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You searched for: EV140203 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140203 | 1/1 | Mus musculus | NAY |
(d)(U)C DG Filtration |
Padro CJ | 2014 | 44% | |
Study summaryFull title
All authors
Padro CJ, Shawler TM, Gormley MG, Sanders VM
Journal
J Immunol
Abstract
Soluble CD23 plays a role in the positive regulation of an IgE response. Engagement of the ?2 adrene (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
255.8 (pelleting)
Protein markers
EV: ADAM10/ CD63
non-EV: Proteomics
no
EV density (g/ml)
1.05-1.1
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
255.8
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
1.00g/L
Highest density fraction
1.25g/L
Orientation
Top-down
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ ADAM10
ELISA
Antibody details provided?
No
Detected EV-associated proteins
ADAM10
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
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1 - 1 of 1 |
EV-TRACK ID | EV140203 |
---|---|
species | Mus musculus |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C DG Filtration |
Exp. nr. | 1 |
EV-METRIC % | 44 |