Search > Results
You searched for: EV140166 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140166 | 2/3 | Mus musculus | Pre-purified exosomes loaded with SPION5 | DG | Hood JL | 2014 | 67% | |
Study summaryFull title
All authors
Hood JL, Scott MJ, Wickline SA
Journal
Anal Biochem
Abstract
Development of exosome-based semisynthetic nanovesicles for diagnostic and therapeutic purposes requ (show more...)
EV-METRIC
67% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Pre-purified exosomes loaded with SPION5
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG
Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.16-1.19
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Pre-purified exosomes loaded with SPION5
Separation Method
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140166 | 1/3 | Mus musculus | Cell culture supernatant | dUC | Hood JL | 2014 | 14% | |
Study summaryFull title
All authors
Hood JL, Scott MJ, Wickline SA
Journal
Anal Biochem
Abstract
Development of exosome-based semisynthetic nanovesicles for diagnostic and therapeutic purposes requ (show more...)
EV-METRIC
14% (39th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
dUC
Adj. k-factor
564.7 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
120
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
564.7
Characterization: Particle analysis
DLS
|
||||||||
EV140166 | 3/3 | Mus musculus | Serum | dUC Filtration |
Hood JL | 2014 | 14% | |
Study summaryFull title
All authors
Hood JL, Scott MJ, Wickline SA
Journal
Anal Biochem
Abstract
Development of exosome-based semisynthetic nanovesicles for diagnostic and therapeutic purposes requ (show more...)
EV-METRIC
14% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
dUC + Filtration
Adj. k-factor
564.7 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Serum
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting: time(min)
120
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
564.7
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
DLS
|
||||||||
1 - 3 of 3 |
EV-TRACK ID | EV140166 | ||
---|---|---|---|
species | Mus musculus | ||
sample type | Pre-purified exosomes loaded with SPION5 | Cell culture | Serum |
medium | EV Depleted | ||
separation protocol | DG | dUC | dUC Filtration |
Exp. nr. | 2 | 1 | 3 |
EV-METRIC % | 67 | 14 | 14 |