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You searched for: EV130054 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130054 | 1/1 | Homo sapiens | Cell culture supernatant | DG | Regev-Rudzki N | 2013 | 33% | |
Study summaryFull title
All authors
Regev-Rudzki N, Wilson DW, Carvalho TG, Sisquella X, Coleman BM, Rug M, Bursac D, Angrisano F, Gee M, Hill AF, Baum J, Cowman AF
Journal
Cell
Abstract
Cell-cell communication is an important mechanism for information exchange promoting cell survival f (show more...)
EV-METRIC
33% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
Exosome-like vesicles
Separation protocol
Separation protocol
DG
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
Density gradient
Only used for validation of main results
1
Density medium
Iodixanol
Lowest density fraction
10
Highest density fraction
30
Orientation
Top-down
Characterization: Particle analysis
EM
EM-type
transmission EM/ cryo EM/ atomic force EM
Image type
Close-up, Wide-field
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