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You searched for: EV130026 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130026 | 2/3 | Homo sapiens | Urine |
(d)(U)C UF |
Gerlach JQ | 2013 | 38% | |
Study summaryFull title
All authors
Gerlach JQ, Krüger A, Gallogly S, Hanley SA, Hogan MC, Ward CJ, Joshi L, Griffin MD
Journal
PLoS One
Abstract
Urinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomo (show more...)
EV-METRIC
38% (73rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: AQP2/ CD24
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD24/ AQP2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD24/ AQP2
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
|
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EV130026 | 1/3 | Homo sapiens | Urine |
(d)(U)C UF |
Gerlach JQ | 2013 | 33% | |
Study summaryFull title
All authors
Gerlach JQ, Krüger A, Gallogly S, Hanley SA, Hogan MC, Ward CJ, Joshi L, Griffin MD
Journal
PLoS One
Abstract
Urinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomo (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: AQP2/ CD24
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD24/ AQP2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD24/ AQP2
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV130026 | 3/3 | Homo sapiens | Urine |
(d)(U)C DG |
Gerlach JQ | 2013 | 29% | |
Study summaryFull title
All authors
Gerlach JQ, Krüger A, Gallogly S, Hanley SA, Hogan MC, Ward CJ, Joshi L, Griffin MD
Journal
PLoS One
Abstract
Urinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomo (show more...)
EV-METRIC
29% (60th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Adj. k-factor
242.1 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
Surespin630
Pelleting: adjusted k-factor
242.1
Density gradient
Lowest density fraction
5
Highest density fraction
30
Rotor type
Surespin630
Speed (g)
120000
Characterization: Particle analysis
None
|
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1 - 3 of 3 |
EV-TRACK ID | EV130026 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Urine | ||
condition | NAY | ||
separation protocol | (d)(U)C UF | (d)(U)C UF | (d)(U)C DG |
Exp. nr. | 2 | 1 | 3 |
EV-METRIC % | 38 | 33 | 29 |