Search > Results
You searched for: EV130015 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130015 | 1/1 | Homo sapiens | Urine | (d)(U)C | Musante L | 2013 | 44% | |
Study summaryFull title
All authors
Musante L, Saraswat M, Ravidà A, Byrne B, Holthofer H
Journal
Nephrol Dial Transplant
Abstract
BACKGROUND: Urinary vesicles represent a newly established source of biological material, widely con (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
Nano(-sized) vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
78.45 (pelleting)
Protein markers
EV: CD81/ TSG101/ Beta-actin/ AQP2/ Podocin
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
78.45
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ TSG101/ AQP2/ Podocin/ Beta-actin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
AQP2/ Podocin/ Beta-actin
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV130015 |
---|---|
species | Homo sapiens |
sample type | Urine |
condition | NAY |
separation protocol | (d)(U)C |
Exp. nr. | 1 |
EV-METRIC % | 44 |