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You searched for: EV140001 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140001 | 2/4 | Homo sapiens | Cell culture supernatant |
DG (d)(U)C Filtration |
Van Deun J | 2014 | 88% | |
Study summaryFull title
All authors
Van Deun J, Mestdagh P, Sormunen R, Cocquyt V, Vermaelen K, Vandesompele J, Bracke M, De Wever O, Hendrix A
Journal
J Extracell Vesicles
Abstract
Despite an enormous interest in the role of extracellular vesicles, including exosomes, in cancer an (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Protein markers
EV: Alix/ HSP70/ TSG101/ HSP90/ CD63
non-EV: Cell organelle protein/ Ago2 Proteomics
no
EV density (g/ml)
1.094
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Density gradient
Density medium
Iodixanol
Lowest density fraction
5
Highest density fraction
40
Orientation
Top-down
Rotor type
SW32.1
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ HSP90/ HSP70/ TSG101
Detected contaminants
Cell organelle protein/ Ago2
Characterization: Particle analysis
NTA
EM
EM-type
immune EM
Proteïns
CD63
Image type
Wide-field
|
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EV140001 | 1/4 | Homo sapiens | Cell culture supernatant |
(d)(U)C Filtration |
Van Deun J | 2014 | 67% | |
Study summaryFull title
All authors
Van Deun J, Mestdagh P, Sormunen R, Cocquyt V, Vermaelen K, Vandesompele J, Bracke M, De Wever O, Hendrix A
Journal
J Extracell Vesicles
Abstract
Despite an enormous interest in the role of extracellular vesicles, including exosomes, in cancer an (show more...)
EV-METRIC
67% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
138.6 (pelleting)
Protein markers
EV: Alix/ HSP70/ TSG101/ HSP90/ CD63
non-EV: Cell organelle protein/ Ago2 Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
180
Pelleting: rotor type
SW55
Pelleting: adjusted k-factor
138.6
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ HSP90/ HSP70/ TSG101
Detected contaminants
Cell organelle protein/ Ago2
Characterization: Particle analysis
NTA
EM
EM-type
immune EM
Proteïns
CD63
Image type
Wide-field
|
||||||||
EV140001 | 3/4 | Homo sapiens | Cell culture supernatant |
(d)(U)C Filtration Total Exosome Isolation |
Van Deun J | 2014 | 63% | |
Study summaryFull title
All authors
Van Deun J, Mestdagh P, Sormunen R, Cocquyt V, Vermaelen K, Vandesompele J, Bracke M, De Wever O, Hendrix A
Journal
J Extracell Vesicles
Abstract
Despite an enormous interest in the role of extracellular vesicles, including exosomes, in cancer an (show more...)
EV-METRIC
63% (89th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Total Exosome Isolation Adj. k-factor
0 (pelleting)
Protein markers
EV: Alix/ HSP70/ TSG101/ HSP90/ CD63
non-EV: Cell organelle protein/ Ago2 Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Obtain an EV pellet :
Yes
Pelleting: adjusted k-factor
NA
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ HSP90/ HSP70/ TSG101
Detected contaminants
Cell organelle protein/ Ago2
Characterization: Particle analysis
NTA
EM
EM-type
immune EM
Proteïns
CD63
Image type
Close-up
|
||||||||
EV140001 | 4/4 | Homo sapiens | Cell culture supernatant |
(d)(U)C ExoQuick Filtration |
Van Deun J | 2014 | 63% | |
Study summaryFull title
All authors
Van Deun J, Mestdagh P, Sormunen R, Cocquyt V, Vermaelen K, Vandesompele J, Bracke M, De Wever O, Hendrix A
Journal
J Extracell Vesicles
Abstract
Despite an enormous interest in the role of extracellular vesicles, including exosomes, in cancer an (show more...)
EV-METRIC
63% (89th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Filtration Protein markers
EV: Alix/ HSP70/ TSG101/ HSP90/ CD63
non-EV: Cell organelle protein/ Ago2 Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ HSP90/ HSP70/ TSG101
Detected contaminants
Cell organelle protein/ Ago2
Characterization: Particle analysis
NTA
EM
EM-type
immune EM
Proteïns
CD63
Image type
Close-up
|
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