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You searched for: EV150060 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150060 | 3/4 | Homo sapiens | Semen |
(d)(U)C DG Filtration |
Madison MN | 2015 | 29% | |
Study summaryFull title
All authors
Madison MN, Jones PH, Okeoma CM
Journal
Virology
Abstract
Exosomes are membranous extracellular nanovesicles secreted by diverse cell types. Exosomes from hea (show more...)
EV-METRIC
29% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
255.8 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
255.8
Density gradient
Lowest density fraction
2.5
Highest density fraction
25
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
|
||||||||
EV150060 | 4/4 | Homo sapiens | Serum |
(d)(U)C DG Filtration |
Madison MN | 2015 | 29% | |
Study summaryFull title
All authors
Madison MN, Jones PH, Okeoma CM
Journal
Virology
Abstract
Exosomes are membranous extracellular nanovesicles secreted by diverse cell types. Exosomes from hea (show more...)
EV-METRIC
29% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
255.8 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
255.8
Density gradient
Lowest density fraction
2.5
Highest density fraction
25
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
|
||||||||
EV150060 | 1/4 | Homo sapiens | Semen |
(d)(U)C ExoQuick |
Madison MN | 2015 | 0% | |
Study summaryFull title
All authors
Madison MN, Jones PH, Okeoma CM
Journal
Virology
Abstract
Exosomes are membranous extracellular nanovesicles secreted by diverse cell types. Exosomes from hea (show more...)
EV-METRIC
0% (median: 25% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
|
||||||||
EV150060 | 2/4 | Homo sapiens | Serum |
(d)(U)C ExoQuick |
Madison MN | 2015 | 0% | |
Study summaryFull title
All authors
Madison MN, Jones PH, Okeoma CM
Journal
Virology
Abstract
Exosomes are membranous extracellular nanovesicles secreted by diverse cell types. Exosomes from hea (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
|
||||||||
1 - 4 of 4 |
EV-TRACK ID | EV150060 | |||
---|---|---|---|---|
species | Homo sapiens | |||
sample type | Semen | Serum | Semen | Serum |
condition | NAY | NAY | NAY | NAY |
separation protocol | (d)(U)C DG Filtration | (d)(U)C DG Filtration | (d)(U)C ExoQuick | (d)(U)C ExoQuick |
Exp. nr. | 3 | 4 | 1 | 2 |
EV-METRIC % | 29 | 29 | 0 | 0 |