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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV110053	1/1	Homo sapiens	Saliva	(d)(U)C Filtration Gel filtration UF	Ogawa Y	2011	11%
Study summary Full title Proteomic analysis of two types of exosomes in human whole saliva All authors Ogawa Y, Miura Y, Harazono A, Kanai-Azuma M, Akimoto Y, Kawakami H, Yamaguchi T, Toda T, Endo T, Tsubuki M, Yanoshita R Journal Biol Pharm Bull Abstract Saliva contains a large number of proteins that participate in the protection of oral tissue. Exosom (show more...)Saliva contains a large number of proteins that participate in the protection of oral tissue. Exosomes are small vesicles (30-100 nm in diameter) with an endosome-derived limiting membrane that are secreted by a diverse range of cell types. We have recently demonstrated that exosomes are present in human whole saliva. In this study, we found that whole saliva contained at least two types of exosomes (exosome I and exosome II) that are different in size and protein composition. Proteomic analysis revealed that both types of exosomes contained Alix, Tsg101 and Hsp70, all exosomal markers, immunoglobulin A and polymeric immunoglobulin receptor, whereas they had different protein compositions. Most of dipeptidyl peptidase IV known as CD26 in whole saliva, was present on the exosome II and metabolically active in cleaving chemokines (CXCL11 and CXCL12). Human whole saliva exosomes might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity. (hide) EV-METRIC 11% (29th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Saliva Sample origin NAY Focus vesicles exosomes Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (d)(U)C Filtration Gel filtration UF Protein markers EV: TSG101/ CD63/ Galectin3/ Alix/ Actin/ DPP IV/ HSP70 non-EV: Proteomics yes Show all info Study aim Omics Sample Species Homo sapiens Sample Type Saliva Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 10,000 g and 50,000 g Pelleting performed No Other Name other separation method Gel filtration Characterization: Protein analysis Western Blot Detected EV-associated proteins Alix/ CD63/ HSP70/ TSG101/ "Actin/ Galectin3/ DPP IV" ELISA Detected EV-associated proteins "Actin/ Galectin3/ DPP IV" Characterization: Particle analysis EM EM-type immune EM Image type Wide-field

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EV-TRACK ID	EV110053
species	Homo sapiens
sample type	Saliva
condition	NAY
separation protocol	(d)(U)C Filtration Gel filtration UF
Exp. nr.	1
EV-METRIC %	11