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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV140406	1/1	Homo sapiens	Serum	silica tips to Mass Spectrometric	Ueda K	2014	17%
Study summary Full title Antibody-coupled monolithic silica microtips for highthroughput molecular profiling of circulating exosomes All authors Ueda K, Ishikawa N, Tatsuguchi A, Saichi N, Fujii R, Nakagawa H Journal Sci Rep Abstract Exosome-mediated signal transportation plays a variety of critical roles in cancer progression and m (show more...)Exosome-mediated signal transportation plays a variety of critical roles in cancer progression and metastasis. From the aspect of cancer diagnosis, circulating exosomes are ideal resources of biomarkers because molecular features of tumor cells are transcribed on them. However, isolating pure exosomes from body fluids is time-consuming and still major challenge to be addressed for comprehensive profiling of exosomal proteins and miRNAs. Here we constructed anti-CD9 antibody-coupled highly porous monolithic silica microtips which allowed automated rapid and reproducible exosome extraction from multiple clinical samples. We applied these tips to explore lung cancer biomarker proteins on exosomes by analyzing 46 serum samples. The mass spectrometric quantification of 1,369 exosomal proteins identified CD91 as a lung adenocarcinoma specific antigen on exosomes, which was further validated with CD9-CD91 exosome sandwich ELISA measuring 212 samples. Our simple device can promote not only biomarker discovery studies but also wide range of omics researches about exosomes. (hide) EV-METRIC 17% (61st percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Serum Sample origin NAY Focus vesicles exosomes Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture silica tips to Mass Spectrometric Protein markers EV: non-EV: Proteomics yes Show all info Study aim Technical Sample Species Homo sapiens Sample Type Serum Separation Method Other Name other separation method silica tips to Mass Spectrometric Characterization: Protein analysis

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EV-TRACK ID	EV140406
species	Homo sapiens
sample type	Serum
condition	NAY
separation protocol	silica tips to Mass Spectrometric
Exp. nr.	1
EV-METRIC %	17