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You searched for: EV120007 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120007 | 1/2 | Homo sapiens | Cell culture supernatant | DG (d)(U)C |
Lugini L | 2012 | 78% | |
Study summaryFull title
All authors
Lugini L, Cecchetti S, Huber V, Luciani F, Macchia G, Spadaro F, Paris L, Abalsamo L, Colone M, Molinari A, Podo F, Rivoltini L, Ramoni C, Fais S
Journal
J Immunol
Abstract
Exosomes are nanovesicles released by normal and tumor cells, which are detectable in cell culture s (show more...)
EV-METRIC
78% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Adj. k-factor
41.45 (pelleting)
Protein markers
EV: NKG2D/ CD63/ MHC1/ CD56/ Rab5b
non-EV: Cell organelle protein Proteomics
no
EV density (g/ml)
1.1-1.19
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Pelleting: rotor type
TLA100.2
Pelleting: adjusted k-factor
41.45
Density gradient
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Bottom-up
Rotor type
SW41
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ Rab5b/ MHC1/ CD56/ NKG2D
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
Rab5b/ MHC1/ CD56/ NKG2D
Characterization: Particle analysis
EM
EM-type
immune EM
Proteïns
Rab5b; CD56
Image type
Close-up, Wide-field
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EV120007 | 2/2 | Homo sapiens | Blood plasma | DG (d)(U)C Filtration |
Lugini L | 2012 | 67% | |
Study summaryFull title
All authors
Lugini L, Cecchetti S, Huber V, Luciani F, Macchia G, Spadaro F, Paris L, Abalsamo L, Colone M, Molinari A, Podo F, Rivoltini L, Ramoni C, Fais S
Journal
J Immunol
Abstract
Exosomes are nanovesicles released by normal and tumor cells, which are detectable in cell culture s (show more...)
EV-METRIC
67% (95th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Adj. k-factor
37.69 (pelleting)
Protein markers
EV: NKG2D/ Perforin/ CD56/ Rab5b
non-EV: CD3/ CD8/ NKp46/ NKp44/ CD4 Proteomics
no
EV density (g/ml)
1.19-1.23
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
TLA100.2
Pelleting: adjusted k-factor
37.69
Density gradient
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Bottom-up
Rotor type
SW41
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Rab5b/ Perforin/ CD56/ NKG2D
Detected contaminants
"NKp46/ NKp44/ CD3/ CD4/ CD8"
ELISA
Detected EV-associated proteins
Rab5b/ Perforin/ CD56/ NKG2D
Characterization: Particle analysis
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