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You searched for: EV140179 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140179 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG UF |
Klinker MW | 2014 | 44% | |
Study summaryFull title
All authors
Klinker MW, Lizzio V, Reed TJ, Fox DA, Lundy SK
Journal
Front Immunol
Abstract
Immune suppression mediated by exosomes is an emerging concept with potentially immense utility for (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG UF Protein markers
EV: Beta-actin/ MHC2/ FasL
non-EV: FasL Proteomics
no
EV density (g/ml)
1.160
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60-240
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
1.03g/l
Highest density fraction
1.27g/l
Orientation
Top-down
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
MHC2/ FasL/ MHC2/ Beta-actin
Detected contaminants
FasL
ELISA
Antibody details provided?
No
Detected EV-associated proteins
MHC2/ FasL/ MHC2/ Beta-actin
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
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EV-TRACK ID | EV140179 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C DG UF |
Exp. nr. | 1 |
EV-METRIC % | 44 |