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You searched for: EV130090 (EV-TRACK ID)

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Experiment number
  • If needed, multiple experiments were identified in a single publication based on differing sample types, separation protocols and/or vesicle types of interest.
Species
  • Species of origin of the EVs.
Separation protocol
  • Gives a short, non-chronological overview of the different steps of the separation protocol.
    • (d)(U)C = (differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
Experiment number
  • Experiments differ in Sample type
Experiment number
  • Experiments differ in Sample type
Details EV-TRACK ID Experiment nr. Species Sample type separation protocol First author Year EV-METRIC
EV130090 1/2 Rattus norvegicus/rattus Cell culture supernatant DG
(d)(U)C
Grapp M 2013 33%

Study summary

Full title
All authors
Grapp M, Wrede A, Schweizer M, Hüwel S, Galla HJ, Snaidero N, Simons M, Bückers J, Low PS, Urlaub H, Gärtner J, Steinfeld R
Journal
Nat Commun
Abstract
Loss of folate receptor-? function is associated with cerebral folate transport deficiency and child (show more...)Loss of folate receptor-? function is associated with cerebral folate transport deficiency and childhood-onset neurodegeneration. To clarify the mechanism of cerebral folate transport at the blood-cerebrospinal fluid barrier, we investigate the transport of 5-methyltetrahydrofolate in polarized cells. Here we identify folate receptor-?-positive intralumenal vesicles within multivesicular bodies and demonstrate the directional cotransport of human folate receptor-?, and labelled folate from the basolateral to the apical membrane in rat choroid plexus cells. Both the apical medium of folate receptor-?-transfected rat choroid plexus cells and human cerebrospinal fluid contain folate receptor-?-positive exosomes. Loss of folate receptor-?-expressing cerebrospinal fluid exosomes correlates with severely reduced 5-methyltetrahydrofolate concentration, corroborating the importance of the folate receptor-?-mediated folate transport in the cerebrospinal fluid. Intraventricular injections of folate receptor-?-positive and -negative exosomes into mouse brains demonstrate folate receptor-?-dependent delivery of exosomes into the brain parenchyma. Our results unravel a new pathway of folate receptor-?-dependent exosome-mediated folate delivery into the brain parenchyma and opens new avenues for cerebral drug targeting. (hide)
EV-METRIC
33% (61st percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • (d)(U)C = (differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
DG + (d)(U)C
Protein markers
EV: Alix/ Flotillin2
non-EV:
Proteomics
yes
EV density (g/ml)
1.13-1.16
Show all info
Study aim
Function
Sample
Species
Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g
Obtain an EV pellet :
Yes
Pelleting: time(min)
150
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ Flotillin2
ELISA
Detected EV-associated proteins
Flotillin2
EV130090 2/2 Homo sapiens "Cerebrospinal fluid" DG
(d)(U)C
Grapp M 2013 33%

Study summary

Full title
All authors
Grapp M, Wrede A, Schweizer M, Hüwel S, Galla HJ, Snaidero N, Simons M, Bückers J, Low PS, Urlaub H, Gärtner J, Steinfeld R
Journal
Nat Commun
Abstract
Loss of folate receptor-? function is associated with cerebral folate transport deficiency and child (show more...)Loss of folate receptor-? function is associated with cerebral folate transport deficiency and childhood-onset neurodegeneration. To clarify the mechanism of cerebral folate transport at the blood-cerebrospinal fluid barrier, we investigate the transport of 5-methyltetrahydrofolate in polarized cells. Here we identify folate receptor-?-positive intralumenal vesicles within multivesicular bodies and demonstrate the directional cotransport of human folate receptor-?, and labelled folate from the basolateral to the apical membrane in rat choroid plexus cells. Both the apical medium of folate receptor-?-transfected rat choroid plexus cells and human cerebrospinal fluid contain folate receptor-?-positive exosomes. Loss of folate receptor-?-expressing cerebrospinal fluid exosomes correlates with severely reduced 5-methyltetrahydrofolate concentration, corroborating the importance of the folate receptor-?-mediated folate transport in the cerebrospinal fluid. Intraventricular injections of folate receptor-?-positive and -negative exosomes into mouse brains demonstrate folate receptor-?-dependent delivery of exosomes into the brain parenchyma. Our results unravel a new pathway of folate receptor-?-dependent exosome-mediated folate delivery into the brain parenchyma and opens new avenues for cerebral drug targeting. (hide)
EV-METRIC
33% (87th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
"Cerebrospinal fluid"
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • (d)(U)C = (differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
DG + (d)(U)C
Protein markers
EV: Flotillin2
non-EV:
Proteomics
yes
EV density (g/ml)
1.11-1.16
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
"Cerebrospinal fluid"
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g
Obtain an EV pellet :
Yes
Pelleting: time(min)
150
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotillin2
ELISA
Detected EV-associated proteins
Flotillin2
Characterization: Particle analysis
EM
EM-type
immune EM
Proteïns
Flotillin2
Image type
Close-up
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