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You searched for: EV150103 (EV-TRACK ID)
Showing 1 - 7 of 7
Showing 1 - 7 of 7
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150103 | 7/7 | Homo sapiens | Cell culture supernatant | dUC | Dieudé M | 2015 | 55% | |
Study summaryFull title
All authors
Dieudé M, Bell C, Turgeon J, Beillevaire D, Pomerleau L, Yang B, Hamelin K, Qi S, Pallet N, Béland C, Dhahri W, Cailhier JF, Rousseau M, Duchez AC, Lévesque T, Lau A, Rondeau C, Gingras D, Muruve D, Rivard A, Cardinal H, Perreault C, Desjardins M, Boilard É, Thibault P, Hébert MJ
Journal
J Transl Med
Abstract
Autoantibodies to components of apoptotic cells, such as anti-perlecan antibodies, contribute to rej (show more...)
EV-METRIC
55% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Apoptosis
Focus vesicles
apoptotic exosome-like vesicle
Separation protocol
Separation protocol
dUC
Adj. k-factor
127.9 (pelleting)
Protein markers
EV: LG3/ Fibronectin/ proteasome-alpha3/ Syntenin/ TCTP
non-EV: Tubulin/ GM130 Proteomics
yes
Show all info
Study aim
Function, Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Apoptosis
EV-producing cells
HUVEC
EV-harvesting Medium
Serum free medium
Cell viability
75
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Between 50,000 g and 100,000 g Pelleting: time(min)
1080
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
200000
Pelleting: adjusted k-factor
127.9
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Concentration
300
Western Blot
Detected EV-associated proteins
Syntenin, Fibronectin, TCTP, proteasome-alpha3, LG3
Not detected contaminants
GM130, Tubulin
Proteomics database
Yes
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
BDCantoII Special Order Research Product
Hardware adjustment
This high sensitivity Flow cytometer (hsFCM) is equipped with a small particle option. The forward scatter (FSC) on this dedicated equipment is coupled to a photomultiplier tube (PMT) with a 488 nm solid state;100mW output blue laser (rather than the conventional 20 mW);and includes a 633nmHeNe;20mW output red laser and a 405 nm solid state diode;50mW output violet laser. The hsFCM includes a FSC-PMT and a Fourier optical transformation unit;which reduces the background noise and increases the angle of diffusion;therby enhancing the detection of small-diameter particles.
Calibration bead size
0.09,0.45,0.84,1,3.2
Report type
Median
Reported size (nm)
100-200
EV concentration
Yes
Particle yield
3.50E+07 particles/million cells
EM
EM-type
Transmission-EM/ Immune-EM
Proteïns
LG3;proteasome-alpha3
Image type
Close-up, Wide-field
|
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EV150103 | 5/7 | Homo sapiens | Cell culture supernatant | dUC | Dieudé M | 2015 | 44% | |
Study summaryFull title
All authors
Dieudé M, Bell C, Turgeon J, Beillevaire D, Pomerleau L, Yang B, Hamelin K, Qi S, Pallet N, Béland C, Dhahri W, Cailhier JF, Rousseau M, Duchez AC, Lévesque T, Lau A, Rondeau C, Gingras D, Muruve D, Rivard A, Cardinal H, Perreault C, Desjardins M, Boilard É, Thibault P, Hébert MJ
Journal
J Transl Med
Abstract
Autoantibodies to components of apoptotic cells, such as anti-perlecan antibodies, contribute to rej (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Apoptosis
Focus vesicles
apoptotic body
Separation protocol
Separation protocol
dUC
Adj. k-factor
127.9 (pelleting)
Protein markers
EV: Tubulin/ TCTP/ Fibronectin/ Syntenin/ LG3/ GM130
non-EV: None Proteomics
yes
Show all info
Study aim
Function, Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Sample Condition
Apoptosis
EV-producing cells
HUVEC
EV-harvesting Medium
Serum free medium
Cell viability
75
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 50,000 g and 100,000 g Pelleting: time(min)
1080
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
200000
Pelleting: adjusted k-factor
127.9
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Concentration
2000
Western Blot
Detected EV-associated proteins
Syntenin, Fibronectin, TCTP, GM130, Tubulin, LG3
Proteomics database
Yes
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
BDCantoII Special Order Research Product
Hardware adjustment
This high sensitivity Flow cytometer (hsFCM) is equipped with a small particle option. The forward scatter (FSC) on this dedicated equipment is coupled to a photomultiplier tube (PMT) with a 488 nm solid state;100mW output blue laser (rather than the conventional 20 mW);and includes a 633nmHeNe;20mW output red laser and a 405 nm solid state diode;50mW output violet laser. The hsFCM includes a FSC-PMT and a Fourier optical transformation unit;which reduces the background noise and increases the angle of diffusion;therby enhancing the detection of small-diameter particles.
