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You searched for: EV140046 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140046 | 1/1 | Bacillus subtilis | Bacteria | DG dUC Filtration UF |
Brown L | 2014 | 57% | |
Study summaryFull title
All authors
Brown L, Kessler A, Cabezas-Sanchez P, Luque-Garcia JL, Casadevall A
Journal
Mol Microbiol
Abstract
Previously, extracellular vesicle production in Gram-positive bacteria was dismissed due to the abse (show more...)
EV-METRIC
57% (95th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
DG + dUC + Filtration + UF
Protein markers
EV:
non-EV: Proteomics
yes
TEM measurements
137.69999999999999
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Bacillus subtilis
Sample Type
Bacteria
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting: time(min)
60
Density gradient
Only used for validation of main results
1
Density medium
Iodixanol
Lowest density fraction
10
Highest density fraction
30
Orientation
Bottom-up
Filtration steps
> 0.45 µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM/ immune EM
Proteïns
GXM
Image type
Close-up, Wide-field
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