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You searched for: EV110050 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110050 | 1/2 | Rattus norvegicus/rattus | Cell culture supernatant | DG (d)(U)C IAF |
Müller G | 2011 | 43% | |
Study summaryFull title
All authors
Müller G, Schneider M, Biemer-Daub G, Wied S
Journal
Cell Signal
Abstract
Small microvesicles, such as microparticles and exosomes, have been demonstrated to transfer protein (show more...)
EV-METRIC
43% (70th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
microvesicles
Separation protocol
Separation protocol
DG + (d)(U)C + IAF
Adj. k-factor
9.06 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.22
Show all info
Study aim
Function
Sample
Species
Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
30
Pelleting: rotor type
A110
Pelleting: adjusted k-factor
9.06
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
10
Highest density fraction
70
Orientation
Top-down
Speed (g)
100000
|
||||||||
EV110050 | 2/2 | Rattus norvegicus/rattus | Serum | DG (d)(U)C IAF |
Müller G | 2011 | 29% | |
Study summaryFull title
All authors
Müller G, Schneider M, Biemer-Daub G, Wied S
Journal
Cell Signal
Abstract
Small microvesicles, such as microparticles and exosomes, have been demonstrated to transfer protein (show more...)
EV-METRIC
29% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Cell Name
DNF
Sample origin
DNF
Focus vesicles
microvesicles
Separation protocol
Separation protocol
DG + (d)(U)C + IAF
Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.22
Show all info
Study aim
Function
Sample
Species
Rattus norvegicus/rattus
Sample Type
Serum
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
10
Highest density fraction
70
Orientation
Top-down
Speed (g)
100000
|
||||||||
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