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You searched for: EV180070 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV180070 | 2/5 | Homo sapiens | primary CD4 T cells |
(d)(U)C UF |
Balducci E | 2019 | 29% | |
Study summaryFull title
All authors
Balducci E, Leroyer AS, Lacroix R, Robert S, Todorova D, Simoncini S, Lyonnet L, Chareyre C, Zaegel-Faucher O, Micallef J, Poizot-Martin I, Roll P, Dignat-George F.
Journal
Sci Rep
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host resp (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
T cells derived from HIV-1-infected patient
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: CD45/ CD4/ TCRalfabeta/ / CD3
non-EV: Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary CD4 T cells
EV-harvesting Medium
Serum-containing medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
3-laser Navios flow cytometer (Beckman-Coulter)
Calibration bead size
0.1-0.9
Antibody details provided?
No
Detected EV-associated proteins
CD45/ CD3/ CD4
Not detected EV-associated proteins
TCRalfabeta
Characterization: RNA analysis
RNA analysis
Type
Other;(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Moment of RNAse treatment
RNAse type
RNAse concentration
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
347
Particle analysis: flow cytometry
Flow cytometer type
3-laser Navios flow cytometer (Beckman-Coulter)
Hardware adjustment
Calibration bead size
0.1
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
347 +/- 148
|
||||||||
EV180070 | 3/5 | Homo sapiens | CCRF-CEM | (d)(U)C | Balducci E | 2019 | 14% | |
Study summaryFull title
All authors
Balducci E, Leroyer AS, Lacroix R, Robert S, Todorova D, Simoncini S, Lyonnet L, Chareyre C, Zaegel-Faucher O, Micallef J, Poizot-Martin I, Roll P, Dignat-George F.
Journal
Sci Rep
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host resp (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD45/ CD4/ TCRalfabeta/ / CD3
non-EV: Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
CCRF-CEM
EV-harvesting Medium
Serum-containing medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
3-laser Navios flow cytometer (Beckman-Coulter)
Calibration bead size
0.1-0.9
Antibody details provided?
No
Detected EV-associated proteins
CD45/ CD4
Not detected EV-associated proteins
CD3/ TCRalfabeta
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Other
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
Before
RNAse type
RNase A
RNAse concentration
0.005
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
321+/-155
EM
EM-type
Report size (nm)
EV concentration
|
||||||||
EV180070 | 1/5 | Homo sapiens | primary CD4 T cells |
(d)(U)C UF |
Balducci E | 2019 | 0% | |
Study summaryFull title
All authors
Balducci E, Leroyer AS, Lacroix R, Robert S, Todorova D, Simoncini S, Lyonnet L, Chareyre C, Zaegel-Faucher O, Micallef J, Poizot-Martin I, Roll P, Dignat-George F.
Journal
Sci Rep
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host resp (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary CD4 T cells
EV-harvesting Medium
Serum-containing medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Calibration bead size
Antibody details provided?
No
Detected EV-associated proteins
Characterization: RNA analysis
RNA analysis
Type
Other;(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Moment of RNAse treatment
RNAse type
RNAse concentration
Characterization: Lipid analysis
No
EM
EM-type
Report size (nm)
EV concentration
|
||||||||
EV180070 | 4/5 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Balducci E | 2019 | 0% | |
Study summaryFull title
All authors
Balducci E, Leroyer AS, Lacroix R, Robert S, Todorova D, Simoncini S, Lyonnet L, Chareyre C, Zaegel-Faucher O, Micallef J, Poizot-Martin I, Roll P, Dignat-George F.
Journal
Sci Rep
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host resp (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Calibration bead size
Antibody details provided?
No
Detected EV-associated proteins
Characterization: RNA analysis
RNA analysis
Type
Other;(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Moment of RNAse treatment
RNAse type
RNAse concentration
Characterization: Lipid analysis
No
EM
EM-type
Report size (nm)
EV concentration
|
||||||||
EV180070 | 5/5 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Balducci E | 2019 | 0% | |
Study summaryFull title
All authors
Balducci E, Leroyer AS, Lacroix R, Robert S, Todorova D, Simoncini S, Lyonnet L, Chareyre C, Zaegel-Faucher O, Micallef J, Poizot-Martin I, Roll P, Dignat-George F.
Journal
Sci Rep
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection promotes a generalized activation of host resp (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
HIV-1-infected
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Calibration bead size
Antibody details provided?
No
Detected EV-associated proteins
Characterization: RNA analysis
RNA analysis
Type
Other;(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Moment of RNAse treatment
RNAse type
RNAse concentration
Characterization: Lipid analysis
No
EM
EM-type
Report size (nm)
EV concentration
|
||||||||
1 - 5 of 5 |
EV-TRACK ID | EV180070 | ||||
---|---|---|---|---|---|
species | Homo sapiens | ||||
sample type | Cell culture | Cell culture | Cell culture | Blood plasma | Blood plasma |
cell type | primary CD4 T cells | CCRF-CEM | primary CD4 T cells | NA | NA |
medium | Serum-containing medium | Serum-containing medium | Serum-containing medium | NA | NA |
condition | T cells derived from HIV-1-infected patient | Control condition | Control condition | Control condition | HIV-1-infected |
separation protocol | (d)(U)C UF | (d)(U)C | (d)(U)C UF | (d)(U)C Filtration | (d)(U)C Filtration |
Exp. nr. | 2 | 3 | 1 | 4 | 5 |
EV-METRIC % | 29 | 14 | 0 | 0 | 0 |