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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV100086	1/1	Homo sapiens	Homogenized skeletal metastases	(d)(U)C SEC	Ronquist KG	2010	14%
Study summary Full title Proteomic analysis of prostate cancer metastasis-derived prostasomes All authors Ronquist KG, Ronquist G, Larsson A, Carlsson L Journal Anticancer Res Abstract BACKGROUND: The secretory epithelial cells of the prostate gland use sophisticated vehicles in the f (show more...)BACKGROUND: The secretory epithelial cells of the prostate gland use sophisticated vehicles in the form of prostasomes to relay important information to sperm cells in semen. This prostasome-forming and secretory ability of the epithelial cells is also preserved in poorly differentiated prostate cancer cells. The aim of the present investigation was to conduct a proteomic analysis of metastasis-derived prostasomes. MATERIALS AND METHODS: We investigated prostasomes from vertebral metastases of prostate cancer by 2-dimensional electrophoresis and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) protein characterization. RESULTS: Twenty-five unique protein spots were identified by MALDI-TOF and another five proteins were determined by mass spectrometry (MS)/MS. Annexins A1, A3 and A5, as well as dimethylarginine dimethylaminohydrolase 1 were among the identified proteins. The annexins and dimethylarginine dimethylaminohydrolase 1 found in cancer-derived prostasomes can act, among others, as angiogenic factors and can increase the vascular development in the neighbourhood of the tumour. CONCLUSION: Cancer-derived prostasomes may play an important role in the interaction between tumour cells and their environment. (hide) EV-METRIC 14% (50th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Homogenized skeletal metastases Sample origin NAY Focus vesicles Prostasomes Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (d)(U)C SEC Protein markers EV: non-EV: Proteomics yes Show all info Study aim Omics Sample Species Homo sapiens Sample Type Homogenized skeletal metastases Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed Yes Pelleting: time(min) 120 Characterization: Protein analysis

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EV-TRACK ID	EV100086
species	Homo sapiens
sample type	Homogenized skeletal metastases
condition	NAY
separation protocol	(d)(U)C SEC
Exp. nr.	1
EV-METRIC %	14