Calibration bead size
0.09,0.45,0.84,1,3.2
Report type
Median
Reported size (nm)
100-200
EV concentration
Yes
Particle yield
3.50E+07 particles/million cells
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV150103 | 4/7 | Mus musculus | Serum | dUC | Dieudé M | 2015 | 22% | |
Study summaryFull title
All authors
Dieudé M, Bell C, Turgeon J, Beillevaire D, Pomerleau L, Yang B, Hamelin K, Qi S, Pallet N, Béland C, Dhahri W, Cailhier JF, Rousseau M, Duchez AC, Lévesque T, Lau A, Rondeau C, Gingras D, Muruve D, Rivard A, Cardinal H, Perreault C, Desjardins M, Boilard É, Thibault P, Hébert MJ
Journal
J Transl Med
Abstract
Autoantibodies to components of apoptotic cells, such as anti-perlecan antibodies, contribute to rej (show more...)
EV-METRIC
22% (73rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Acute kidney injury model
Focus vesicles
exosome-like vesicle, membrane vesicle, nanovesicle
Separation protocol
Separation protocol
dUC
Adj. k-factor
127.9 (pelleting)
Protein markers
EV: LG3/ proteasome-alpha3
non-EV: None Proteomics
no
Show all info
Study aim
Function, Biomarker, Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Serum
Sample Condition
Acute kidney injury model
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Equal to or above 150,000 g
Between 50,000 g and 100,000 g Pelleting: time(min)
1080
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
200000
Pelleting: adjusted k-factor
127.9
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
LG3
Characterization: Particle analysis
EM
EM-type
Transmission-EM/ Immune-EM
Proteïns
proteasome-alpha3
Image type
Close-up, Wide-field
|
||||||||
EV150103 | 1/7 | Mus musculus | Cell culture supernatant | dUC | Dieudé M | 2015 | 11% | |
Study summaryFull title
All authors
Dieudé M, Bell C, Turgeon J, Beillevaire D, Pomerleau L, Yang B, Hamelin K, Qi S, Pallet N, Béland C, Dhahri W, Cailhier JF, Rousseau M, Duchez AC, Lévesque T, Lau A, Rondeau C, Gingras D, Muruve D, Rivard A, Cardinal H, Perreault C, Desjardins M, Boilard É, Thibault P, Hébert MJ
Journal
J Transl Med
Abstract
Autoantibodies to components of apoptotic cells, such as anti-perlecan antibodies, contribute to rej (show more...)
EV-METRIC
11% (26th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Apoptosis
Focus vesicles
apoptotic exosome-like vesicle
Separation protocol
Separation protocol
dUC
Adj. k-factor
127.9 (pelleting)
Protein markers
EV: LG3
non-EV: None Proteomics
no
Show all info
Study aim
Function, Biomarker, Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Sample Condition
Apoptosis
EV-producing cells
primary aorta-derived endothelial cells
EV-harvesting Medium
Serum free medium
Cell viability
80
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Between 50,000 g and 100,000 g Pelleting: time(min)
1080
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
200000
Pelleting: adjusted k-factor
127.9
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
LG3
Characterization: Particle analysis
EM
|
||||||||
EV150103 | 2/7 | Mus musculus | Cell culture supernatant | dUC | Dieudé M | 2015 | 11% | |
Study summaryFull title
All authors
Dieudé M, Bell C, Turgeon J, Beillevaire D, Pomerleau L, Yang B, Hamelin K, Qi S, Pallet N, Béland C, Dhahri W, Cailhier JF, Rousseau M, Duchez AC, Lévesque T, Lau A, Rondeau C, Gingras D, Muruve D, Rivard A, Cardinal H, Perreault C, Desjardins M, Boilard É, Thibault P, Hébert MJ
Journal
J Transl Med
Abstract
Autoantibodies to components of apoptotic cells, such as anti-perlecan antibodies, contribute to rej (show more...)
