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You searched for: EV110001 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110001 | 1/1 | Homo sapiens | Cell culture supernatant | DG (d)(U)C |
Atay S | 2011 | 78% | |
Study summaryFull title
All authors
Atay S, Gercel-Taylor C, Kesimer M, Taylor DD
Journal
Exp Cell Res
Abstract
Exosomes represent an important intercellular communication vehicle, mediating events essential for (show more...)
EV-METRIC
78% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: CD81/ Alix/ ICAM1/ HSP70/ Beta-actin/ HSP60
non-EV: Cell organelle protein Proteomics
yes
EV density (g/ml)
1.13-1.17
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Density gradient
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Rotor type
90Ti
Speed (g)
105000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD81/ HSP70/ Beta-actin/ ICAM1/ HSP60
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
Beta-actin/ ICAM1/ HSP60
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
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