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You searched for: EV130028 (EV-TRACK ID)

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Experiment number
  • If needed, multiple experiments were identified in a single publication based on differing sample types, separation protocols and/or vesicle types of interest.
Species
  • Species of origin of the EVs.
Separation protocol
  • Gives a short, non-chronological overview of the different steps of the separation protocol.
    • dUC = differential ultracentrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
Experiment number
  • Experiments differ in Sample type
Experiment number
  • Experiments differ in Sample type
Details EV-TRACK ID Experiment nr. Species Sample type separation protocol First author Year EV-METRIC
EV130028 1/2 Mus musculus Cell culture supernatant DG
dUC
An K 2013 44%

Study summary

Full title
All authors
An K, Klyubin I, Kim Y, Jung JH, Mably AJ, O'Dowd ST, Lynch T, Kanmert D, Lemere CA, Finan GM, Park JW, Kim TW, Walsh DM, Rowan MJ, Kim JH
Journal
Mol Brain
Abstract
BACKGROUND: Exosomes, small extracellular vesicles of endosomal origin, have been suggested to be in (show more...)BACKGROUND: Exosomes, small extracellular vesicles of endosomal origin, have been suggested to be involved in both the metabolism and aggregation of Alzheimer's disease (AD)-associated amyloid ?-protein (A?). Despite their ubiquitous presence and the inclusion of components which can potentially interact with A?, the role of exosomes in regulating synaptic dysfunction induced by A? has not been explored. RESULTS: We here provide in vivo evidence that exosomes derived from N2a cells or human cerebrospinal fluid can abrogate the synaptic-plasticity-disrupting activity of both synthetic and AD brain-derived A?. Mechanistically, this effect involves sequestration of synaptotoxic A? assemblies by exosomal surface proteins such as PrPC rather than A? proteolysis. CONCLUSIONS: These data suggest that exosomes can counteract the inhibitory action of A?, which contributes to perpetual capability for synaptic plasticity. (hide)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = differential ultracentrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
DG + dUC
Protein markers
EV: Alix/ CD81/ Beta-actin/ Flotilin1/ PrP
non-EV:
Proteomics
no
EV density (g/ml)
1.15-1.19
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g
Density gradient
Density medium
Iodixanol
Lowest density fraction
5
Highest density fraction
30
Orientation
Top-down
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD81/ Flotilin1/ PrP/ Beta-actin
ELISA
Detected EV-associated proteins
PrP/ Beta-actin
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Close-up
EV130028 2/2 Homo sapiens "Cerebrospinal fluid" DG
dUC
An K 2013 22%

Study summary

Full title
All authors
An K, Klyubin I, Kim Y, Jung JH, Mably AJ, O'Dowd ST, Lynch T, Kanmert D, Lemere CA, Finan GM, Park JW, Kim TW, Walsh DM, Rowan MJ, Kim JH
Journal
Mol Brain
Abstract
BACKGROUND: Exosomes, small extracellular vesicles of endosomal origin, have been suggested to be in (show more...)BACKGROUND: Exosomes, small extracellular vesicles of endosomal origin, have been suggested to be involved in both the metabolism and aggregation of Alzheimer's disease (AD)-associated amyloid ?-protein (A?). Despite their ubiquitous presence and the inclusion of components which can potentially interact with A?, the role of exosomes in regulating synaptic dysfunction induced by A? has not been explored. RESULTS: We here provide in vivo evidence that exosomes derived from N2a cells or human cerebrospinal fluid can abrogate the synaptic-plasticity-disrupting activity of both synthetic and AD brain-derived A?. Mechanistically, this effect involves sequestration of synaptotoxic A? assemblies by exosomal surface proteins such as PrPC rather than A? proteolysis. CONCLUSIONS: These data suggest that exosomes can counteract the inhibitory action of A?, which contributes to perpetual capability for synaptic plasticity. (hide)
EV-METRIC
22% (56th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
"Cerebrospinal fluid"
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = differential ultracentrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
DG + dUC
Protein markers
EV: Flotilin1/ PrP
non-EV:
Proteomics
no
EV density (g/ml)
1.150
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
"Cerebrospinal fluid"
Separation Method
Differential ultracentrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g
Density gradient
Density medium
Iodixanol
Lowest density fraction
5
Highest density fraction
30
Orientation
Top-down
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotilin1/ PrP
ELISA
Detected EV-associated proteins
PrP
Characterization: Particle analysis
1 - 2 of 2
  • CM = Commercial method
  • dUC = differential ultracentrifugation
  • DG = density gradient
  • UF = ultrafiltration
  • SEC = size-exclusion chromatography
EV-TRACK ID
EV130028
species
Mus musculus
Homo sapiens
sample type
Cell culture
Cerebrospinal fluid
medium
EV Depleted
separation protocol
DG
dUC
DG
dUC
Exp. nr.
1
2
EV-METRIC %
44
22