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You searched for: EV140307 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140307 | 1/1 | Heligmosomoides polygyrus | Nematode culture |
(d)(U)C Filtration |
Buck AH | 2014 | 29% | |
Study summaryFull title
All authors
Buck AH, Coakley G, Simbari F, McSorley HJ, Quintana JF, Le Bihan T, Kumar S, Abreu-Goodger C, Lear M, Harcus Y, Ceroni A, Babayan SA, Blaxter M, Ivens A, Maizels RM
Journal
Nat Commun
Abstract
In mammalian systems RNA can move between cells via vesicles. Here we demonstrate that the gastroint (show more...)
EV-METRIC
29% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Nematode culture
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
276.6 (pelleting)
Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Heligmosomoides polygyrus
Sample Type
Nematode culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW40
Pelleting: adjusted k-factor
276.6
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
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1 - 1 of 1 |
EV-TRACK ID | EV140307 |
---|---|
species | Heligmosomoides polygyrus |
sample type | Nematode culture |
condition | NAY |
separation protocol | (d)(U)C Filtration |
Exp. nr. | 1 |
EV-METRIC % | 29 |