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You searched for: EV140117 (EV-TRACK ID)
Showing 1 - 8 of 8
Showing 1 - 8 of 8
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140117 | 2/8 | Homo sapiens | Blood plasma | (d)(U)C | He M | 2014 | 44% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
44% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD81/ CD9
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140117 | 3/8 | Homo sapiens | Blood plasma | IAF Microfluidics |
He M | 2014 | 33% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
33% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF + Microfluidics
Protein markers
EV:
non-EV: Proteomics
no
TEM measurements
50-100
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140117 | 4/8 | Homo sapiens | Blood plasma | IAF Microfluidics |
He M | 2014 | 33% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
33% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF + Microfluidics
Protein markers
EV:
non-EV: Proteomics
no
TEM measurements
25-100
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140117 | 8/8 | Homo sapiens | Blood plasma | IAF Microfluidics |
He M | 2014 | 33% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
33% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF + Microfluidics
Protein markers
EV:
non-EV: Proteomics
no
TEM measurements
50-100
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140117 | 1/8 | Homo sapiens | Cell culture supernatant | (d)(U)C | He M | 2014 | 22% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
22% (45th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD81/ CD9
Characterization: Particle analysis
|
||||||||
EV140117 | 6/8 | Homo sapiens | Blood plasma | IAF Microfluidics |
He M | 2014 | 17% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
17% (42nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF + Microfluidics
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140117 | 7/8 | Homo sapiens | Blood plasma | IAF Microfluidics |
He M | 2014 | 17% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
17% (42nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF + Microfluidics
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140117 | 5/8 | Homo sapiens | Blood plasma | IAF Microfluidics |
He M | 2014 | 0% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
0% (median: 25% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF + Microfluidics
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
|
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