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You searched for: EV110005 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110005 | 1/1 | Rattus norvegicus/rattus | Cell culture supernatant | DG (d)(U)C |
Carayon K | 2011 | 44% | |
Study summaryFull title
All authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé L, Joly E
Journal
J Biol Chem
Abstract
During the orchestrated process leading to mature erythrocytes, reticulocytes must synthesize large (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: TSG101/ CHMP4C/ HSC70/ CHMP4B/ HSP90/ MHC1
non-EV: Proteomics
yes
EV density (g/ml)
1.16-1.21
Show all info
Study aim
Omics
Sample
Species
Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.5
Highest density fraction
2.5
Orientation
Top-down
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
HSP90/ TSG101/ HSC70/ CHMP4B/ CHMP4C/ MHC1
ELISA
Detected EV-associated proteins
HSC70/ CHMP4B/ CHMP4C/ MHC1
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
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