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You searched for: EV130137 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130137 | 3/3 | Mus musculus Rattus norvegicus/rattus |
Cell culture supernatant | DG (d)(U)C Filtration |
Royo F | 2013 | 44% | |
Study summaryFull title
All authors
Royo F, Schlangen K, Palomo L, Gonzalez E, Conde-Vancells J, Berisa A, Aransay AM, Falcon-Perez JM
Journal
PLoS One
Abstract
The discovery that the cells communicate through emission of vesicles has opened new opportunities f (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Protein markers
EV: CD81/ TSG101/ Flotilin1/ Aip1
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.2;1.19-1.23
Show all info
Study aim
Omics
Sample
Species
Mus musculus / Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Flotilin1/ TSG101/ Aip1
ELISA
Detected EV-associated proteins
Aip1
Characterization: Particle analysis
NTA
EM
EM-type
cryo EM
Image type
Close-up
|
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EV130137 | 1/3 | Mus musculus Rattus norvegicus/rattus |
Cell culture supernatant | (d)(U)C ExoQuick |
Royo F | 2013 | 0% | |
Study summaryFull title
All authors
Royo F, Schlangen K, Palomo L, Gonzalez E, Conde-Vancells J, Berisa A, Aransay AM, Falcon-Perez JM
Journal
PLoS One
Abstract
The discovery that the cells communicate through emission of vesicles has opened new opportunities f (show more...)
EV-METRIC
0% (median: 23% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C + ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Mus musculus / Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Commercial kit
ExoQuick
Characterization: Particle analysis
|
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EV130137 | 2/3 | Rattus norvegicus/rattus | Serum | (d)(U)C ExoQuick |
Royo F | 2013 | 0% | |
Study summaryFull title
All authors
Royo F, Schlangen K, Palomo L, Gonzalez E, Conde-Vancells J, Berisa A, Aransay AM, Falcon-Perez JM
Journal
PLoS One
Abstract
The discovery that the cells communicate through emission of vesicles has opened new opportunities f (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C + ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Rattus norvegicus/rattus
Sample Type
Serum
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Commercial kit
ExoQuick
Characterization: Particle analysis
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