, at least as validation of results attributed to EVs
EV density
Isolation method: reporting of obtained EV density
ultracentrifugation specifics
Isolation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Isolation protocol
Isolation protocol
  • Gives a short, non-chronological overview of the
    different steps of the isolation protocol.
    • dUC = differential ultracentrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
(Differential) (ultra)centrifugation + Ultrafiltration + dUC + UF
Protein markers
EV:
non-EV:
Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
primary CD4 T cells
EV-harvesting Medium
Serum-containing medium
Isolation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Calibration bead size
Detected EV-associated proteins
Characterization: RNA analysis
RNAse treatment
Moment of RNAse treatment
RNAse type
RNAse concentration
Characterization: Particle analysis
EM
EM-type
Report size (nm)
EV concentration
EV180070 4/5 Homo sapiens Blood plasma dUC
Filtration
Balducci E 2019 0%

Study summary

Full title
All authors
Balducci E, Leroyer AS, Lacroix R, Robert S, Todorova D, Simoncini S, Lyonnet L, Chareyre C, Zaegel-Faucher O, Micallef J, Poizot-Martin I, Roll P, Dignat-George F.
Journal
Sci Rep
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host resp (show more...)Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host responses that involves not only CD4 T cells, but also cells of the microenvironment, which are not directly infected, such as endothelial cells. The mechanisms triggering HIV-1-associated vascular alterations remain poorly understood. Extracellular vesicles (EVs), implicated in cell-to-cell communication, have been recently described as carriers of microRNAs (miRNAs). Here, we show that miR-146b-5p is upregulated in both CD4 T cells, CD4 T cell-derived EVs and circulating EVs obtained from antiretroviral therapy-naive HIV-1-infected patients. We further demonstrate that EVs from T cell line overexpressing miR-146b-5p mimics (miR-146b-EVs): 1) protect their miRNA cargo from RNase degradation, 2) transfer miR-146b-5p mimics into endothelial cells and 3) reduce endothelial inflammatory responses in vitro and in vivo in the lungs of mice through the downregulation of nuclear factor-κB-responsive molecules. These data advance our understanding on chronic inflammatory responses affecting endothelial homeostasis, in infectious and non-infectious diseases and pave the way for potential new anti-inflammatory strategies. (hide)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Isolation method: density gradient, at least as validation of results attributed to EVs
EV density
Isolation method: reporting of obtained EV density
ultracentrifugation specifics
Isolation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Isolation protocol
Isolation protocol
  • Gives a short, non-chronological overview of the
    different steps of the isolation protocol.
    • dUC = differential ultracentrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
dUC + Filtration
Protein markers
EV:
non-EV:
Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
Control condition
Isolation Method
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Calibration bead size
Detected EV-associated proteins
Characterization: RNA analysis
RNAse treatment
Moment of RNAse treatment
RNAse type
RNAse concentration
Characterization: Particle analysis
EM
EM-type
Report size (nm)
EV concentration
EV180070 5/5 Homo sapiens Blood plasma dUC
Filtration
Balducci E 2019 0%

Study summary

Full title
All authors
Balducci E, Leroyer AS, Lacroix R, Robert S, Todorova D, Simoncini S, Lyonnet L, Chareyre C, Zaegel-Faucher O, Micallef J, Poizot-Martin I, Roll P, Dignat-George F.
Journal
Sci Rep
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host resp (show more...)Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host responses that involves not only CD4 T cells, but also cells of the microenvironment, which are not directly infected, such as endothelial cells. The mechanisms triggering HIV-1-associated vascular alterations remain poorly understood. Extracellular vesicles (EVs), implicated in cell-to-cell communication, have been recently described as carriers of microRNAs (miRNAs). Here, we show that miR-146b-5p is upregulated in both CD4 T cells, CD4 T cell-derived EVs and circulating EVs obtained from antiretroviral therapy-naive HIV-1-infected patients. We further demonstrate that EVs from T cell line overexpressing miR-146b-5p mimics (miR-146b-EVs): 1) protect their miRNA cargo from RNase degradation, 2) transfer miR-146b-5p mimics into endothelial cells and 3) reduce endothelial inflammatory responses in vitro and in vivo in the lungs of mice through the downregulation of nuclear factor-κB-responsive molecules. These data advance our understanding on chronic inflammatory responses affecting endothelial homeostasis, in infectious and non-infectious diseases and pave the way for potential new anti-inflammatory strategies. (hide)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Isolation method: density gradient, at least as validation of results attributed to EVs
EV density
Isolation method: reporting of obtained EV density
ultracentrifugation specifics
Isolation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Blood plasma
Sample origin
HIV-1-infected
Focus vesicles
extracellular vesicle
Isolation protocol
Isolation protocol
  • Gives a short, non-chronological overview of the
    different steps of the isolation protocol.
    • dUC = differential ultracentrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
dUC + Filtration
Protein markers
EV:
non-EV:
Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Sample Condition
HIV-1-infected
Isolation Method
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Calibration bead size
Detected EV-associated proteins
Characterization: RNA analysis
RNAse treatment
Moment of RNAse treatment
RNAse type
RNAse concentration
Characterization: Particle analysis
EM
EM-type
Report size (nm)
EV concentration
1 - 5 of 5
  • dUC = differential ultracentrifugation
  • DG = density gradient
  • UF = ultrafiltration
  • SEC = size-exclusion chromatography
EV-TRACK ID
EV180070
species
Homo sapiens
sample type
Cell culture
Cell culture
Cell culture
Blood plasma
Blood plasma
cell type
primary CD4 T cells
CCRF-CEM
primary CD4 T cells
NA
NA
medium
Serum-containing
medium
Serum-containing
medium
Serum-containing
medium
NA
NA
condition
T cells
derived from
HIV-1-infected patient
Control condition
Control condition
Control condition
HIV-1-infected
isolation protocol
dUC
Ultrafiltration
dUC
UF
dUC
dUC
Filtration
dUC
Ultrafiltration
dUC
UF
dUC
Filtration
dUC
Filtration
Exp. nr.
2
3
1
4
5
EV-METRIC %
29
14
0
0
0