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You searched for: EV130056 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130056 | 1/2 | Bos bovis | Follicular fluid |
(d)(U)C Filtration |
Sohel MM | 2013 | 33% | |
Study summaryFull title
All authors
Sohel MM, Hoelker M, Noferesti SS, Salilew-Wondim D, Tholen E, Looft C, Rings F, Uddin MJ, Spencer TE, Schellander K, Tesfaye D
Journal
PLoS One
Abstract
Cell-cell communication within the follicle involves many signaling molecules, and this process may (show more...)
EV-METRIC
33% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Follicular fluid
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
138.6 (pelleting) / 138.6 (washing)
Protein markers
EV: CD63
non-EV: Cell organelle protein/ Ago2 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Bos bovis
Sample Type
Follicular fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW55
Pelleting: adjusted k-factor
138.6
Wash: Rotor Type
SW55
Wash: adjusted k-factor
138.6
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63
Detected contaminants
Cell organelle protein/ Ago2
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
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EV130056 | 2/2 | Bos bovis | Follicular fluid |
(d)(U)C ExoQuick Filtration |
Sohel MM | 2013 | 25% | |
Study summaryFull title
All authors
Sohel MM, Hoelker M, Noferesti SS, Salilew-Wondim D, Tholen E, Looft C, Rings F, Uddin MJ, Spencer TE, Schellander K, Tesfaye D
Journal
PLoS One
Abstract
Cell-cell communication within the follicle involves many signaling molecules, and this process may (show more...)
EV-METRIC
25% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Follicular fluid
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Filtration Protein markers
EV: CD63
non-EV: Cell organelle protein/ Ago2 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Bos bovis
Sample Type
Follicular fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63
Detected contaminants
Cell organelle protein/ Ago2
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
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1 - 2 of 2 |
EV-TRACK ID | EV130056 | |
---|---|---|
species | Bos bovis | |
sample type | Follicular fluid | |
condition | NAY | |
separation protocol | (d)(U)C Filtration | (d)(U)C ExoQuick Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 33 | 25 |