EV-METRIC
11% (26th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Apoptosis
Focus vesicles
apoptotic body
Separation protocol
Separation protocol
dUC
Adj. k-factor
127.9 (pelleting)
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function, Biomarker, Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Sample Condition
Apoptosis
EV-producing cells
primary aorta-derived endothelial cells
EV-harvesting Medium
Serum free medium
Cell viability
80
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 50,000 g and 100,000 g Pelleting: time(min)
1080
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
200000
Pelleting: adjusted k-factor
127.9
Characterization: Protein analysis
Protein Concentration Method
Not determined
Characterization: Particle analysis
EM
|
||||||||
EV150103 | 3/7 | Mus musculus | Serum | dUC | Dieudé M | 2015 | 11% | |
Study summaryFull title
All authors
Dieudé M, Bell C, Turgeon J, Beillevaire D, Pomerleau L, Yang B, Hamelin K, Qi S, Pallet N, Béland C, Dhahri W, Cailhier JF, Rousseau M, Duchez AC, Lévesque T, Lau A, Rondeau C, Gingras D, Muruve D, Rivard A, Cardinal H, Perreault C, Desjardins M, Boilard É, Thibault P, Hébert MJ
Journal
J Transl Med
Abstract
Autoantibodies to components of apoptotic cells, such as anti-perlecan antibodies, contribute to rej (show more...)
EV-METRIC
11% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
exosome-like vesicle, membrane vesicle, nanovesicle
Separation protocol
Separation protocol
dUC
Adj. k-factor
127.9 (pelleting)
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function, Biomarker, Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Serum
Sample Condition
Control condition
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Equal to or above 150,000 g
Between 50,000 g and 100,000 g Pelleting: time(min)
1080
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
200000
Pelleting: adjusted k-factor
127.9
Characterization: Protein analysis
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV150103 | 6/7 | Mus musculus | Serum | dUC | Dieudé M | 2015 | 11% | |
Study summaryFull title
All authors
Dieudé M, Bell C, Turgeon J, Beillevaire D, Pomerleau L, Yang B, Hamelin K, Qi S, Pallet N, Béland C, Dhahri W, Cailhier JF, Rousseau M, Duchez AC, Lévesque T, Lau A, Rondeau C, Gingras D, Muruve D, Rivard A, Cardinal H, Perreault C, Desjardins M, Boilard É, Thibault P, Hébert MJ
Journal
J Transl Med
Abstract
Autoantibodies to components of apoptotic cells, such as anti-perlecan antibodies, contribute to rej (show more...)
EV-METRIC
11% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Ischemic hindlimb model
Focus vesicles
exosome-like vesicle, nanovesicle
Separation protocol
Separation protocol
dUC
Adj. k-factor
127.9 (pelleting)
Protein markers
EV: LG3
non-EV: None Proteomics
no
Show all info
Study aim
Function, Biomarker, Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Serum
Sample Condition
Ischemic hindlimb model
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Equal to or above 150,000 g
Between 50,000 g and 100,000 g Pelleting: time(min)
1080
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
200000
Pelleting: adjusted k-factor
127.9
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
LG3
|
||||||||
1 - 7 of 7 |
EV-TRACK ID | EV150103 | ||||||
---|---|---|---|---|---|---|---|
species | Homo sapiens | Homo sapiens | Mus musculus | Mus musculus | Mus musculus | Mus musculus | Mus musculus |
sample type | Cell culture | Cell culture | Serum | Cell culture | Cell culture | Serum | Serum |
cell type | HUVEC | HUVEC | NA | primary aorta-derived endothelial cells | primary aorta-derived endothelial cells | NA | NA |
medium | Serum free medium | Serum free medium | NA | Serum free medium | Serum free medium | NA | NA |
condition | Apoptosis | Apoptosis | Acute kidney injury model | Apoptosis | Apoptosis | Control condition | Ischemic hindlimb model |
separation protocol | dUC | dUC | dUC | dUC | dUC | dUC | dUC |
EV subtype | No | No | NA | No | No | NA | NA |
vesicle related term | apoptotic exosome-like vesicle | apoptotic body | exosome-like vesicle membrane vesicle nanovesicle | apoptotic exosome-like vesicle | apoptotic body | exosome-like vesicle membrane vesicle nanovesicle | exosome-like vesicle nanovesicle |
Exp. nr. | 7 | 5 | 4 | 1 | 2 | 3 | 6 |
EV-METRIC % | 55 | 44 | 22 | 11 | 11 | 11 | 11 |