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You searched for: EV120004 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120004 | 1/1 | Homo sapiens | Cell culture supernatant | DG (d)(U)C |
de Jong OG | 2012 | 67% | |
Study summaryFull title
All authors
de Jong OG, Verhaar MC, Chen Y, Vader P, Gremmels H, Posthuma G, Schiffelers RM, Gucek M, van Balkom BW
Journal
J Extracell Vesicles
Abstract
BACKGROUND: The healthy vascular endothelium, which forms the barrier between blood and the surround (show more...)
EV-METRIC
67% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Adj. k-factor
256 (pelleting)
Protein markers
EV: HSP70/ Beta-actin/ Flotilin1/ CD63/ ICAM1
non-EV: Proteomics
yes
EV density (g/ml)
1.09-1.12
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW32;SW60
Pelleting: adjusted k-factor
256.0
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ Flotilin1/ HSP70/ Beta-actin/ ICAM1
ELISA
Detected EV-associated proteins
Beta-actin/ ICAM1
Characterization: Particle analysis
NTA
EM
EM-type
immune EM
Proteïns
CD63
Image type
Wide-field
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