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You searched for: EV120013 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120013 | 1/1 | Homo sapiens | Cell culture supernatant | DG (d)(U)C |
Choi DS | 2012 | 50% | |
Study summaryFull title
All authors
Choi DS, Choi DY, Hong BS, Jang SC, Kim DK, Lee J, Kim YK, Kim KP, Gho YS
Journal
J Extracell Vesicles
Abstract
Cancer cells actively release extracellular vesicles (EVs), including exosomes and microvesicles, in (show more...)
EV-METRIC
50% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: CD63/ LAMP1/ CD81/ Alix/ ICAM1/ Beta-actin/ CD9
non-EV: Proteomics
yes
EV density (g/ml)
1.08-1.1
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Density gradient
Density medium
Iodixanol
Lowest density fraction
5
Highest density fraction
30
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ CD81/ CD9/ LAMP1/ ICAM1/ Beta-actin
ELISA
Detected EV-associated proteins
LAMP1/ ICAM1/ Beta-actin
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
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