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You searched for: EV130025 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130025 | 2/2 | Salmonella enterica | Bacteria | DG (d)(U)C Filtration |
Guidi R | 2013 | 78% | |
Study summaryFull title
All authors
Guidi R, Levi L, Rouf SF, Puiac S, Rhen M, Frisan T
Journal
Cell Microbiol
Abstract
Cytolethal-distending toxins (CDTs) belong to a family of DNA damage inducing exotoxins that are pro (show more...)
EV-METRIC
78% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Cell Name
DNF
Sample origin
DNF
Focus vesicles
OMV
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Adj. k-factor
602 (pelleting) / 602 (washing)
Protein markers
EV:
non-EV: Cell organelle protein Proteomics
no
EV density (g/ml)
1.130
TEM measurements
50
Show all info
Study aim
Function
Sample
Species
Salmonella enterica
Sample Type
Bacteria
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Pelleting: rotor type
TH641
Pelleting: adjusted k-factor
602.0
Wash: Rotor Type
TH641
Wash: adjusted k-factor
602.0
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.2
Highest density fraction
2
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
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EV130025 | 1/2 | Salmonella enterica | Cell culture supernatant | (d)(U)C Filtration |
Guidi R | 2013 | 22% | |
Study summaryFull title
All authors
Guidi R, Levi L, Rouf SF, Puiac S, Rhen M, Frisan T
Journal
Cell Microbiol
Abstract
Cytolethal-distending toxins (CDTs) belong to a family of DNA damage inducing exotoxins that are pro (show more...)
EV-METRIC
22% (45th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C + Filtration
Adj. k-factor
602 (pelleting) / 602 (washing)
Protein markers
EV: MHC1
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Function
Sample
Species
Salmonella enterica
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Pelleting: rotor type
TH641
Pelleting: adjusted k-factor
602.0
Wash: volume per pellet (ml)
10
Wash: Rotor Type
TH641
Wash: adjusted k-factor
602.0
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
MHC1
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
MHC1
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
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