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You searched for: EV170015 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV170015 | 1/2 | Homo sapiens | NPCE cells |
50% PEG-8000, 0.5M NaCl, mixed with the conditioned medium 1:5 v/v respectively and incubated overnight at 4°C. The mixtures were centrifuged at 1500g for 30 minutes to pellet the EVs. (d)(U)C Filtration |
Tabak, Saray | 2018 | 0% | |
Study summaryFull title
All authors
Tabak S, Schreiber-Avissar S, Beit-Yannai E.
Journal
J Cell Mol Med
Abstract
The role of extracellular vesicles (EVs) as signal mediators has been described in many biological f (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
50% PEG-8000, 0.5M NaCl, mixed with the conditioned medium 1:5 v/v respectively and incubated overnight at 4°C. The mixtures were centrifuged at 1500g for 30 minutes to pellet the EVs.
(d)(U)C Filtration Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
NPCE cells
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Other
Name other separation method
50% PEG-8000, 0.5M NaCl, mixed with the conditioned medium 1:5 v/v respectively and incubated overnight at 4°C. The mixtures were centrifuged at 1500g for 30 minutes to pellet the EVs.
Characterization: Protein analysis
None
Protein Concentration Method
Bradford
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Mean
Reported size (nm)
98±10
EV concentration
Yes
Extra information
The precipated pellet containing the EVs was re-suspended in PBS and pelleted by ultra-centrifugation of 100,000g for 70 minutes at 4°C. The final EVs pelleted were suspended in 1mL PBS and were stored at -80˚C till use.
|
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EV170015 | 2/2 | Homo sapiens | RPE cells |
50% PEG-8000, 0.5M NaCl, mixed with the conditioned medium 1:5 v/v respectively and incubated overnight at 4°C. The mixtures were centrifuged at 1500g for 30 minutes to pellet the EVs. (d)(U)C Filtration |
Tabak, Saray | 2018 | 0% | |
Study summaryFull title
All authors
Tabak S, Schreiber-Avissar S, Beit-Yannai E.
Journal
J Cell Mol Med
Abstract
The role of extracellular vesicles (EVs) as signal mediators has been described in many biological f (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
50% PEG-8000, 0.5M NaCl, mixed with the conditioned medium 1:5 v/v respectively and incubated overnight at 4°C. The mixtures were centrifuged at 1500g for 30 minutes to pellet the EVs.
(d)(U)C Filtration Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
RPE cells
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Other
Name other separation method
50% PEG-8000, 0.5M NaCl, mixed with the conditioned medium 1:5 v/v respectively and incubated overnight at 4°C. The mixtures were centrifuged at 1500g for 30 minutes to pellet the EVs.
Characterization: Protein analysis
None
Protein Concentration Method
Bradford
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Mean
Reported size (nm)
128±11
EV concentration
Yes
Extra information
The precipated pellet containing the EVs was re-suspended in PBS and pelleted by ultra-centrifugation of 100,000g for 70 minutes at 4°C. The final EVs pelleted were suspended in 1mL PBS and were stored at -80˚C till use.
|
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1 - 2 of 2 |
EV-TRACK ID | EV170015 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | NPCE cells | RPE cells |
condition | Control condition | Control condition |