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You searched for: EV150106 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150106 | 1/1 | Homo sapiens | Cell culture supernatant | DG (d)(U)C |
van Balkom BW | 2015 | 56% | |
Study summaryFull title
All authors
van Balkom BW, Eisele AS, Pegtel DM, Bervoets S, Verhaar MC.
Journal
J Extracell Vesicles
Abstract
Exosomes are small vesicles that mediate cell-cell communication. They contain proteins, lipids and (show more...)
EV-METRIC
56% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
HMEC-1
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: GAPDH/ Flotillin1/ CD9/
non-EV: Histone H2A.X/ Lamin A / C/ ATP5A/ Tom20 Proteomics
no
EV density (g/ml)
1.07 - 1.12
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Control condition
EV-producing cells
HMEC-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
1h at 200000g
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Wash: time (min)
60
Wash: Rotor Type
Not specified
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Density medium
Sucrose
Type
Continuous
Lowest density fraction
0.25M
Highest density fraction
2.0M
Sample volume (mL)
0.25
Orientation
Bottom-up
Rotor type
Not specified
Speed (g)
190000
Duration (min)
960
Fraction volume (mL)
0.4
Fraction processing
None
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
GAPDH/ Flotillin1/ CD9
Not detected contaminants
Lamin A/C/ Histone H2A.X/ ATP5A/ Tom20
EM
EM-type
Transmission-EM
Image type
Wide-field
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