Search > Results
You searched for: EV120041 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120041 | 1/1 | Bos bovis | Milk | DG (d)(U)C Filtration |
Reinhardt TA | 2012 | 56% | |
Study summaryFull title
All authors
Reinhardt TA, Lippolis JD, Nonnecke BJ, Sacco RE
Journal
J Proteomics
Abstract
Exosomes are 40-100 nm membrane vesicles of endocytic origin, secreted by cells and are found in bio (show more...)
EV-METRIC
56% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Adj. k-factor
157.1 (pelleting)
Protein markers
EV: TSG101/ MFGE8
non-EV: Proteomics
yes
EV density (g/ml)
1.7
Show all info
Study aim
Omics
Sample
Species
Bos bovis
Sample Type
Milk
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Pelleting: rotor type
50.2Ti
Pelleting: adjusted k-factor
157.1
Density gradient
Density medium
Sucrose
Lowest density fraction
5
Highest density fraction
43
Orientation
Bottom-up
Rotor type
50.2Ti
Speed (g)
200000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
TSG101/ MFGE8
ELISA
Detected EV-associated proteins
MFGE8
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
1 - 1 of 1 |